cDNA Clone MNK1

• Synonyms: MKNK1
• Plasmid: pEBG6P-MNK1-6His (lacking inactivating insert)
• Parent Plasmid: pEBG6P
• Expressed: GST-MNK1-6His
• Genbank: AB000409 (lacks an inactivating 41 aa insertion in the catalytic domain that is present in some MNK1 mRNA sequences)
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGTATCTTCTCAAAAGTTGGAAAAACCTATAGAGATGGGCAGTAG
CGAACCCCTTCCCATCGCAGATGGTGACAGGAGGAGGAAGAAGAAGCGGA
GGGGCCGGGCCACTGACTCCTTGCCAGGAAAGTTTGAAGATATGTACAAG
CTGACCTCTGAATTGCTTGGAGAGGGAGCCTATGCCAAAGTTCAAGGTGC
CGTGAGCCTACAGAATGGCAAAGAGTATGCCGTCAAAATCATCGAGAAAC
AAGCAGGGCACAGTCGGAGTAGGGTGTTTCGAGAGGTGGAGACGCTGTAT
CAGTGTCAGGGAAACAAGAACATTTTGGAGCTGATTGAGTTCTTTGAAGA
TGACACAAGGTTTTACTTGGTCTTTGAGAAATTGCAAGGAGGTTCCATCT
TAGCCCACATCCAGAAGCAAAAGCACTTCAATGAGCGAGAAGCCAGCCGA
GTGGTGCGGGACGTTGCTGCTGCCCTTGACTTCCTGCATACCAAAGGCAT
TGCTCATCGTGATCTGAAACCAGAAAATATATTGTGTGAATCTCCAGAAA
AGGTGTCTCCAGTGAAAATCTGTGACTTTGACTTGGGCAGTGGGATGAAA
CTGAACAACTCCTGTACCCCCATAACCACACCAGAGCTGACCACCCCATG
TGGCTCTGCAGAATACATGGCCCCTGAGGTAGTGGAGGTCTTCACGGACC
AGGCCACATTCTACGACAAGCGCTGTGACCTGTGGAGCCTGGGCGTGGTC
CTCTACATCATGCTGAGTGGCTACCCACCCTTCGTGGGTCACTGCGGGGC
CGACTGTGGCTGGGACCGGGGCGAGGTCTGCAGGGTGTGCCAGAACAAGC
TGTTTGAAAGCATCCAGGAAGGCAAGTATGAGTTTCCTGACAAGGACTGG
GCACACATCTCCAGTGAAGCCAAAGACCTCATCTCCAAGCTCCTGGTGCG
AGATGCAAAGCAGAGACTTAGCGCCGCCCAAGTTCTGCAGCACCCATGGG
TGCAGGGGCAAGCTCCAGAAAAGGGACTCCCCACGCCGCAAGTCCTCCAG
AGGAACAGCAGCACAATGGACCTGACGCTCTTCGCAGCTGAGGCCATCGC
CCTTAACCGCCAGCTATCTCAGCACGAAGAGAACGAACTAGCAGAGGAGC
CAGAGGCACTAGCTGATGGCCTCTGCTCCATGAAGCTTTCCCCTCCCTGC
AAGTCACGCCTGGCCCGGAGACGGGCCCTGGCCCAGGCAGGCCGTGGTGA
AGACAGGAGCCCGCCCACAGCACTCCATCACCATCACCATCACTGAgaattcccgggtcgactcgagcggccgc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSVSSQKLEKPIEMG
SSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQ
GAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFF
EDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTK
GIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTT
PCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHC
GADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLL
VRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEA
IALNRQLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGR
GEDRSPPTALHHHHHH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16010
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express MNK1 with N-terminal GST (contains preScission protease site) and C-terminal 6His.; ; Estimated MW of expressed protein : 75509 Daltons