cDNA Clone Mms22 (yeast)

• Plasmid: pRS415 Pro-15HA-Mms22
• Parent Plasmid: pRS415
• Genbank: YSCL8543
• Tag: 15HA
• Species: Yeast

Sequence of Insert

GGATCCATTGCTTGTTTAACCTTTAGTTTGTCTAAAAGTGTCACTATAAT
CTCTCTGATTTTTGGCAGATGTTTATTTAAAGTTTCATCAGATGGTGGTT
CCCTCAATGTCACTTCCAATACACGTCTTAAAGCCATTGGCACATCACCA
ACATCTGACATGTCAACTTTCGCATGCATGAAGAATTTCGATACGACTTT
GAAGTCATTGCCCAACTGAACGTATGCATCAGATACTAATCTTCCAGAGG
TTGTCCCCTTAGACCATTGTGTTAAGACCTGGAGGAGGTGCTTGGTGGAC
ATCAGCAACTTTGTTACTGTCGTTTCAATACTACTACTGCTAGAAGCAGT
TCTTTTAATCTTTGGGGTGCCATACGTAGGGTCATCCACGGCCATCTTCA
TCTAATTTTAAAATAATACGAGGATTACTACACCAATTTGGCAGCCAGCT
TCTACTCTTTTTCCCTTTTCTTGGGTTTGTTTTGATGCCATTATCTCAAG
AATGCATAATAGTAGGATAAAATTCGTTCAGCTCGGCTAGCGAGATATCT
TCAAAGAGTAAAGCGCATCTGCGTGTAAAATTAAATTCAAGAAGTGAAAG
AATAAAAGATAGGATTGTAGATTTGAAGTTTAGTGGTAGCGTCAGCAGAT
AAGCCCATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTCAGTGCGGCCGCATCTTTTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCA
GATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTA
TCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAG
ATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTAT
CCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGA
TTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATC
CCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGAT
TACGCTGCCATGGATGTTGATGAACCGAATCCTATTGTGATATCAGATTCTGAGGCCACTGATGAGGAAATTAGTATCATATACGAACCCGAATTCAATGAAAATTATTTATGGGCAGAGGAAAACGTCCAAGAAGCAAGCAGATCACAGAAA
ATTGTCACTGAGAGGTTGAGCCTGGATTCCACTGCTGGAGAATCTTGTAC
TCCTTCCGTAGTAACTGATACACAAGTTACTACGGGGCTTCGGTGGTCTT
TGAGGAAGAGGAAGGCTATTCAAAAGATGCCATACAGTTTAGAACGCATA
AAGCATAGGCAGTTGCTAGAGGGCTATGATATCTCTAGTTTTGACAGCAT
TTCGAATCAGTTGACCTTACCGAAGAACGCTTCTACAGTGATTCACTCAA
ATGATATTTTACTTACAAAGAGAACTGGTAAACCCTTAGATGAGCAGAAA
GATGTTACAATCGACTCCATTAAACCCGAAAACAGCTCGGTTCAATCTCA
AAGATATGATAGTGATGAAGAAATACCAAAGAAAAGGCATCGTACTTTTA
AAGACCTCGACCAGGATATAGTATTTCAATCAGGTGATTCTACGGAAGAT
GAACAAGACTTGGCTAGTACAAATTTACAGAATACTCAAAATGACGAAGT
AATATTTAGAGGAAGAGTATTGAATGTAAGAACTGGTTACCGTGGGGTCC
TGCCAAGAGTTGCTTGGGAAAAGTCGTTGCAAAAGCAGCAGTCTTCAAAA
GTCACGAAAAGAAAAACTCAATTATTGAACCATAAGGGTGTGGCTAAGAG
GAAAATGAACAGAAGCGCCCATATTGAAGATGAAGAACAGAACTTATTGA
ACGATCTTATTGCACCTGATGACGAACTGGATATCGAGGAAAACGCCCCT
CCAGATATATACTTAGGAAATCTTCCAGAGGATCGTGAGGCTAACGAAAA
GGAATTAAAGGAATTGCAAGAATATTATGAGAGCAAGTATAGTGAAGACG
CACAATCGGCTGGAACTTCTGGTTTTAATTTAAATGAGGAATACCGCAAT
GAACCAGTGTATGAGCTGGAATATGACGGACCGGGGAGCTGCATATCACA
TGTGTCTTATAAAGACCAGCCTATTATTTATTTGAATTCACGGCATTCAG
ATAGCGGATTTAGTGAGCAATACAATATATCTGCCGAAGATAATCAAAGT
GTAATTTCCCTAGATGCTGCTGAAGAGCATAACGATGGTATAATTGACAA
GATGTTGGTTAAACCTAAAAGAATTAAAGCAACCAATGATGCCAACTTTT
TGAATACAAAAAGTAAGAGAGTGAGAAGATATAAGTACAAATATCGAAAT
TCTTGTCTGGCCCCAAGTACAAAAGCTATCAAAGTCGGAAAACGGTCAGC
GCATAAATCTCATTTGGCAGCTAATAATCCAGTTTCATTTGTTTCTAAGA
AGAATCATGTAATTGATGATTATTTTTTTGAAGAATTAGAATCTCAATCC
CTAGAACAGGATGACAGCTCTTCTCTCAAGCCACAGAAAAAAAGAAGGAA
