cDNA Clone GRIPE

• Plasmid: pCMV5-HA GRIPE H1147-A2034
• Parent Plasmid: pCMV5 HA1
• Expressed: HA GRIPE H1147-A2034
• Genbank: NM_194301.2 GI:51230411
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCcatgcatataaacttatttgtaatacaatgaaaagaagacaagatgtttctccaaatagagattttctaacacatttctacaatataatgcattgtggattacttcatattgaccaggatattgtcaatacaatcatcaaacactgctcacctcaatttttttcacttggtttgcctggtgccacaatgcttattatggattttattgtagcagctggtagagtggcttcttcagcttttctcaatgcaccaagagtagaagcacaagttcttctgggatctttggtttgctttcccaac
ttatattgtgaactgccttctcttcatcccaacattcctgatgttgctgt
gtctcagtttacagatgttaaggaacttataatcaaaactgtattaagct
cggcaagagatgagccctctggtcctgcacgatgtgtagcactttgtagt
ttaggtatttggatttgtgaagaactagtccatgagtctcatcatcctca
aattaaggaagctctgaatgtgatttgtgtttccttaaagtttactaata
aaacagtagcccacgtagcttgtaacatgcttcacatgctggttcattat
gtacctagacttcagatttaccagcctgattctcccttgaaaattattca
aatcctaatagctaccatcacccatcttttaccaagtacagaggcttcat
cttatgaaatggacaagaggttggtagtatctttacttctctgccttctg
gactggatcatggccttacctctaaagacactgctccaaccatttcatgc
tacgggagcagaaagcgataaaacagaaaaatctgttctcaattgcattt
ataaggttttacatgggtgtgtttatggagctcagtgttttagcaatcca
aggtattttcccatgagcctctctgatttggcatctgtagattatgatcc
ttttatgcatttggaaagtctgaaagagcctgagcctctgcactctcctg
actcagaacgatcttctaaactccagccagtaacagaagtgaaaactcaa
atgcagcatggattaatctctatagcagcccgcactgttattacacatct
ggtaaatcacctgggccattatccaatgagcggtggtcctgctatgctaa
caagtcaggtgtgtgaaaatcacgacaatcattacagtgaaagtactgaa
ctttctcctgaactctttgagagtccaaatatccagttctttgtgttaaa
taatacaaccttagtgtcctgtatccagatcagatcagaagagaatatgc
ctggaggaggtttatctgctggccttgcatcagccaattcaaatgtcaga
atcatagtacgtgatctctctggaaaatattcatgggattctgctatact
gtatggcccacctcctgtaagtggcttgtcagaacctacatctttcatgc
tttcattgtctcaccaagagaagccagaagagcctccgacatctaatgaa
tgcttagaagatataaccgtaaaagatggactttctctccagtttaaaag
atttagagaaactgtaccaacttgggatacaataagagatgaagaagatg
ttcttgatgagctcttgcagtatttgggtgttactagtcctgaatgctta
cagagaactggaatctcacttaatattcctgctccacaacctgtgtgcat
ttctgaaaaacaagaaaatgatgttattaatgctatccttaagcaacata
cagaagaaaaagaatttgttgagaagcactttaatgacttaaacatgaaa
gctgtggaacaagatgaaccaatacctcaaaaacctcagtcagcatttta
ttattgcagattgcttcttagtatattgggaatgaattcctgggacaaac
ggaggagctttcatctcctgaagaaaaatgaaaagctacttagagaactt
aggaacttggattcaaggcagtgccgagagacacacaagattgcagtatt
ttatgttgctgaaggacaagaagacaaacactccattctcaccaatacag
gaggaagtcaagcatatgaagattttgtagctggtcttggttgggaggta
aatcttacaaaccattgtggttttatgggaggactacaaaaaaacaaaag
cactggattgaccactccatattttgctacctctacagtagaggtaatat
ttcacgtgtcaacaagaatgccttctgattctgatgattctttgaccaaa
aaattgagacatttgggaaatgatgaagtgcacattgtttggtcagagca
tactagagactacaggagaggaattattcccacagaatttggtgatgtcc
ttattgtaatatatccaatgaaaaatcacatgttcagtattcagataatg
aaaaaaccagaggttcccttctttggtcccctttttgatggtgctattgt
gaatggaaaggttctacccattatggttagagcaacagctataaatgcaa
gccgtgctctgaaatctctgattccattgtatcaaaacttctatgaggag
agagcacgatacctgcaaacaattgtccagcaccacttagaaccaacaac
atttgaagattttgcagcacaggttttttctccagctccctaccaccatt
taccatctgatgccgATCATTAAATATCAGTTCTGTTTATCTGAAGgctc
ctacccagagattctacccagtgaaactcccacagcaacgcaggtagatg
gggctgacctggcctctccaatgtctcctcgaactagcaaaagccgcatg
tccatgaagctgcgtcgttcctctggctcagccaataaatcctaaGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHID
QDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEA
QVLLGSLVCFPNLYCELPSLHPNIPDVAVSQFTDVKELIIKTVLSSARDE
PSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAH
VACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMD
KRLVVSLLLCLLDWIMALPLKTLLQPFHATGAESDKTEKSVLNCIYKVLH
GCVYGAQCFSNPRYFPMSLSDLASVDYDPFMHLESLKEPEPLHSPDSERS
SKLQPVTEVKTQMQHGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVC
ENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRSEENMPGGGL
SAGLASANSNVRIIVRDLSGKYSWDSAILYGPPPVSGLSEPTSFMLSLSH
QEKPEEPPTSNECLEDITVKDGLSLQFKRFRETVPTWDTIRDEEDVLDEL
LQYLGVTSPECLQRTGISLNIPAPQPVCISEKQENDVINAILKQHTEEKE
FVEKHFNDLNMKAVEQDEPIPQKPQSAFYYCRLLLSILGMNSWDKRRSFH
LLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQA
YEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVST
RMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIY
PMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALK
SLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDA
DH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11516
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Intention to clone H1147-S2083(end) but insertion in EST clone 5527017 introduces stop codon after residue 2034.; ; Estimated MW of expressed protein : 101516 Daltons