cDNA Clone Mms22 (yeast)

• Plasmid: pRS415 Pro-Mms22 (ATG=Nco1)
• Parent Plasmid: pRS415
• Genbank: PCR from pRS416 Pro-Mms22 (ATG=Nco1) DU11356
• Species: Yeast

Sequence of Insert

GGATCCATTGCTTGTTTAACCTTTAGTTTGTCTAAAAGTGTCACTATAAT
CTCTCTGATTTTTGGCAGATGTTTATTTAAAGTTTCATCAGATGGTGGTT
CCCTCAATGTCACTTCCAATACACGTCTTAAAGCCATTGGCACATCACCA
ACATCTGACATGTCAACTTTCGCATGCATGAAGAATTTCGATACGACTTT
GAAGTCATTGCCCAACTGAACGTATGCATCAGATACTAATCTTCCAGAGG
TTGTCCCCTTAGACCATTGTGTTAAGACCTGGAGGAGGTGCTTGGTGGAC
ATCAGCAACTTTGTTACTGTCGTTTCAATACTACTACTGCTAGAAGCAGT
TCTTTTAATCTTTGGGGTGCCATACGTAGGGTCATCCACGGCCATCTTCA
TCTAATTTTAAAATAATACGAGGATTACTACACCAATTTGGCAGCCAGCT
TCTACTCTTTTTCCCTTTTCTTGGGTTTGTTTTGATGCCATTATCTCAAG
AATGCATAATAGTAGGATAAAATTCGTTCAGCTCGGCTAGCGAGATATCT
TCAAAGAGTAAAGCGCATCTGCGTGTAAAATTAAATTCAAGAAGTGAAAG
AATAAAAGATAGGATTGTAGATTTGAAGTTTAGTGGTAGCGTCAGCAGAT
AAGCCCATGGATGTTGATGAACCGAATCCTATTGTGATATCAGATTCTGA
GGCCACTGATGAGGAAATTAGTATCATATACGAACCCGAATTCAATGAAA
ATTATTTATGGGCAGAGGAAAACGTCCAAGAAGCAAGCAGATCACAGAAA
ATTGTCACTGAGAGGTTGAGCCTGGATTCCACTGCTGGAGAATCTTGTAC
TCCTTCCGTAGTAACTGATACACAAGTTACTACGGGGCTTCGGTGGTCTT
TGAGGAAGAGGAAGGCTATTCAAAAGATGCCATACAGTTTAGAACGCATA
AAGCATAGGCAGTTGCTAGAGGGCTATGATATCTCTAGTTTTGACAGCAT
TTCGAATCAGTTGACCTTACCGAAGAACGCTTCTACAGTGATTCACTCAA
ATGATATTTTACTTACAAAGAGAACTGGTAAACCCTTAGATGAGCAGAAA
GATGTTACAATCGACTCCATTAAACCCGAAAACAGCTCGGTTCAATCTCA
AAGATATGATAGTGATGAAGAAATACCAAAGAAAAGGCATCGTACTTTTA
AAGACCTCGACCAGGATATAGTATTTCAATCAGGTGATTCTACGGAAGAT
GAACAAGACTTGGCTAGTACAAATTTACAGAATACTCAAAATGACGAAGT
AATATTTAGAGGAAGAGTATTGAATGTAAGAACTGGTTACCGTGGGGTCC
TGCCAAGAGTTGCTTGGGAAAAGTCGTTGCAAAAGCAGCAGTCTTCAAAA
GTCACGAAAAGAAAAACTCAATTATTGAACCATAAGGGTGTGGCTAAGAG
GAAAATGAACAGAAGCGCCCATATTGAAGATGAAGAACAGAACTTATTGA
ACGATCTTATTGCACCTGATGACGAACTGGATATCGAGGAAAACGCCCCT
CCAGATATATACTTAGGAAATCTTCCAGAGGATCGTGAGGCTAACGAAAA
GGAATTAAAGGAATTGCAAGAATATTATGAGAGCAAGTATAGTGAAGACG
CACAATCGGCTGGAACTTCTGGTTTTAATTTAAATGAGGAATACCGCAAT
GAACCAGTGTATGAGCTGGAATATGACGGACCGGGGAGCTGCATATCACA
TGTGTCTTATAAAGACCAGCCTATTATTTATTTGAATTCACGGCATTCAG
ATAGCGGATTTAGTGAGCAATACAATATATCTGCCGAAGATAATCAAAGT
GTAATTTCCCTAGATGCTGCTGAAGAGCATAACGATGGTATAATTGACAA
GATGTTGGTTAAACCTAAAAGAATTAAAGCAACCAATGATGCCAACTTTT
TGAATACAAAAAGTAAGAGAGTGAGAAGATATAAGTACAAATATCGAAAT
TCTTGTCTGGCCCCAAGTACAAAAGCTATCAAAGTCGGAAAACGGTCAGC
GCATAAATCTCATTTGGCAGCTAATAATCCAGTTTCATTTGTTTCTAAGA
AGAATCATGTAATTGATGATTATTTTTTTGAAGAATTAGAATCTCAATCC
CTAGAACAGGATGACAGCTCTTCTCTCAAGCCACAGAAAAAAAGAAGGAA
GAAGAAAGCACCTATATACTCAAGTTTCTCCGCTGATCTTGAATCGAGAC
GAAAACCAGTTTTCAATACTGTTGTAGAGGTTCCCACGAACCGATACGCT
TTCACAAAGCCAAATGTACGTAATAGGGACAGCATTAATCATGACATGGA
ATTTGAGGAAGAAGACTCCAATCAAGAGCTTGGACCAATAATGGTCGTAC
