cDNA Clone GFP

• Plasmid: pcDNA4/TO AW SCD-AMAXA GFP
• Parent Plasmid: pcDNA4/TO
• Tag: GFP

Sequence of Insert

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATC
TGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTT
GGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAG
GCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCG
CTGCTTCGCGATGTACGGGCCAGATATACGCGAATTCGGATCCGAATTCG
CCCTTGCCCCGTGAGAATATAGAAACAAGGCTGAGAGGCAGCGAAGAGTA
AGGGAGGAGCCAGCACTTACGCATTTTTCACTCTCCTCCTCACTCCCTAG
GGTCATAGTTTTGATTCTGAAGTTAGAAGTGTGGGGGTCGTCGGCTGCAT
TTTCAACTTGGCCTAAAAGTTGGAGTTCACCACACTGCAGGCGCTAGGAA
CTCTGCCGCTCGGTCACCAACCTGAGCCTCTGGCTAGTCCGAAGAACGCG
AAAAGCCAGGCCCAAGGCACTGGTTGTCATGGCAACCGGGGCGCTGGTCT
CCCTGGCGACCTCGTCCCTAGGGAGGAGGTGGAGCTGGATGCCAGGCGGG
CCAATGAGGTCGAGGGGAGCTCGGGGTGGGAGATCTGCGGTCCTCCCAGT
GCCGGGGAATGGTTGAGTGACAGGACCGGGGGGGCGGAGCCGGCGCGGCT
GCCCGAGGTGGAGCCCCAGTGGTGCGAGTAGCTCCAGCGGCCACGCTGAG
GCGAGGGTGACACACCATGCTCACGGCCCCTGGAGACCGTCGTGCTGGCG
AGCCGTGCTCCGTAGCTCCCCGGTCCGCCTCGGCAGCGGTCAGAGTCGCC
TACAGGAGTTGAGCCGCCCGCGCCAGAAGGTTTTGGCGAAGCTCTTGGAG
AGGCGTCGAGCACAGTAGGGCGGCGGGGGTGCGTTGAGCGCTCGGGGGTC
AGGCAGTCGGCCGGGATCGCCGCTGGGGAGCGTTTCCGAGGCGAGGAGGA
GGAGGAGGAGATGCTGCTCCTCTTCTCCCCCTCCCCAGGCTCCTTCTACA
GCTCCTTCAGCCCACGCCCGCAGCCGCTTGTGGGAGAAGTGGTGGTGGCT
CTCGGCGCCCGCGGCGGCTGCCGGCGGCCCGCCCCGACGCCGCGTCCCTG
CAGCCCTCGCCCGGCGCTCCAGTAGCAGGACCCGGTCTCGGGACCAGCCG
GTAAGGACGAGAGCCGCCCTGGGCTCTGCGCTGCATGTGCAGAAGTGCAA
AACAAAGTACATTGCGCTCGAAGGGCGGATCCGCCACCATGCCGGCCCAC
ATGCTCCAAGAGATCTCCAGTTCCTACACGACCACCACTACCATCACAGA
GCCTCCTTCCGGAAACCTGCAGAATGGACGAGAGAAGGCGAATTCTGCAG
ATCTAGCGCTACCGGTCGCCACCATGCCCGCCATGAAGATCGAGTGCCGC
ATCACCGGCACCCTGAACGGCGTGGAGTTCGAGCTGGTGGGCGGCGGAGA
GGGCACCCCCGAGCAGGGCCGCATGACCAACAAGATGAAGAGCACCAAAG
GCGCCCTGACCTTCAGCCCCTACCTGCTGAGCCACGTGATGGGCTACGGC
TTCTACCACTTCGGCACCTACCCCAGCGGCTACGAGAACCCCTTCCTGCA
CGCCATCAACAACGGCGGCTACACCAACACCCGCATCGAGAAGTACGAGG
ACGGCGGCGTGCTGCACGTGAGCTTCAGCTACCGCTACGAGGCCGGCCGC
GTGATCGGCGACTTCAAGGTGGTGGGCACCGGCTTCCCCGAGGACAGCGT
GATCTTCACCGACAAGATCATCCGCAGCAACGCCACCGTGGAGCACCTGC
ACCCCATGGGCGATAACGTGCTGGTGGGCAGCTTCGCCCGCACCTTCAGC
CTGCGCGACGGCGGCTACTACAGCTTCGTGGTGGACAGCCACATGCACTT
CAAGAGCGCCATCCACCCCAGCATCCTGCAGAACGGGGGCCCCATGTTCG
CCTTCCGCCGCGTGGAGGAGCTGCACAGCAACACCGAGCTGGGCATCGTG
GAGTACCAGCACGCCTTCAAGACCCCCATCGCCTTCGCCAGATCTCGATC
CAGCACAGTGGCGGCCGCTCGAGTCTAGAGGGCCCGTTTAAACCCGCTGA
TCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTC
CCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCT
AATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATT
CTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAA
TAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAA
GAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCA
TTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGC
CAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCA
CGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGG
TTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGG
TGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTT
TGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGA
ACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTT
GCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTA
ACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCC
CAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCA
GCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCA
AAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGC
CCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGC
TGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGA
GCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCA
AAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAGCACGTG
TTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAG
GTGAGGAACTAAACCATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCAC
CGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGT
TCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGAC
GTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACAC
CCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGT
CGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACC
GAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGC
CGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGACACGTGCTAC
GAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATC
GTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCT
GGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACA
AATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTG
CATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTG
TATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGT
TTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCC
GGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCAC
ATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGT
GCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGT
ATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGT
TCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAG
CAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAG
GCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGC
TCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTC
CGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCAC
GAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCT
TGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTG
AAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTG
CGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAG
ATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTAC
GGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGA
AGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTA
CCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTT
CATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAG
GGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTC
ACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGC
GCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGT
TGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGT
TGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGG
CTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCC
ATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAG
AAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATA
ATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAG
TACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTC
TTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAA
AAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATC
TTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTG
ATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAG
GAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGA
ATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTA
TTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAA
TAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC

Amino Acid Sequence

MPAHMLQEISSSYTTTTTITEPPSGNLQNGREKANSADLALPVATMPAMK
IECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFA
RTFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTE
LGIVEYQHAFKTPIAFARSRSSTVAAARV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11810
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Requested by Jeff Torestky. Component materials supplied by Jeff Torestky.; AW promoter sequence differs from provided reference sequence by one nucleotide (lacks t810 compered with reference) – this derives from pAW AMAXA GFP and matches C110076.4 GI:19909443 and XM_001144521.1 GI:114595088 and others.