cDNA Clone Mms22 (yeast)

• Plasmid: pRS413 Pro-13Myc-Mms22
• Parent Plasmid: pRS413
• Genbank: YSCL8543
• Tag: 13Myc
• Species: Yeast

Sequence of Insert

GGATCCATTGCTTGTTTAACCTTTAGTTTGTCTAAAAGTGTCACTATAAT
CTCTCTGATTTTTGGCAGATGTTTATTTAAAGTTTCATCAGATGGTGGTT
CCCTCAATGTCACTTCCAATACACGTCTTAAAGCCATTGGCACATCACCA
ACATCTGACATGTCAACTTTCGCATGCATGAAGAATTTCGATACGACTTT
GAAGTCATTGCCCAACTGAACGTATGCATCAGATACTAATCTTCCAGAGG
TTGTCCCCTTAGACCATTGTGTTAAGACCTGGAGGAGGTGCTTGGTGGAC
ATCAGCAACTTTGTTACTGTCGTTTCAATACTACTACTGCTAGAAGCAGT
TCTTTTAATCTTTGGGGTGCCATACGTAGGGTCATCCACGGCCATCTTCA
TCTAATTTTAAAATAATACGAGGATTACTACACCAATTTGGCAGCCAGCT
TCTACTCTTTTTCCCTTTTCTTGGGTTTGTTTTGATGCCATTATCTCAAG
AATGCATAATAGTAGGATAAAATTCGTTCAGCTCGGCTAGCGAGATATCT
TCAAAGAGTAAAGCGCATCTGCGTGTAAAATTAAATTCAAGAAGTGAAAG
AATAAAAGATAGGATTGTAGATTTGAAGTTTAGTGGTAGCGTCAGCAGAT
AAGCCCATGGCAGGTCGACGGATCCCCGGGTTAATTAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAA
TTAATCTCAGAAGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTGAT
TTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACT
TGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGACTCGAC
GGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCT
AATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAG
ACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCTGAAGAAGATTTG
AACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAA
ATTAATCAATCACACCATGGATGTTGATGAACCGAATCCTATTGTGATATCAGATTCTGAGGCCACTGATGAGGAAATTAGTATCATATACGAACCCGAATTCAATGAAAATTATTTATGGGCAGAGGAAAACGTCCAAGAAGCAAGCAGATCACAGAAAATTGTCACTGAGAGGTTGAGCCTGGATTCCACTGCTGGAGAATCTTGTACTCCTTCCGTAGTAACTGATACACAAGTTACTACGGGGCTTCGGTGGTCTTTGAGGAAGAGGAAGGCTATTCAAAAGATGCCATACAGTTTAGAACGCATAAAGCATAGGCAGTTGCTAGAGGGCTATGATATCTCTAGTTTTGACAGCATTTCGAATCAGTTGACCTTACCGAAGAACGCTTCTACAGTGATTCACTCAAATGATATTTTACTTACAAAGAGAACTGGTAAACCCTTAGATGAGCAGAAAGATGTTACAATCGACTCCATTAAACCCGAAAACAGCTCGGTTCAATCTCA
AAGATATGATAGTGATGAAGAAATACCAAAGAAAAGGCATCGTACTTTTA
AAGACCTCGACCAGGATATAGTATTTCAATCAGGTGATTCTACGGAAGAT
GAACAAGACTTGGCTAGTACAAATTTACAGAATACTCAAAATGACGAAGT
AATATTTAGAGGAAGAGTATTGAATGTAAGAACTGGTTACCGTGGGGTCC
TGCCAAGAGTTGCTTGGGAAAAGTCGTTGCAAAAGCAGCAGTCTTCAAAA
GTCACGAAAAGAAAAACTCAATTATTGAACCATAAGGGTGTGGCTAAGAG
GAAAATGAACAGAAGCGCCCATATTGAAGATGAAGAACAGAACTTATTGA
ACGATCTTATTGCACCTGATGACGAACTGGATATCGAGGAAAACGCCCCT
CCAGATATATACTTAGGAAATCTTCCAGAGGATCGTGAGGCTAACGAAAA
GGAATTAAAGGAATTGCAAGAATATTATGAGAGCAAGTATAGTGAAGACG
CACAATCGGCTGGAACTTCTGGTTTTAATTTAAATGAGGAATACCGCAAT
GAACCAGTGTATGAGCTGGAATATGACGGACCGGGGAGCTGCATATCACA
TGTGTCTTATAAAGACCAGCCTATTATTTATTTGAATTCACGGCATTCAG
ATAGCGGATTTAGTGAGCAATACAATATATCTGCCGAAGATAATCAAAGT
GTAATTTCCCTAGATGCTGCTGAAGAGCATAACGATGGTATAATTGACAA
GATGTTGGTTAAACCTAAAAGAATTAAAGCAACCAATGATGCCAACTTTT
TGAATACAAAAAGTAAGAGAGTGAGAAGATATAAGTACAAATATCGAAAT
TCTTGTCTGGCCCCAAGTACAAAAGCTATCAAAGTCGGAAAACGGTCAGC
GCATAAATCTCATTTGGCAGCTAATAATCCAGTTTCATTTGTTTCTAAGA
AGAATCATGTAATTGATGATTATTTTTTTGAAGAATTAGAATCTCAATCC
CTAGAACAGGATGACAGCTCTTCTCTCAAGCCACAGAAAAAAAGAAGGAA
