cDNA Clone TTBK1

• Synonyms: BDTK
• Plasmid: pCMVD FLAG TTBK1 full Kdead D176/A
• Parent Plasmid: pCMV5D FLAG
• Expressed: FLAG-TTBK1 full Kdead D176/A
• Genbank: NM_032538.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGCAGTGCCTAGCGGCAGCTCTTAAGGACGAAACCAACATGAGTGGGGGAGGGGAGCAGGCCGACATCCTGCCGGCCAACTACGTGGTCAAAGATCGCTGGAAGGTGCTGAAAAAGATCGGGGGCGGGGGCTTCGGTGAGATCTATGAGGCCATGGACCTGCTGACCAGGGAGAACGTGGCTCTCAAGGTGGAGTCAGCCCAACAGCCTAAGCAGGTCCTCAAGATGGAGGTGGCTGTGCTCAAAAAGCTTCAAGGCAAGGACCACGTGTGCAGGTTCATTGGCTGTGGCCGTAACGAGAAGTTTAACTATGTGGTGATGCAGCTCCAGGGCCGGAACCTGGCTGACCTGCGCCGCAGCCAGCCAAGGGGCACTTTCACGTTGAGTACCACACTGCGCCTGGGCAAGCAGATCCTGGAGTCCATTGAAGCCATTCACTCCGTGGGCTTCCTGCACCGTGACATCAAGCCGTCCAACTTTGCCATGGGCCGGCTGCCCTCGACCTACAGGAAGTGCTACATGTTGGCCTTTGGGCTAGCCAGGCAGTACACTAACACCACTGGGGACGTCCGGCCTCCTCGGAATGTGGCCGGGTTCCGGGGGACTGTCCGCTACGCCTCGGTCAATGCTCACAAGAACCGGGAGATGGGCCGCCATGATGACCTGTGGTCCCTCTTCTACATGCTGGTGGAGTTTGCTGTGGGCCAGCTGCCCTGGAGGAAGATCAAAGACAAGGAGCAGGTGGGGATGATCAAAGAGAAATACGAGCACCGGATGCTACTGAAGCACATGCCATCCGAGTTTCATCTCTTCCTGGACCACATTGCCAGCCTCGACTACTTCACCAAGCCCGATTACCAGTTGATCATGTCAGTGTTTGAGAACAGCATGAAGGAGCGGGGCATTGCCGAGAACGAGGCCTTTGACTGGGAGAAGGCAGGCACTGATGCCCTCCTGTCCACGAGCACCTCCACCCCACCCCAGCAGAACACGCGACAGACCGCAGCCATGTTTGGAGTGGTCAATGTGACACCAGTGCCAGGGGATCTGCTGAGGGAAAATACCGAAGACGTGCTCCAGGGGGAACATCTGTCCGACCAGGAAAACGCTCCACCAATCCTGCCTGGAAGACCATCTGAGGGCCTCGGACCTAGTCCACACCTGGTGCCACATCCTGGAGGACCAGAAGCAGAAGTGTGGGAGGAAACCGACGTGAACCGAAATAAGCTGAGGATCAACATTGGCAAAAGCCCTTGCGTGGAGGAAGAGCAGTCCAGGGGAATGGGTGTGCCAAGCTCCCCTGTCAGAGCTCCCCCTGATAGCCCAACCACACCCGTGCGCTCCCTGCGATACAGGAGAGTCAATTCTCCTGAAAGTGAGCGACTCTCTACAGCAGACGGAAGGGTGGAACTGCCAGAGCGGCGCAGCCGAATGGATCTCCCTGGCAGTCCATCACGACAGGCCTGTTCTAGTCAGCCTGCTCAGATGCTGTCCGTGGACACTGGTCACGCCGATAGGCAGGCTTCTGGCAGAATGGACGTGAGCGCCTCCGTCGAACAGGAGGCACTGTCAAACGCCTTCAGAAGCGTGCCACTCGCCGAAGAGGAAGACTTTGATAGCAAGGAGTGGGTGATCATTGACAAGGAAACCGAGCTGAAAGATTTCCCACCAGGAGCAGAGCCATCTACAAGTGGAACTACCGATGAGGAACCTGAGGAACTGCGGCCACTCCCAGAGGAAGGAGAGGAACGAAGGAGACTGGGTGCAGAGCCTACAGTGCGCCCACGAGGCAGGAGCATGCAGGCACTCGCCGAGGAAGACCTGCAGCACCTCCCTCCACAGCCACTCCCACCTCAGCTGTCCCAGGGAGATGGACGCTCAGAGACCAGCCAGCCACCCACACCCGGTTCACCTAGCCACTCCCCACTGCATTCAGGACCTCGGCCACGGCGCCGAGAGTCCGACCCAACCGGACCACAGCGACAGGTGTTCTCCGTCGCTCCTCCATTTGAGGTCAATGGACTGCCAAGGGCAGTCCCTCTCAGCCTGCCATACCAGGATTTCAAGAGAGACCTGTCCGATTATAGAGAGCGAGCACGACTGCTCAACCGGGTGAGGAGAGTCGGATTTTCTCATATGCTGCTCACAACTCCTCAGGTGCCACTGGCACCCGTCCAGCCTCAGGCCAATGGGAAAGAGGAGGAAGAAGAGGAAGAGGAAGACGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGGAAGAAGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGCCGCTGCAGCCGTGGCCCTCGGAGAGGTCCTGGGGCCTAGAAGCGGTTCAAGCTCCGAAGGCAGCGAGAGATCTACTGACAGGAGCCAGGAGGGAGCTCCAAGCACCCTGCTCGCAGACGATCAGAAGGAGTCCCGAGGCAGGGCCTCTATGGCTGACGGAGATCTGGAGCCTGAAGAGGGGTCTAAAACTCTCGTGCTGGTCAGTCCAGGCGACATGAAGAAATCACCCGTGACCGCAGAGCTCGCACCCGACCCTGATCTGGGAACTCTCGCTGCACTGACCCCACAGCACGAGAGGCCACAGCCAACAGGTAGTCAGCTGGACGTGAGCGAACCTGGCACTCTCTCTAGTGTCCTGAAGTCCGAGCCAAAACCACCTGGACCTGGTGCTGGACTGGGAGCAGGGACTGTGACCACAGGAGTCGGGGGTGTGGCTGTCACATCAAGCCCTTTCACTAAGGTGGAACGCACCTTTGTCCACATCGCCGAGAAAACCCATCTGAACGTGATGTCCTCTGGCGGACAGGCTCTCAGGAGCGAAGAGTTTTCCGCAGGAGGTGAACTCGGACTGGAGCTCGCTTCCGACGGAGGAGCAGTGGAAGAGGGAGCTAGAGCACCCCTGGAGAACGGGCTGGCCCTCTCTGGACTCAATGGGGCTGAAATTGAGGGCAGTGCCCTGTCAGGAGCTCCACGAGAAACACCTTCCGAGATGGCTACTAACTCTCTCCCAAATGGACCCGCCCTGGCTGATGGACCTGCCCCAGTGAGCCCACTGGAACCATCCCCTGAGAAGGTCGCAACCATCAGCCCTCGGCGCCATGCTATGCCAGGCTCTAGACCCCGGAGTCGCATTCCTGTGCTGCTCAGTGAAGAGGACACAGGGTCAGAACCATCTGGTAGTCTGTCAGCCAAGGAGCGGTGGTCTAAACGAGCAAGGCCTCAGCAGGATCTCGCCCGCCTGGTGATGGAGAAGAGGCAGGGAAGACTGCTCCTGAGGCTGGCTAGCGGGGCAAGTTCAAGCTCCTCTGAAGAGCAGCGAAGGGCTTCAGAGACACTGAGCGGTACTGGCTCCGAAGAGGACACACCTGCATCCGAGCCAGCCGCTGCACTGCCACGAAAATCTGGCCGAGCCGCTGCAACTAGAAGTAGAATCCCCCGGCCCATTGGACTGCGCATGCCTATGCCAGTGGCCGCTCAGCAGCCTGCTAGCAGATCCCACGGAGCAGCACCAGCACTGGACACTGCCATCACCTCTCGACTGCAGCTCCAGACCCCACCAGGCAGTGCCACAGCTGCAGATCTGAGGCCAAAGCAGCCTCCAGGACGAGGACTCGGTCCAGGAAGGGCACAGGCAGGAGCTAGACCCCCTGCACCTCGGTCCCCACGACTGCCAGCATCTACAAGTGCCGCTAGAAACGCATCAGCCAGCCCACGGTCCCAGTCTCTGAGTAGACGGGAGTCACCCAGCCCTTCCCATCAGGCACGACCAGGAGTGCCACCACCTCGAGGTGTCCCACCCGCTCGAGCACAGCCAGACGGAACCCCATCTCCAGGAGGTAGTAAGAAAGGTCCCAGGGGCAAGCTGCAGGCCCAGAGGGCAACTACTAAAGGTCGGGCAGGAGGTGCTGAAGGAAGGGCAGGGGCAAGGTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMQCLAAALKDETNMSGGGEQADILPANYVVKDRWKVLKKIGGGGFGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDH
VCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLAFGLARQYTNTTGD
VRPPRNVAGFRGTVRYASVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIASLDYFTKPDYQLI
MSVFENSMKERGIAENEAFDWEKAGTDALLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDLLRENTEDVLQGEHLSDQENAPPILPGRPSEGLGPSPHLV
PHPGGPEAEVWEETDVNRNKLRINIGKSPCVEEEQSRGMGVPSSPVRAPPDSPTTPVRSLRYRRVNSPESERLSTADGRVELPERRSRMDLPGSPSRQAC
SSQPAQMLSVDTGHADRQASGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFPPGAEPSTSGTTDEEPEELRPLPEEGEERRRLGA
EPTVRPRGRSMQALAEEDLQHLPPQPLPPQLSQGDGRSETSQPPTPGSPSHSPLHSGPRPRRRESDPTGPQRQVFSVAPPFEVNGLPRAVPLSLPYQDFK
RDLSDYRERARLLNRVRRVGFSHMLLTTPQVPLAPVQPQANGKEEEEEEEEDEEEEEEDEEEEEEEEEEEEEEEEEEEEEEEAAAAVALGEVLGPRSGSS
SEGSERSTDRSQEGAPSTLLADDQKESRGRASMADGDLEPEEGSKTLVLVSPGDMKKSPVTAELAPDPDLGTLAALTPQHERPQPTGSQLDVSEPGTLSS
VLKSEPKPPGPGAGLGAGTVTTGVGGVAVTSSPFTKVERTFVHIAEKTHLNVMSSGGQALRSEEFSAGGELGLELASDGGAVEEGARAPLENGLALSGLN
GAEIEGSALSGAPRETPSEMATNSLPNGPALADGPAPVSPLEPSPEKVATISPRRHAMPGSRPRSRIPVLLSEEDTGSEPSGSLSAKERWSKRARPQQDL
ARLVMEKRQGRLLLRLASGASSSSSEEQRRASETLSGTGSEEDTPASEPAAALPRKSGRAAATRSRIPRPIGLRMPMPVAAQQPASRSHGAAPALDTAIT
SRLQLQTPPGSATAADLRPKQPPGRGLGPGRAQAGARPPAPRSPRLPASTSAARNASASPRSQSLSRRESPSPSHQARPGVPPPRGVPPARAQPDGTPSP
GGSKKGPRGKLQAQRATTKGRAGGAEGRAGAR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU34542
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: D176A Kinase Dead.
• Low Temp: No