cDNA Clone DPB2 (yeast)

• Plasmid: pGEX6P-DPB2
• Parent Plasmid: pGEX6P1
• Genbank: AAB68109
• Species: Yeast

Sequence of Insert

GGATCCATGTTTGGCTCTGGGAATGTTCTGCCTGTTAAAATTCAGCCTCC
GCTGCTTAGGCCACTGGCGTATAGAGTTTTATCAAGAAAATATGGTTTAT
CAATTAAATCTGATGGACTGTCTGCTCTTGCAGAATTCGTTGGCACTAAT
ATAGGTGCTAACTGGAGACAAGGGCCTGCTACAATAAAATTTCTCGAGCA
ATTTGCTGCAGTATGGAAACAACAAGAAAGAGGCCTTTTCATCGACCAGA
GCGGGGTTAAAGAAGTTATACAGGAGATGAAAGAACGTGAGAAAGTCGAA
TGGAGTCATGAACACCCTATTCAACATGAGGAGAATATCTTGGGACGGAC
AGATGACGATGAGAACAACAGTGACGATGAAATGCCGATCGCTGCTGATT
CATCCTTACAAAATGTTTCATTATCTTCACCCATGCGACAGCCCACCGAA
AGAGATGAATATAAACAGCCTTTCAAGCCAGAAAGCTCCAAGGCGTTGGA
CTGGAGGGACTACTTCAAAGTTATCAATGCTTCTCAGCAACAAAGATTCT
CATACAATCCGCACAAAATGCAATTTATTTTCGTCCCCAATAAAAAGCAG
AATGGGCTAGGAGGCATTGCGGGTTTTCTACCAGATATAGAGGACAAAGT
TCAAATGTTCTTGACAAGATATTACCTCACGAACGACAGAGTCATGAGAA
ATGAAAACTTCCAAAACAGTGACATGTTTAATCCATTATCATCTATGGTG
TCTTTACAAAATGAACTGTCCAATACTAACCGACAGCAGCAATCCAGCAG
TATGAGTATCACCCCAATAAAAAACCTACTAGGTAGGGATGCTCAAAACT
TTCTACTATTGGGGCTCTTAAATAAGAACTTTAAGGGCAATTGGTCACTG
GAAGATCCGTCTGGATCTGTCGAAATTGACATTTCCCAAACTATCCCTAC
ACAAGGCCATTACTACGTGCCAGGTTGCATGGTTCTTGTGGAAGGAATAT
ACTATTCTGTAGGAAATAAATTCCATGTTACCTCCATGACTTTACCCCCT
GGTGAGAGAAGAGAAATTACATTAGAAACGATAGGTAATCTCGATCTCTT
AGGAATACATGGCATTTCTAATAACAATTTCATTGCTCGTTTGGATAAAG
ATTTGAAGATTAGATTACACCTTTTGGAGAAAGAATTAACAGATCATAAA
TTTGTAATTCTCGGCGCAAATTTGTTCCTAGATGATTTGAAAATTATGAC
TGCACTCAGCAAAATTTTGCAAAAATTAAATGATGACCCACCGACCCTAT
TAATTTGGCAAGGTTCTTTCACTTCAGTTCCCGTTTTCGCATCAATGAGT
AGCCGGAATATAAGTAGTTCCACTCAATTTAAGAATAATTTCGATGCCTT
GGCGACGCTTCTGTCAAGATTTGACAATTTGACCGAAAATACCACAATGA
TATTTATTCCAGGTCCTAACGATTTATGGGGGTCGATGGTGTCATTGGGG
GCAAGTGGGACATTACCGCAAGATCCAATTCCTAGTGCGTTTACCAAAAA
AATCAACAAGGTCTGTAAAAACGTTGTATGGAGCTCAAATCCAACTAGAA
TAGCATACTTATCCCAAGAAATAGTCATTTTCAGGGACGATTTATCCGGA
AGATTCAAAAGACACCGTTTGGAATTCCCATTCAACGAGAGCGAAGATGT
TTATACTGAAAACGATAACATGATGTCTAAAGACACCGATATTGTACCAA
TCGATGAATTAGTTAAAGAACCAGACCAGTTACCACAAAAGGTTCAAGAA
ACAAGAAAACTCGTTAAAACAATACTAGACCAGGGTCATTTATCGCCATT
TCTTGATTCCTTGCGCCCAATTTCATGGGATTTGGACCACACTTTGACAC
TTTGCCCAATACCATCGACAATGGTTCTTTGCGACACTACTTCTGCACAA
TTTGATTTGACATACAACGGTTGTAAAGTAATTAACCCTGGAAGTTTCAT
TCATAATAGACGTGCTAGGTATATGGAGTACGTTCCATCCTCAAAGAAAA
CTATACAAGAAGAGATATATATCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMFGSGNVLPVKIQPPLLRP
LAYRVLSRKYGLSIKSDGLSALAEFVGTNIGANWRQGPATIKFLEQFAAV
WKQQERGLFIDQSGVKEVIQEMKEREKVEWSHEHPIQHEENILGRTDDDE
NNSDDEMPIAADSSLQNVSLSSPMRQPTERDEYKQPFKPESSKALDWRDY
FKVINASQQQRFSYNPHKMQFIFVPNKKQNGLGGIAGFLPDIEDKVQMFL
TRYYLTNDRVMRNENFQNSDMFNPLSSMVSLQNELSNTNRQQQSSSMSIT
PIKNLLGRDAQNFLLLGLLNKNFKGNWSLEDPSGSVEIDISQTIPTQGHY
YVPGCMVLVEGIYYSVGNKFHVTSMTLPPGERREITLETIGNLDLLGIHG
ISNNNFIARLDKDLKIRLHLLEKELTDHKFVILGANLFLDDLKIMTALSK
ILQKLNDDPPTLLIWQGSFTSVPVFASMSSRNISSSTQFKNNFDALATLL
SRFDNLTENTTMIFIPGPNDLWGSMVSLGASGTLPQDPIPSAFTKKINKV
CKNVVWSSNPTRIAYLSQEIVIFRDDLSGRFKRHRLEFPFNESEDVYTEN
DNMMSKDTDIVPIDELVKEPDQLPQKVQETRKLVKTILDQGHLSPFLDSL
RPISWDLDHTLTLCPIPSTMVLCDTTSAQFDLTYNGCKVINPGSFIHNRR
ARYMEYVPSSKKTIQEEIYI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11551
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Differences from AAB68109 sequence:; F458Y, T645P.; ; Estimated MW of expressed protein : 105162 Daltons