GAAGAAAGCACCTATATACTCAAGTTTCTCCGCTGATCTTGAATCGAGAC
GAAAACCAGTTTTCAATACTGTTGTAGAGGTTCCCACGAACCGATACGCT
TTCACAAAGCCAAATGTACGTAATAGGGACAGCATTAATCATGACATGGA
ATTTGAGGAAGAAGACTCCAATCAAGAGCTTGGACCAATAATGGTCGTAC
TTGACTCAATTTTACTAAAAAAACCTTTTGAGCCACCTAATTTCTTCAAG
ATCCAGTTGTCAGACAAGAGTTTCTTGCTATCAAAATTGAATCCTGCAGA
TATAGCTACATCATTACAGAAAATATTCAGAGTAATCATTGATAAAGGTA
TCACTGATACTGAACTAGTTCACTTTAACGAAAGCCTTATTGCTTTTTTG
GTGCACCTTGACATGCCTGAACTATTCGACTTAATAGGTGAATTTCATCG
TGAATTTAGATCGAAGGTCAATAGCTTGAGGAAAAAAGCTAAACCAATCC
ATTTTTTTCAAATTGCGGCATGCCAATTAATGTTTCTAGAAATATCAAGA
TATAACAAAATTTCAGCCGCTGCAAAATTCGACATGGATGTCAAACTCTT
AGATCATATTGTGTCTTTCTTCAAATTACTTTCTGTCTGTTATGATTCTG
TTATGAAAAATCCAATGCAATATCTGTACACAAGTTATTATATTCTCTCT
GCTGTCGTTGATGTCATACATAAAAAGGAAGCTTTGTGGGATTTATTTCA
AAAGCACCCCTTTTCGCCTCATATTTCACTGTTGCTGGTCAACATATTTC
CTACGAAAGTCTGCCGCTGGCAGGTTTTGAGATTAGATTCTGAATTCCAA
CCCTTGAGTTCGGCATTTCGGTTCATCAATTATTGCATTGAGACTTGTAA
TTGGAATGTTACAAATTCTTTGATTCTTTCTCTCGATCGCATATTCAAAC
GGAGAAGATTTTCGGACTTTGAAGAAGAGTCTGATTTGTCACAAAACAAT
AAGATAATTTATCCTCCAACGAATCAGCTTACGAGTCGATTAATGTTTAA
CAGATATTTGCATCTACTGACGTTGTGTGAATTGTCTAGTTCTGATACAC
AACGTGTGATACCGATGGGTGATATTTCTATGAATGATTCTCTATCTGTT
TTGAAGAACCGCCTCAACCTACTAATTGTTCTAGCTACACGTTTTGATTT
AAACTTAGAGAAGCGCTTCCAAGAGCTAACACGACCGTTATATAGTAAGG
AATATTTAAACTTGCATACACAGAACACAGTCAGGACGATAACAACATTG
ATCATGCAGGCCAGCCTTTCATTCCTGGAAATCAGTCGAATCAAAAATCA
TCCATTCAGTGGAAAATTTATTGCGTCATTATTTGATAAACTAGTACTTC
AACAACCTTCCATATCGGGAGTAACAGAAAATTTTTTGAAAGAATTCACA
AATTTAGTCTCCAAAATGAAAAGAAAAAGCGTGAGTATGCTCAAATTTCT
GTATCCTTCACTGGTGGCCATGTCTCAAGAAAATATATTCGAGTCGTCTT
TTTTTTTATTATTACAGGTATATTTGAAGAGCTTAGATGTGCTGGGTCCA
ACTTGGGTGCAAAATTATCTTTTTCAGTTTATAAAGAGCAAGGCTCAAGA
AAATGAAAGATGGATAGAATGCTATTGTCAGATCGGAAAATTCCTCGTAG
ATTCTGGTATATTTACCTGGTGGACTTTCTTTACCTATAACGGCTTAGAT
GCTGCATTACACTTTCAGCTTGCATTTCACTCCCTTATTATCGATTTCTG
TGATACAGACTCTTTTGAATTACTGAAAAAACCGCTTTATTCCATTGCTT
CTGATTTGCTGCTCATTTCCAAAGATGACGCTTTCTATCACTTTCTTTCC
AATCTGTTGAAGAGGGCACATATCATAGTAGCAGATTTGAAACCTGTTTC
CGATGAGAATGAACTCCTGCGATTGGCTTATATTTTTTCTAAAGCATTGA
AAAAAAATGCATATCAAGATCTATTAGCTGTTTTCTTATCCCTGGCGAAA
AAACATTACGATGAAGGGGACATCAGTAGAAATTTCTTGGCAAAATATCT
CGAATTTCTTAACAAAAATTGCTTGACTGAGCTAAGAAATAATCAACTCT
TCATCAGTCTAAGAAGGGAACTTGGTATTTCAAGTGATGAAGACGAAAAA
TGTGCGTTCTGGGACTCATTCAATGAAGCAGGTGATATACTTTCCAAAGC
TGCATTTGTTGAAACCGGTATTGTTCAAGCCTGTTGCACTGGGAATGAAA
TTGATGGATATCTTGACAATTTAAGCACTTTATTCACCTCCACCATGTTA
GAGAGCCCTTTTGCATTTTTTTCTGACCTGGTGATAGCACACATATTTGA
GAACAGACCTTTTTTCGACGTGAATATCAAGAATTTTCTTCTATCTCACT
TCATAGACCTTTTCAACAAGGTTTTAAAAATGAAATTCGAGCAGGTATCT
CCTGATGAATTTGCTGAACTTTGCAAAGTATACAGGGCGTTATGTATTGA
GTGCGCAACAGACGACACCTTTAATAGTAATAGTGATTTGATCGCCGCAA
AAGATGCGTTTTTGGTATCTGTGTTGAGAATCGCAGATGGGTTTTGGGAG
CATGACAAACTTTTGCAGCTAAGGATGCTTGATAGTAATATGAACATACC
TAATCAGATTCCTCATACTACTTTGCAAAGTTCGCTATCTGCGATTGTAA
TAAAGATAATAGAAAGTAATATAGGAAAAATTGAGGCGTCTGAACCTTTC
AAAACCTTCAAGAATACGTAAGCGGCCGC

