cDNA Clone DPB3 (yeast)

• Plasmid: pRS415 Pro-DPB3-6His-cMyc
• Parent Plasmid: pRS415
• Genbank: DQ331917.1 GI:84312180
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCACGAGGATCTACTTTCAGAAATGGTAAAATGCTATGATTGTGAC
AATAAGTAGCAGCGTTTTCCAAATCAACATCATCCACTTGCAATTCCAAT
TGTCGTATCTGAAACTTTAGCAAGTTCAAAAGTTCCTTCGTGTTATTGGG
ATATTGAAAACCATTAGAATCATTTCCAGTTTCACAGGTATCGCCCTCTC
TATGAAAGGAAGTGCGAACTTTCCAGGACTGGAAGATATCATTCACTTCG
AGAGAATTACGCAAAAGCGCAGAATTGTTAGCATCTGATTGCGATTTGAC
TATTGCAAGAGAATGAGGAAAATCGGGTGGTTGACCAAAGGAAATATCAT
GAGCCCAGGGGAACATTTTTTTACATTCAGGCCATTCTGCATCCTGATGT
AGTCGCATAAGATCATGCAGTTCACCAGGTGTGATTGAGGGCACTTCCAA
AACCATGGTTGTTGCTCTAGTTTTCTATACCCGCCCCTTCTACCTCAATG
ACAATCTCTCTTTTATGGTTGGTGTTTCTTTTCACTTTTTTACTTCTTCT
TGTACTTTTCTTTTTGAACCTTAAAAACTCTTGCAATTATGTTTCCATGA
TGCTTTTGAAGTAATAATTTATTTTTATTACGCGAAATTAATGGCCTTAA
CAACTGCCTGTCCCAACTGATAAAAACAAGCAAGGGTCAACCGTGTTGCA
AAAAAAATGTCCAATTTAGTTAAAGAAAAAGCACCTGTCTTTCCTATATC
TAAAGTAAAGAAGATTGCCAAATGCGATCCCGAATACGTAATTACATCTA
ATGTAGCTATATCAGCGACCGCATTCGCTGCTGAGTTATTTGTACAGAAT
CTCGTCGAAGAATCGCTGGTCTTAGCACAACTGAATTCGAAAGGAAAGAC
AAGCCTACGATTAAGCCTAAATTCTATAGAAGAATGTGTCGAAAAAAGAG
ATAATTTCAGGTTTCTAGAGGATGCCATTAAACAACTGAAGAAGAATAGC
GCACTCGACAAAAAGAGAGAACTAAACATGCAACCGGGTCGGAGCGATCA
AGAGGTTGTTATAGAAGAGCCTGAATTGCATGAGGATGATGGTGTAGAGG
AAGAAGAAGAAGAAGACGAGGTATCCGAAGAAGAAGAGCCCGTACACAAT
GAAGAACTTCTTGATGATAGTAAAGATCAGCAAAATGATAAATCCACGCG
CAGTGTGGCAAGCTTGCTGTCGAGATTCCAGTATAAATCCGCACTAGACG
TAGGAGAACATTCCGACTCTTCTGATATCGAAGTTGACCATACGAAAAGC
ACCGATCCTCACCATCACCATCACCACGGTGAACAAAAGCTAATCTCCGA
GGAAGACTTGTAGGCGGCCGC

Amino Acid Sequence

MSNLVKEKAPVFPISKVKKIAKCDPEYVITSNVAISATAFAAELFVQNLV
EESLVLAQLNSKGKTSLRLSLNSIEECVEKRDNFRFLEDAIKQLKKNSAL
DKKRELNMQPGRSDQEVVIEEPELHEDDGVEEEEEEDEVSEEEEPVHNEE
LLDDSKDQQNDKSTRSVASLLSRFQYKSALDVGEHSDSSDIEVDHTKSTD
PHHHHHHGEQKLISEEDL*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11556
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences in promoter sequence compared with ref:; g68t, t133c, c655t, a662t; Sequence differences compared with DQ331917 CDS:; c9t, c72t, g306a, a474g, c555t (all silent)