GAAGAAAGCACCTATATACTCAAGTTTCTCCGCTGATCTTGAATCGAGAC
GAAAACCAGTTTTCAATACTGTTGTAGAGGTTCCCACGAACCGATACGCT
TTCACAAAGCCAAATGTACGTAATAGGGACAGCATTAATCATGACATGGA
ATTTGAGGAAGAAGACTCCAATCAAGAGCTTGGACCAATAATGGTCGTAC
TTGACTCAATTTTACTAAAAAAACCTTTTGAGCCACCTAATTTCTTCAAG
ATCCAGTTGTCAGACAAGAGTTTCTTGCTATCAAAATTGAATCCTGCAGA
TATAGCTACATCATTACAGAAAATATTCAGAGTAATCATTGATAAAGGTA
TCACTGATACTGAACTAGTTCACTTTAACGAAAGCCTTATTGCTTTTTTG
GTGCACCTTGACATGCCTGAACTATTCGACTTAATAGGTGAATTTCATCG
TGAATTTAGATCGAAGGTCAATAGCTTGAGGAAAAAAGCTAAACCAATCC
ATTTTTTTCAAATTGCGGCATGCCAATTAATGTTTCTAGAAATATCAAGA
TATAACAAAATTTCAGCCGCTGCAAAATTCGACATGGATGTCAAACTCTT
AGATCATATTGTGTCTTTCTTCAAATTACTTTCTGTCTGTTATGATTCTG
TTATGAAAAATCCAATGCAATATCTGTACACAAGTTATTATATTCTCTCT
GCTGTCGTTGATGTCATACATAAAAAGGAAGCTTTGTGGGATTTATTTCA
AAAGCACCCCTTTTCGCCTCATATTTCACTGTTGCTGGTCAACATATTTC
CTACGAAAGTCTGCCGCTGGCAGGTTTTGAGATTAGATTCTGAATTCCAA
CCCTTGAGTTCGGCATTTCGGTTCATCAATTATTGCATTGAGACTTGTAA
TTGGAATGTTACAAATTCTTTGATTCTTTCTCTCGATCGCATATTCAAAC
GGAGAAGATTTTCGGACTTTGAAGAAGAGTCTGATTTGTCACAAAACAAT
AAGATAATTTATCCTCCAACGAATCAGCTTACGAGTCGATTAATGTTTAA
CAGATATTTGCATCTACTGACGTTGTGTGAATTGTCTAGTTCTGATACAC
AACGTGTGATACCGATGGGTGATATTTCTATGAATGATTCTCTATCTGTT
TTGAAGAACCGCCTCAACCTACTAATTGTTCTAGCTACACGTTTTGATTT
AAACTTAGAGAAGCGCTTCCAAGAGCTAACACGACCGTTATATAGTAAGG
AATATTTAAACTTGCATACACAGAACACAGTCAGGACGATAACAACATTG
ATCATGCAGGCCAGCCTTTCATTCCTGGAAATCAGTCGAATCAAAAATCA
TCCATTCAGTGGAAAATTTATTGCGTCATTATTTGATAAACTAGTACTTC
AACAACCTTCCATATCGGGAGTAACAGAAAATTTTTTGAAAGAATTCACA
AATTTAGTCTCCAAAATGAAAAGAAAAAGCGTGAGTATGCTCAAATTTCT
GTATCCTTCACTGGTGGCCATGTCTCAAGAAAATATATTCGAGTCGTCTT
TTTTTTTATTATTACAGGTATATTTGAAGAGCTTAGATGTGCTGGGTCCA
ACTTGGGTGCAAAATTATCTTTTTCAGTTTATAAAGAGCAAGGCTCAAGA
AAATGAAAGATGGATAGAATGCTATTGTCAGATCGGAAAATTCCTCGTAG
ATTCTGGTATATTTACCTGGTGGACTTTCTTTACCTATAACGGCTTAGAT
GCTGCATTACACTTTCAGCTTGCATTTCACTCCCTTATTATCGATTTCTG
TGATACAGACTCTTTTGAATTACTGAAAAAACCGCTTTATTCCATTGCTT
CTGATTTGCTGCTCATTTCCAAAGATGACGCTTTCTATCACTTTCTTTCC
AATCTGTTGAAGAGGGCACATATCATAGTAGCAGATTTGAAACCTGTTTC
CGATGAGAATGAACTCCTGCGATTGGCTTATATTTTTTCTAAAGCATTGA
AAAAAAATGCATATCAAGATCTATTAGCTGTTTTCTTATCCCTGGCGAAA
AAACATTACGATGAAGGGGACATCAGTAGAAATTTCTTGGCAAAATATCT
CGAATTTCTTAACAAAAATTGCTTGACTGAGCTAAGAAATAATCAACTCT
TCATCAGTCTAAGAAGGGAACTTGGTATTTCAAGTGATGAAGACGAAAAA
TGTGCGTTCTGGGACTCATTCAATGAAGCAGGTGATATACTTTCCAAAGC
TGCATTTGTTGAAACCGGTATTGTTCAAGCCTGTTGCACTGGGAATGAAA
TTGATGGATATCTTGACAATTTAAGCACTTTATTCACCTCCACCATGTTA
GAGAGCCCTTTTGCATTTTTTTCTGACCTGGTGATAGCACACATATTTGA
GAACAGACCTTTTTTCGACGTGAATATCAAGAATTTTCTTCTATCTCACT
TCATAGACCTTTTCAACAAGGTTTTAAAAATGAAATTCGAGCAGGTATCT
CCTGATGAATTTGCTGAACTTTGCAAAGTATACAGGGCGTTATGTATTGA
GTGCGCAACAGACGACACCTTTAATAGTAATAGTGATTTGATCGCCGCAA
AAGATGCGTTTTTGGTATCTGTGTTGAGAATCGCAGATGGGTTTTGGGAG
CATGACAAACTTTTGCAGCTAAGGATGCTTGATAGTAATATGAACATACC
TAATCAGATTCCTCATACTACTTTGCAAAGTTCGCTATCTGCGATTGTAA
TAAAGATAATAGAAAGTAATATAGGAAAAATTGAGGCGTCTGAACCTTTC
AAAACCTTCAAGAATACGTAAGCGGCCGC