TTGACTCAATTTTACTAAAAAAACCTTTTGAGCCACCTAATTTCTTCAAG
ATCCAGTTGTCAGACAAGAGTTTCTTGCTATCAAAATTGAATCCTGCAGA
TATAGCTACATCATTACAGAAAATATTCAGAGTAATCATTGATAAAGGTA
TCACTGATACTGAACTAGTTCACTTTAACGAAAGCCTTATTGCTTTTTTG
GTGCACCTTGACATGCCTGAACTATTCGACTTAATAGGTGAATTTCATCG
TGAATTTAGATCGAAGGTCAATAGCTTGAGGAAAAAAGCTAAACCAATCC
ATTTTTTTCAAATTGCGGCATGCCAATTAATGTTTCTAGAAATATCAAGA
TATAACAAAATTTCAGCCGCTGCAAAATTCGACATGGATGTCAAACTCTT
AGATCATATTGTGTCTTTCTTCAAATTACTTTCTGTCTGTTATGATTCTG
TTATGAAAAATCCAATGCAATATCTGTACACAAGTTATTATATTCTCTCT
GCTGTCGTTGATGTCATACATAAAAAGGAAGCTTTGTGGGATTTATTTCA
AAAGCACCCCTTTTCGCCTCATATTTCACTGTTGCTGGTCAACATATTTC
CTACGAAAGTCTGCCGCTGGCAGGTTTTGAGATTAGATTCTGAATTCCAA
CCCTTGAGTTCGGCATTTCGGTTCATCAATTATTGCATTGAGACTTGTAA
TTGGAATGTTACAAATTCTTTGATTCTTTCTCTCGATCGCATATTCAAAC
GGAGAAGATTTTCGGACTTTGAAGAAGAGTCTGATTTGTCACAAAACAAT
AAGATAATTTATCCTCCAACGAATCAGCTTACGAGTCGATTAATGTTTAA
CAGATATTTGCATCTACTGACGTTGTGTGAATTGTCTAGTTCTGATACAC
AACGTGTGATACCGATGGGTGATATTTCTATGAATGATTCTCTATCTGTT
TTGAAGAACCGCCTCAACCTACTAATTGTTCTAGCTACACGTTTTGATTT
AAACTTAGAGAAGCGCTTCCAAGAGCTAACACGACCGTTATATAGTAAGG
AATATTTAAACTTGCATACACAGAACACAGTCAGGACGATAACAACATTG
ATCATGCAGGCCAGCCTTTCATTCCTGGAAATCAGTCGAATCAAAAATCA
TCCATTCAGTGGAAAATTTATTGCGTCATTATTTGATAAACTAGTACTTC
AACAACCTTCCATATCGGGAGTAACAGAAAATTTTTTGAAAGAATTCACA
AATTTAGTCTCCAAAATGAAAAGAAAAAGCGTGAGTATGCTCAAATTTCT
GTATCCTTCACTGGTGGCCATGTCTCAAGAAAATATATTCGAGTCGTCTT
TTTTTTTATTATTACAGGTATATTTGAAGAGCTTAGATGTGCTGGGTCCA
ACTTGGGTGCAAAATTATCTTTTTCAGTTTATAAAGAGCAAGGCTCAAGA
AAATGAAAGATGGATAGAATGCTATTGTCAGATCGGAAAATTCCTCGTAG
ATTCTGGTATATTTACCTGGTGGACTTTCTTTACCTATAACGGCTTAGAT
GCTGCATTACACTTTCAGCTTGCATTTCACTCCCTTATTATCGATTTCTG
TGATACAGACTCTTTTGAATTACTGAAAAAACCGCTTTATTCCATTGCTT
CTGATTTGCTGCTCATTTCCAAAGATGACGCTTTCTATCACTTTCTTTCC
AATCTGTTGAAGAGGGCACATATCATAGTAGCAGATTTGAAACCTGTTTC
CGATGAGAATGAACTCCTGCGATTGGCTTATATTTTTTCTAAAGCATTGA
AAAAAAATGCATATCAAGATCTATTAGCTGTTTTCTTATCCCTGGCGAAA
AAACATTACGATGAAGGGGACATCAGTAGAAATTTCTTGGCAAAATATCT
CGAATTTCTTAACAAAAATTGCTTGACTGAGCTAAGAAATAATCAACTCT
TCATCAGTCTAAGAAGGGAACTTGGTATTTCAAGTGATGAAGACGAAAAA
TGTGCGTTCTGGGACTCATTCAATGAAGCAGGTGATATACTTTCCAAAGC
TGCATTTGTTGAAACCGGTATTGTTCAAGCCTGTTGCACTGGGAATGAAA
TTGATGGATATCTTGACAATTTAAGCACTTTATTCACCTCCACCATGTTA
GAGAGCCCTTTTGCATTTTTTTCTGACCTGGTGATAGCACACATATTTGA
GAACAGACCTTTTTTCGACGTGAATATCAAGAATTTTCTTCTATCTCACT
TCATAGACCTTTTCAACAAGGTTTTAAAAATGAAATTCGAGCAGGTATCT
CCTGATGAATTTGCTGAACTTTGCAAAGTATACAGGGCGTTATGTATTGA
GTGCGCAACAGACGACACCTTTAATAGTAATAGTGATTTGATCGCCGCAA
AAGATGCGTTTTTGGTATCTGTGTTGAGAATCGCAGATGGGTTTTGGGAG
CATGACAAACTTTTGCAGCTAAGGATGCTTGATAGTAATATGAACATACC
TAATCAGATTCCTCATACTACTTTGCAAAGTTCGCTATCTGCGATTGTAA
TAAAGATAATAGAAAGTAATATAGGAAAAATTGAGGCGTCTGAACCTTTC
AAAACCTTCAAGAATACGTAAGCGGCCGC

Amino Acid Sequence

MDVDEPNPIVISDSEATDEEISIIYEPEFNENYLWAEENVQEASRSQKIV
TERLSLDSTAGESCTPSVVTDTQVTTGLRWSLRKRKAIQKMPYSLERIKH
RQLLEGYDISSFDSISNQLTLPKNASTVIHSNDILLTKRTGKPLDEQKDV
TIDSIKPENSSVQSQRYDSDEEIPKKRHRTFKDLDQDIVFQSGDSTEDEQ
DLASTNLQNTQNDEVIFRGRVLNVRTGYRGVLPRVAWEKSLQKQQSSKVT
KRKTQLLNHKGVAKRKMNRSAHIEDEEQNLLNDLIAPDDELDIEENAPPD
IYLGNLPEDREANEKELKELQEYYESKYSEDAQSAGTSGFNLNEEYRNEP
VYELEYDGPGSCISHVSYKDQPIIYLNSRHSDSGFSEQYNISAEDNQSVI
SLDAAEEHNDGIIDKMLVKPKRIKATNDANFLNTKSKRVRRYKYKYRNSC
LAPSTKAIKVGKRSAHKSHLAANNPVSFVSKKNHVIDDYFFEELESQSLE
QDDSSSLKPQKKRRKKKAPIYSSFSADLESRRKPVFNTVVEVPTNRYAFT
KPNVRNRDSINHDMEFEEEDSNQELGPIMVVLDSILLKKPFEPPNFFKIQ
LSDKSFLLSKLNPADIATSLQKIFRVIIDKGITDTELVHFNESLIAFLVH
LDMPELFDLIGEFHREFRSKVNSLRKKAKPIHFFQIAACQLMFLEISRYN
KISAAAKFDMDVKLLDHIVSFFKLLSVCYDSVMKNPMQYLYTSYYILSAV
VDVIHKKEALWDLFQKHPFSPHISLLLVNIFPTKVCRWQVLRLDSEFQPL
SSAFRFINYCIETCNWNVTNSLILSLDRIFKRRRFSDFEEESDLSQNNKI
IYPPTNQLTSRLMFNRYLHLLTLCELSSSDTQRVIPMGDISMNDSLSVLK
NRLNLLIVLATRFDLNLEKRFQELTRPLYSKEYLNLHTQNTVRTITTLIM
QASLSFLEISRIKNHPFSGKFIASLFDKLVLQQPSISGVTENFLKEFTNL
VSKMKRKSVSMLKFLYPSLVAMSQENIFESSFFLLLQVYLKSLDVLGPTW
VQNYLFQFIKSKAQENERWIECYCQIGKFLVDSGIFTWWTFFTYNGLDAA
LHFQLAFHSLIIDFCDTDSFELLKKPLYSIASDLLLISKDDAFYHFLSNL
LKRAHIIVADLKPVSDENELLRLAYIFSKALKKNAYQDLLAVFLSLAKKH
YDEGDISRNFLAKYLEFLNKNCLTELRNNQLFISLRRELGISSDEDEKCA
FWDSFNEAGDILSKAAFVETGIVQACCTGNEIDGYLDNLSTLFTSTMLES
PFAFFSDLVIAHIFENRPFFDVNIKNFLLSHFIDLFNKVLKMKFEQVSPD
EFAELCKVYRALCIECATDDTFNSNSDLIAAKDAFLVSVLRIADGFWEHD
KLLQLRMLDSNMNIPNQIPHTTLQSSLSAIVIKIIESNIGKIEASEPFKT
FKNT*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11446
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid for untagged Mms22.