Amino Acid Sequence

MAGRRIPGLINGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLN
GEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDL
NGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEED
LNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLINHTMDVDEPNPIVIS
DSEATDEEISIIYEPEFNENYLWAEENVQEASRSQKIVTERLSLDSTAGE
SCTPSVVTDTQVTTGLRWSLRKRKAIQKMPYSLERIKHRQLLEGYDISSF
DSISNQLTLPKNASTVIHSNDILLTKRTGKPLDEQKDVTIDSIKPENSSV
QSQRYDSDEEIPKKRHRTFKDLDQDIVFQSGDSTEDEQDLASTNLQNTQN
DEVIFRGRVLNVRTGYRGVLPRVAWEKSLQKQQSSKVTKRKTQLLNHKGV
AKRKMNRSAHIEDEEQNLLNDLIAPDDELDIEENAPPDIYLGNLPEDREA
NEKELKELQEYYESKYSEDAQSAGTSGFNLNEEYRNEPVYELEYDGPGSC
ISHVSYKDQPIIYLNSRHSDSGFSEQYNISAEDNQSVISLDAAEEHNDGI
IDKMLVKPKRIKATNDANFLNTKSKRVRRYKYKYRNSCLAPSTKAIKVGK
RSAHKSHLAANNPVSFVSKKNHVIDDYFFEELESQSLEQDDSSSLKPQKK
RRKKKAPIYSSFSADLESRRKPVFNTVVEVPTNRYAFTKPNVRNRDSINH
DMEFEEEDSNQELGPIMVVLDSILLKKPFEPPNFFKIQLSDKSFLLSKLN
PADIATSLQKIFRVIIDKGITDTELVHFNESLIAFLVHLDMPELFDLIGE
FHREFRSKVNSLRKKAKPIHFFQIAACQLMFLEISRYNKISAAAKFDMDV
KLLDHIVSFFKLLSVCYDSVMKNPMQYLYTSYYILSAVVDVIHKKEALWD
LFQKHPFSPHISLLLVNIFPTKVCRWQVLRLDSEFQPLSSAFRFINYCIE
TCNWNVTNSLILSLDRIFKRRRFSDFEEESDLSQNNKIIYPPTNQLTSRL
MFNRYLHLLTLCELSSSDTQRVIPMGDISMNDSLSVLKNRLNLLIVLATR
FDLNLEKRFQELTRPLYSKEYLNLHTQNTVRTITTLIMQASLSFLEISRI
KNHPFSGKFIASLFDKLVLQQPSISGVTENFLKEFTNLVSKMKRKSVSML
KFLYPSLVAMSQENIFESSFFLLLQVYLKSLDVLGPTWVQNYLFQFIKSK
AQENERWIECYCQIGKFLVDSGIFTWWTFFTYNGLDAALHFQLAFHSLII
DFCDTDSFELLKKPLYSIASDLLLISKDDAFYHFLSNLLKRAHIIVADLK
PVSDENELLRLAYIFSKALKKNAYQDLLAVFLSLAKKHYDEGDISRNFLA
KYLEFLNKNCLTELRNNQLFISLRRELGISSDEDEKCAFWDSFNEAGDIL
SKAAFVETGIVQACCTGNEIDGYLDNLSTLFTSTMLESPFAFFSDLVIAH
IFENRPFFDVNIKNFLLSHFIDLFNKVLKMKFEQVSPDEFAELCKVYRAL
CIECATDDTFNSNSDLIAAKDAFLVSVLRIADGFWEHDKLLQLRMLDSNM
NIPNQIPHTTLQSSLSAIVIKIIESNIGKIEASEPFKTFKNT*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11462
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid for 13Myc-tagged Mms22.