Amino Acid Sequence

MGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAQCGRIFYPYDVPDYAGY
PYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPD
YAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYP
YDVPDYAGSYPYDVPDYAAMDVDEPNPIVISDSEATDEEISIIYEPEFNE
NYLWAEENVQEASRSQKIVTERLSLDSTAGESCTPSVVTDTQVTTGLRWS
LRKRKAIQKMPYSLERIKHRQLLEGYDISSFDSISNQLTLPKNASTVIHS
NDILLTKRTGKPLDEQKDVTIDSIKPENSSVQSQRYDSDEEIPKKRHRTF
KDLDQDIVFQSGDSTEDEQDLASTNLQNTQNDEVIFRGRVLNVRTGYRGV
LPRVAWEKSLQKQQSSKVTKRKTQLLNHKGVAKRKMNRSAHIEDEEQNLL
NDLIAPDDELDIEENAPPDIYLGNLPEDREANEKELKELQEYYESKYSED
AQSAGTSGFNLNEEYRNEPVYELEYDGPGSCISHVSYKDQPIIYLNSRHS
DSGFSEQYNISAEDNQSVISLDAAEEHNDGIIDKMLVKPKRIKATNDANF
LNTKSKRVRRYKYKYRNSCLAPSTKAIKVGKRSAHKSHLAANNPVSFVSK
KNHVIDDYFFEELESQSLEQDDSSSLKPQKKRRKKKAPIYSSFSADLESR
RKPVFNTVVEVPTNRYAFTKPNVRNRDSINHDMEFEEEDSNQELGPIMVV
LDSILLKKPFEPPNFFKIQLSDKSFLLSKLNPADIATSLQKIFRVIIDKG
ITDTELVHFNESLIAFLVHLDMPELFDLIGEFHREFRSKVNSLRKKAKPI
HFFQIAACQLMFLEISRYNKISAAAKFDMDVKLLDHIVSFFKLLSVCYDS
VMKNPMQYLYTSYYILSAVVDVIHKKEALWDLFQKHPFSPHISLLLVNIF
PTKVCRWQVLRLDSEFQPLSSAFRFINYCIETCNWNVTNSLILSLDRIFK
RRRFSDFEEESDLSQNNKIIYPPTNQLTSRLMFNRYLHLLTLCELSSSDT
QRVIPMGDISMNDSLSVLKNRLNLLIVLATRFDLNLEKRFQELTRPLYSK
EYLNLHTQNTVRTITTLIMQASLSFLEISRIKNHPFSGKFIASLFDKLVL
QQPSISGVTENFLKEFTNLVSKMKRKSVSMLKFLYPSLVAMSQENIFESS
FFLLLQVYLKSLDVLGPTWVQNYLFQFIKSKAQENERWIECYCQIGKFLV
DSGIFTWWTFFTYNGLDAALHFQLAFHSLIIDFCDTDSFELLKKPLYSIA
SDLLLISKDDAFYHFLSNLLKRAHIIVADLKPVSDENELLRLAYIFSKAL
KKNAYQDLLAVFLSLAKKHYDEGDISRNFLAKYLEFLNKNCLTELRNNQL
FISLRRELGISSDEDEKCAFWDSFNEAGDILSKAAFVETGIVQACCTGNE
IDGYLDNLSTLFTSTMLESPFAFFSDLVIAHIFENRPFFDVNIKNFLLSH
FIDLFNKVLKMKFEQVSPDEFAELCKVYRALCIECATDDTFNSNSDLIAA
KDAFLVSVLRIADGFWEHDKLLQLRMLDSNMNIPNQIPHTTLQSSLSAIV
IKIIESNIGKIEASEPFKTFKNT*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11463
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid for 15HA-tagged Mms22.