cDNA Clone NFkB p65 (Rel A)

• Plasmid: pFastBac.HTb p65
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-p65
• Genbank: M62399
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGACGAACTGTTCCCCCTCATCTTCCCGGCAGAGCCAGCCCAG
GCCTCTGGCCCCTATGTGGAGATCATTGAGCAGCCCAAGCAGCGGGGCAT
GCGCTTCCGCTACAAGTGCGAGGGGCGCTCCGCGGGCAGCATCCCAGGCG
AGAGGAGCACAGATACCACCAAGACCCACCCCACCATCAAGATCAATGGC
TACACAGGACCAGGGACAGTGCGCATCTCCCTGGTCACCAAGGACCCTCC
TCACCGGCCTCACCCCCACGAGCTTGTAGGAAAGGACTGCCGGGATGGCT
TCTATGAGGCTGAGCTCTGCCCGGACCGCTGCATCCACAGTTTCCAGAAC
CTGGGAATCCAGTGTGTGAAGAAGCGGGACCTGGAGCAGGCTATCAGTCA
GCGCATCCAGACCAACAACAACCCCTTCCAAGTTCCTATAGAAGAGCAGC
GTGGGGACTACGACCTGAATGCTGTGCGGCTCTGCTTCCAGGTGACAGTG
CGGGACCCATCAGGCAGGCCCCTCCGCCTGCCGCCTGTCCTTTCTCATCC
CATCTTTGACAATCGTGCCCCCAACACTGCCGAGCTCAAGATCTGCCGAG
TGAACCGAAACTCTGGCAGCTGCCTCGGTGGGGATGAGATCTTCCTACTG
TGTGACAAGGTGCAGAAAGAGGACATTGAGGTGTATTTCACGGGACCAGG
CTGGGAGGCCCGAGGCTCCTTTTCGCAAGCTGATGTGCACCGACAAGTGG
CCATTGTGTTCCGGACCCCTCCCTACGCAGACCCCAGCCTGCAGGCTCCT
GTGCGTGTCTCCATGCAGCTGCGGCGGCCTTCCGACCGGGAGCTCAGTGA
GCCCATGGAATTCCAGTACCTGCCAGATACAGACGATCGTCACCGGATTG
AGGAGAAACGTAAAAGGACATATGAGACCTTCAAGAGCATCATGAAGAAG
AGTCCTTTCAGCGGACCCACCGACCCCCGGCCTCCACCTCGACGCATTGC
TGTGCCTTCCCGCAGCTCAGCTTCTGTCCCCAAGCCAGCACCCCAGCCCT
ATCCCTTTACGTCATCCCTGAGCACCATCAACTATGATGAGTTTCCCACC
ATGGTGTTTCCTTCTGGGCAGATCAGCCAGGCCTCGGCCTTGGCCCCGGC
CCCTCCCCAAGTCCTGCCCCAGGCTCCAGCCCCTGCCCCTGCTCCAGCCA
TGGTATCAGCTCTGGCCCAGGCCCCAGCCCCTGTCCCAGTCCTAGCCCCA
GGCCCTCCTCAGGCTGTGGCCCCACCTGCCCCCAAGCCCACCCAGGCTGG
GGAAGGAACGCTGTCAGAGGCCCTGCTGCAGCTGCAGTTTGATGATGAAG
ACCTGGGGGCCTTGCTTGGCAACAGCACAGATCCAGCTGTGTTCACAGAC
CTGGCATCCGTCGACAACTCCGAGTTTCAGCAGCTGCTGAACCAGGGCAT
ACCTGTGGCCCCCCACACAACAGAGCCCATGCTGATGGAGTACCCTGAGG
CTATAACTCGCCTAGTGACAGGGGCCCAGAGGCCCCCCGACCCAGCTCCT
GCTCCACTGGGGGCCCCGGGGCTCCCCAATGGCCTCCTTTCAGGAGATGA
AGACTTCTCCTCCATTGCGGACATGGACTTCTCAGCCCTGCTGAGTCAGA
TCAGCTCCTAAGGTACC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1005
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag