cDNA Clone Nemo (mouse)

• Plasmid: pBS-CMV-Puro/Neo-IRES/TK Nemo targeting vector (SD/arm2/Mut/arm1)
• Parent Plasmid: pGEX6P3
• Genbank: NM_003071.3*
• Species: Human

Sequence of Insert

GTCGACATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCA
GACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATCCAA
CTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGACTTT
CTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGG
TCATGTGGTTGGACTACGCTATTACACGGGAGTAGTTAATAATAATGAAA
TGGTTGCATTACAACGAGATCCTAATAACCCTTATGATAAGAATGCAATT
AAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCT
TGCAGGTGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAG
GGGTAGTTCCTTTTGGTGCAAACAATGCTTTTACCATGCCTCTGCATATG
ACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGTTGAA
GAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATT
TGGAAAGTGGTTGGGGCTCTGGAAGAGCTGGACCAAGCTATAGTATGCCA
GTGCATGCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATTTGA
CAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAAC
CAGCTGAGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTA
GCTTGGATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTGGGA
ACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAGAAGG
ACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTG
GGTAAAACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGATGG
CAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGGAAT
ATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAA
AAGGCAGATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAG
TATTTCAGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGTCTA
GCTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGCAGT
GATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAA
ACTGAAAAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGGATCTTCTA
AGGTTATAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTTCCT
ACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTC
AAAGAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTC
CGCTTTCTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATAAAA
TCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTATTAG
AGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATA
TTTTAACTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGCATA
AGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCCAAA
TGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGG
TTTTGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTT
CTTTCCTTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCATAG
AACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGCGTT
TACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAA
ATTAAAGGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCA
GCACATTACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGAAAA
ATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTG
GCACATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTG
TTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCTCAG
GAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATGAAG
TTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTC
TTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAACCCT
GTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCTTTA
TGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGA
ATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTT
CAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAGAAT
CCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTCTTT
AATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATG
GTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAAC
ACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGGTGG
AGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAG
CCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTT
GGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTCTGT
TGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAGCAG
GAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAA
ATTAATGAAATCAGAACATTAATTGACTTACACCATCACCATCACCATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMSWMFKRDPVWKY
LQTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLTSDEEVDSVLFGSL
RGHVVGLRYYTGVVNNNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKK
ELAGALAYIMDNKLAQIEGVVPFGANNAFTMPLHMTFWGKEENRKAVSDQ
LKKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQLKTE
FDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPF
WEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFH
DGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKADGLSKDASRCSEQ
PSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMK
GKLKNVQSETKGRAKGSSKVIEDVAFACALTSSVPTTKKKMLKKGACAVE
GSKKTDVEERPRTTLIICPLSVLSNWIDQFGQHIKSDVHLNFYVYYGPDR
IREPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRN
PNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWW
HRTIQRPVTMGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVF
IQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAHYADVLGLLLRLRQ
ICCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAICL
DSLTVPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHEDNLLECPP
EELARDSEKKSDMEWTSSSKINALMHALTDLRKKNPNIKSLVVSQFTTFL
SLIEIPLKASGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLKA
GGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKD
SVEENMLKIQNKKRELAAGAFGTKKPNADEMKQAKINEIRTLIDLHHHHHH*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11607
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion and C-ter 6His.; Sequence differences compared with NM_003071.3 CDS:; g909a (sil T303) ; delta agc nt1284-1286 (delta residue A429*) ; *derives from EST -also deleted in some published seqs.; ; Estimated MW of expressed protein : 142169 Daltons