cDNA Clone DPB2 (yeast)

• Plasmid: pRS415 Pro-DPB2-6His-3Myc
• Parent Plasmid: pRS415
• Genbank: AAB68109
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCCTCAATATTTTTCATGTCTCGACGAGACTCCAGGTATCTTTGAT
CGATGTTTGCGTCGTCCAACACCGACTGCAACAGATGACACTTTTCCTTG
ACAAATTGTAGTTCCTTTTGTGTAGAGTGCAGCTGCTGCCGCAATTGCGC
AAACTTTGCTTGCCATTGTAGAGGCACACAATCCTGAGAATTTTTAGACA
ATGGGACAACGCTTTCTTCTTCCTCATCTGCGGGAGACCCCCATACAAAC
GCTTGGATCTTGGCAAGAGTCTTAGCAATATTGAATCCAAACGCGTGTTG
TTGAGCCCTCTTTTTGGAGATATTCTTGTAGTTTTTAGAACGTGTCCTTT
GTGAAGCGTGTCTAACATGATTTCTTTCGCGAGGCACAACCACCAATTTG
CGCTCTTTTCGTATCCCCAATGTCTTTTCCTGAATTCCCATTGTGATCTT
CAATGCCAACCCAACCTTCTTTTTACTTTGTTGGGTCTAAATTATGTTAC
TTTGTCCATTTCATCTCTTTTCAAGATGGAATCTAAAAGAAGAAAAACGA
CAAAAGGAACAAAAGTGATGTGACGCGTTATTTTTCGCGTGACATAATAG
AAAAATATTTCAACTGGGCATCTTTAACACATGTTACAATAAAGTTGAGA
TGACTTGTTTATGCCTTTAAAGTTTGTAAGTGGAACTAGAAGCGTCTATG
TGTGAAATGTTTGGCTCTGGGAATGTTCTGCCTGTTAAAATTCAGCCTCC
GCTGCTTAGGCCACTGGCGTATAGAGTTTTATCAAGAAAATATGGTTTAT
CAATTAAATCTGATGGACTGTCTGCTCTTGCAGAATTCGTTGGCACTAAT
ATAGGTGCTAACTGGAGACAAGGGCCTGCTACAATAAAATTTCTCGAGCA
ATTTGCTGCAGTATGGAAACAACAAGAAAGAGGCCTTTTCATCGACCAGA
GCGGGGTTAAAGAAGTTATACAGGAGATGAAAGAACGTGAGAAAGTCGAA
TGGAGTCATGAACACCCTATTCAACATGAGGAGAATATCTTGGGACGGAC
AGATGACGATGAGAACAACAGTGACGATGAAATGCCGATCGCTGCTGATT
CATCCTTACAAAATGTTTCATTATCTTCACCCATGCGACAGCCCACCGAA
AGAGATGAATATAAACAGCCTTTCAAGCCAGAAAGCTCCAAGGCGTTGGA
CTGGAGGGACTACTTCAAAGTTATCAATGCTTCTCAGCAACAAAGATTCT
CATACAATCCGCACAAAATGCAATTTATTTTCGTCCCCAATAAAAAGCAG
AATGGGCTAGGAGGCATTGCGGGTTTTCTACCAGATATAGAGGACAAAGT
TCAAATGTTCTTGACAAGATATTACCTCACGAACGACAGAGTCATGAGAA
ATGAAAACTTCCAAAACAGTGACATGTTTAATCCATTATCATCTATGGTG
TCTTTACAAAATGAACTGTCCAATACTAACCGACAGCAGCAATCCAGCAG
TATGAGTATCACCCCAATAAAAAACCTACTAGGTAGGGATGCTCAAAACT
TTCTACTATTGGGGCTCTTAAATAAGAACTTTAAGGGCAATTGGTCACTG
GAAGATCCGTCTGGATCTGTCGAAATTGACATTTCCCAAACTATCCCTAC
ACAAGGCCATTACTACGTGCCAGGTTGCATGGTTCTTGTGGAAGGAATAT
ACTATTCTGTAGGAAATAAATTCCATGTTACCTCCATGACTTTACCCCCT
GGTGAGAGAAGAGAAATTACATTAGAAACGATAGGTAATCTCGATCTCTT
AGGAATACATGGCATTTCTAATAACAATTTCATTGCTCGTTTGGATAAAG
ATTTGAAGATTAGATTACACCTTTTGGAGAAAGAATTAACAGATCATAAA
TTTGTAATTCTCGGCGCAAATTTGTTCCTAGATGATTTGAAAATTATGAC
TGCACTCAGCAAAATTTTGCAAAAATTAAATGATGACCCACCGACCCTAT
TAATTTGGCAAGGTTCTTTCACTTCAGTTCCCGTTTTCGCATCAATGAGT
AGCCGGAATATAAGTAGTTCCACTCAATTTAAGAATAATTTCGATGCCTT
GGCGACGCTTCTGTCAAGATTTGACAATTTGACCGAAAATACCACAATGA
TATTTATTCCAGGTCCTAACGATTTATGGGGGTCGATGGTGTCATTGGGG
GCAAGTGGGACATTACCGCAAGATCCAATTCCTAGTGCGTTTACCAAAAA
AATCAACAAGGTCTGTAAAAACGTTGTATGGAGCTCAAATCCAACTAGAA
TAGCATACTTATCCCAAGAAATAGTCATTTTCAGGGACGATTTATCCGGA
AGATTCAAAAGACACCGTTTGGAATTCCCATTCAACGAGAGCGAAGATGT
TTATACTGAAAACGATAACATGATGTCTAAAGACACCGATATTGTACCAA
TCGATGAATTAGTTAAAGAACCAGACCAGTTACCACAAAAGGTTCAAGAA
ACAAGAAAACTCGTTAAAACAATACTAGACCAGGGTCATTTATCGCCATT
TCTTGATTCCTTGCGCCCAATTTCATGGGATTTGGACCACACTTTGACAC
TTTGCCCAATACCATCGACAATGGTTCTTTGCGACACTACTTCTGCACAA
TTTGATTTGACATACAACGGTTGTAAAGTAATTAACCCTGGAAGTTTCAT
TCATAATAGACGTGCTAGGTATATGGAGTACGTTCCATCCTCAAAGAAAA
CTATACAAGAAGAGATATATATCCACCATCACCATCACCACGGTGAACAA
AAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGA
AGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCTGAAGAAG
ATTTGGGTTAAGCGGCCGC

