cDNA Clone SMARCA3

• Plasmid: pGEX6P-3-SMARCA3 H700-end
• Parent Plasmid: pGEX6P3
• Expressed: GST-SMARCA3 H700-end
• Genbank: NM_003071.3
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACCATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAAT
TTGTTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCT
CAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATG
AAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGA
TTCTTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAAC
CCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCT
TTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGA
AGAATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCA
GTTCAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAG
AATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTC
TTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGG
ATGGTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAA
AACACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGG
TGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATC
CAGCCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGA
CTTGGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTC
TGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAG
CAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCC
AAAATTAATGAAATCAGAACATTAATTGACTTATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDHYADVLGLLLRLR
QICCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAIC
LDSLTVPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHEDNLLECP
PEELARDSEKKSDMEWTSSSKINALMHALTDLRKKNPNIKSLVVSQFTTF
LSLIEIPLKASGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLK
AGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVK
DSVEENMLKIQNKKRELAAGAFGTKKPNADEMKQAKINEIRTLIDL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11473
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 61955 Daltons; ; All SMARCA3 plasmids MUST be grown at 30 degrees C or less to prevent recombination.