cDNA Clone DPB3 (yeast)

• Plasmid: pGEX6P-DPB3
• Parent Plasmid: pGEX6P1
• Genbank: DQ331917.1 GI:84312180
• Species: Yeast

Sequence of Insert

GGATCCATGTCCAACTTAGTTAAAGAAAAAGCACCTGTCTTTCCTATATC
TAAAGTAAAGAAGATTGCCAAATGCGATCCCGAATACGTAATTACATCTA
ATGTAGCTATATCAGCGACCGCATTCGCTGCTGAGTTATTTGTACAGAAT
CTCGTCGAAGAATCGCTGGTCTTAGCACAACTGAATTCGAAAGGAAAGAC
AAGCCTACGATTAAGCCTAAATTCTATAGAAGAATGTGTCGAAAAAAGAG
ATAATTTCAGGTTTCTAGAGGATGCCATTAAACAACTGAAGAAGAATAGC
GCACTCGACAAAAAGAGAGAACTAAACATGCAACCGGGTCGGAGCGATCA
AGAGGTTGTTATAGAAGAGCCTGAATTGCATGAGGATGATGGTGTAGAGG
AAGAAGAAGAAGAAGACGAGGTATCCGAAGAAGAAGAGCCCGTACACAAT
GAAGAACTTCTTGATGATAGTAAAGATCAGCAAAATGATAAATCCACGCG
CAGTGTGGCAAGCTTGCTGTCGAGATTCCAGTATAAATCCGCACTAGACG
TAGGAGAACATTCCGACTCTTCTGATATCGAAGTTGACCATACGAAAAGC
ACCGATCCTTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSNLVKEKAPVFPISKVKK
IAKCDPEYVITSNVAISATAFAAELFVQNLVEESLVLAQLNSKGKTSLRL
SLNSIEECVEKRDNFRFLEDAIKQLKKNSALDKKRELNMQPGRSDQEVVI
EEPELHEDDGVEEEEEEDEVSEEEEPVHNEELLDDSKDQQNDKSTRSVAS
LLSRFQYKSALDVGEHSDSSDIEVDHTKSTDP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11552
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Differences compared with DQ331917.1 CDS:; c72t, g306a, a474g, c555t – all silent; ; Estimated MW of expressed protein : 49487 Daltons