cDNA Clone DPB4 (yeast)

• Plasmid: pGEX6P-DPB4
• Parent Plasmid: pGEX6P1
• Genbank: AY557673.1 GI:45269238
• Species: Yeast

Sequence of Insert

GGATCCATGCCACCAAAAGGTTGGAGAAAAGACGCCCAAGGGAATTACCC
CACTACTTCGTATATTAAAGAACAAGAGAACATTACGATTCAGGACCTGT
TATTTCCTAAAAGCACTATTGTGAATTTGGCACGCGAAGTGCCACAGCAA
AGCGGCAAAAAGCTGCTGATAAATAAAGACGCATCACTAGCGTTACAGCG
TGGTGCTACAGTATTTGTTAACCACTTGCTCCTGTTTGCAAGGGAAATTG
CAAAGAGTCAAGATAAAAAAAGCTGCAGTGTAGATGATGTTCTGAGTGCT
TTGGACCATATAGGACACTCGGCATTGAAGGGTCCGGTTAGAGATAAACT
AGATGAATACCAAGCTGCTGTTGAACAGAGGAAGAAGGAAAAACTAGATA
GTGGTGAAGTAGATGCTGATGGCGATATAGATATGGGTGAAGATAAGGAG
AATGTGCCCGTGGAAAAGGTTAAAGAGCATGACGAAATAGAAGAACAAGG
CGATGCATTACAGGACGTAGAAGAGTCCTCCGAAAAGAAACAGAAAACGG
AGAGCCAAGATGTAGAAACTAGAGTTCAAAACCTTGAGCAAACGTAAGCG
GCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPPKGWRKDAQGNYPTTSY
IKEQENITIQDLLFPKSTIVNLAREVPQQSGKKLLINKDASLALQRGATV
FVNHLLLFAREIAKSQDKKSCSVDDVLSALDHIGHSALKGPVRDKLDEYQ
AAVEQRKKEKLDSGEVDADGDIDMGEDKENVPVEKVKEHDEIEEQGDALQ
DVEESSEKKQKTESQDVETRVQNLEQT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11553
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 48820 Daltons