cDNA Clone DPB4 (yeast)

• Plasmid: pRS415 DPB4E ATG=Nco1
• Parent Plasmid: pRS415
• Species: Yeast

Sequence of Insert

GGATCCTTACCAACGGCTTCTTTTGGTGACCCGTTTCCCATTTTTTGACGCTTGGAAGAATCATCGTTAGTTTCCGCGCACCTTTTCTTCTTACTTTTTTTATTATTTCTCTTTTGGCCACATTCCTCACCATATAGGTTGTCCCACGCCTTGATGCATGTAACACTTAGATCTCTATTAAATTGTTGGATGGGATTATAGAAAACAGTTTCCTTTTTAGGGAAAAGAATTTCTGCTTTTCCTTCCTTGACTATATTGAAATCCTCAATATTGATTCTTGGTGCACCATACGCGGTAAAATTCGCT
TTAATTTTGGATATAGCAGCCTTCAACATTCCGTGTAAATTTGCCCGCTT
GAGGGGTATCCTGAAGAAACCTTCCATATCTACATTGTAAGAGGAAAGGT
ATCAACTTATGACTGCTCAGGATCTGTAACTTTGCGATGAGCATTGAATG
CGATGAAAAAAAAATAATGCTTTATCCCTTTGGACGAATAAAGAAAGGCT
CAAATTGGAATAACGTTAAAAACCAGATTCGATCATCAGAGCTTAAAATT
GTTATTCATAGCGGCACAATAGCAGAAGCCACAGAATATTACCTAGAGGT
TTTAGGTGAAAGGAAGGATTTTTTTAATTATTTAATACTTTGAAATAGAA
GTCAAGCCTTTCATTGCTTATTTATATCAGACCATATATTTTTACACACC
ATGGCACCAAAAGGTTGGAGAAAAGACGCCCAAGGGAATTACCCCACTAC
TTCGTATATTAAAGAACAAGAGAACATTACGATTCAGGACCTGTTATTTC
CTAAAAGCACTATTGTGAATTTGGCACGCGAAGTGCCACAGCAAAGCGGC
AAAAAGCTGCTGATAAATAAAGACGCATCACTAGCGTTACAGCGTGGTGC
TACAGTATTTGTTAACCACTTGCTCCTGTTTGCAAGGGAAATTGCAAAGA
GTCAAGATAAAAAAAGCTGCAGTGTAGATGATGTTCTGAGTGCTTTGGAC
CATATAGGACACTCGGCATTGAAGGGTCCGGTTAGAGATAAACTAGATGA
ATACCAAGCTGCTGTTGAACAGAGGAAGAAGGAAAAACTAGATAGTGGTG
AAGTAGATGCTGATGGCGATATAGATATGGGTGAAGATAAGGAGAATGTG
CCCGTGGAAAAGGTTAAAGAGCATGACGAAATAGAAGAACAAGGCGATGC
ATTACAGGACGTAGAAGAGTCCTCCGAAAAGAAACAGAAAACGGAGAGCC
AAGATGTAGAAACTAGAGTTCAAAACCTTGAGCAAACGTAACCCCGCTAT
GGAAACTGTCTAGTAGTGCTTGCCACCACTCCCACAGGATCGTAGTATGT
AACTTGCCTTTATTACCTACATACATAATAAGCTATACTCAGCGGCCGC

Amino Acid Sequence

MAPKGWRKDAQGNYPTTSYIKEQENITIQDLLFPKSTIVNLAREVPQQSG
KKLLINKDASLALQRGATVFVNHLLLFAREIAKSQDKKSCSVDDVLSALD
HIGHSALKGPVRDKLDEYQAAVEQRKKEKLDSGEVDADGDIDMGEDKENV
PVEKVKEHDEIEEQGDALQDVEESSEKKQKTESQDVETRVQNLEQT*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11411
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid for untagged DPB4.; P2A due to Nco1 site.