Amino Acid Sequence

MFGSGNVLPVKIQPPLLRPLAYRVLSRKYGLSIKSDGLSALAEFVGTNIG
ANWRQGPATIKFLEQFAAVWKQQERGLFIDQSGVKEVIQEMKEREKVEWS
HEHPIQHEENILGRTDDDENNSDDEMPIAADSSLQNVSLSSPMRQPTERD
EYKQPFKPESSKALDWRDYFKVINASQQQRFSYNPHKMQFIFVPNKKQNG
LGGIAGFLPDIEDKVQMFLTRYYLTNDRVMRNENFQNSDMFNPLSSMVSL
QNELSNTNRQQQSSSMSITPIKNLLGRDAQNFLLLGLLNKNFKGNWSLED
PSGSVEIDISQTIPTQGHYYVPGCMVLVEGIYYSVGNKFHVTSMTLPPGE
RREITLETIGNLDLLGIHGISNNNFIARLDKDLKIRLHLLEKELTDHKFV
ILGANLFLDDLKIMTALSKILQKLNDDPPTLLIWQGSFTSVPVFASMSSR
NISSSTQFKNNFDALATLLSRFDNLTENTTMIFIPGPNDLWGSMVSLGAS
GTLPQDPIPSAFTKKINKVCKNVVWSSNPTRIAYLSQEIVIFRDDLSGRF
KRHRLEFPFNESEDVYTENDNMMSKDTDIVPIDELVKEPDQLPQKVQETR
KLVKTILDQGHLSPFLDSLRPISWDLDHTLTLCPIPSTMVLCDTTSAQFD
LTYNGCKVINPGSFIHNRRARYMEYVPSSKKTIQEEIYIHHHHHHGEQKL
ISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLG*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11449
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee