cDNA Clone Mms22 (yeast)

• Plasmid: pFastBacHTb Mms22
• Parent Plasmid: pFastBac HTb
• Genbank: YSCL8543
• Species: Yeast

Sequence of Insert

GGATCCATGGATGTTGATGAACCGAATCCTATTGTGATATCAGATTCTGAGGCCACTGATGAGGAAATTAGTATCATATACGAACCCGAATTCAATGAAAATTATTTATGGGCAGAGGAAAACGTCCAAGAAGCAAGCAGATCACAGAAAATTGTCACTGAGAGGTTGAGCCTGGATTCCACTGCTGGAGAATCTTGTACTCCTTCCGTAGTAACTGATACACAAGTTACTACGGGGCTTCGGTGGTCTTTGAGGAAGAGGAAGGCTATTCAAAAGATGCCATACAGTTTAGAACGCATAAAGCAT
AGGCAGTTGCTAGAGGGCTATGATATCTCTAGTTTTGACAGCATTTCGAA
TCAGTTGACCTTACCGAAGAACGCTTCTACAGTGATTCACTCAAATGATA
TTTTACTTACAAAGAGAACTGGTAAACCCTTAGATGAGCAGAAAGATGTT
ACAATCGACTCCATTAAACCCGAAAACAGCTCGGTTCAATCTCAAAGATA
TGATAGTGATGAAGAAATACCAAAGAAAAGGCATCGTACTTTTAAAGACC
TCGACCAGGATATAGTATTTCAATCAGGTGATTCTACGGAAGATGAACAA
GACTTGGCTAGTACAAATTTACAGAATACTCAAAATGACGAAGTAATATT
TAGAGGAAGAGTATTGAATGTAAGAACTGGTTACCGTGGGGTCCTGCCAA
GAGTTGCTTGGGAAAAGTCGTTGCAAAAGCAGCAGTCTTCAAAAGTCACG
AAAAGAAAAACTCAATTATTGAACCATAAGGGTGTGGCTAAGAGGAAAAT
GAACAGAAGCGCCCATATTGAAGATGAAGAACAGAACTTATTGAACGATC
TTATTGCACCTGATGACGAACTGGATATCGAGGAAAACGCCCCTCCAGAT
ATATACTTAGGAAATCTTCCAGAGGATCGTGAGGCTAACGAAAAGGAATT
AAAGGAATTGCAAGAATATTATGAGAGCAAGTATAGTGAAGACGCACAAT
CGGCTGGAACTTCTGGTTTTAATTTAAATGAGGAATACCGCAATGAACCA
GTGTATGAGCTGGAATATGACGGACCGGGGAGCTGCATATCACATGTGTC
TTATAAAGACCAGCCTATTATTTATTTGAATTCACGGCATTCAGATAGCG
GATTTAGTGAGCAATACAATATATCTGCCGAAGATAATCAAAGTGTAATT
TCCCTAGATGCTGCTGAAGAGCATAACGATGGTATAATTGACAAGATGTT
GGTTAAACCTAAAAGAATTAAAGCAACCAATGATGCCAACTTTTTGAATA
CAAAAAGTAAGAGAGTGAGAAGATATAAGTACAAATATCGAAATTCTTGT
CTGGCCCCAAGTACAAAAGCTATCAAAGTCGGAAAACGGTCAGCGCATAA
ATCTCATTTGGCAGCTAATAATCCAGTTTCATTTGTTTCTAAGAAGAATC
ATGTAATTGATGATTATTTTTTTGAAGAATTAGAATCTCAATCCCTAGAA
CAGGATGACAGCTCTTCTCTCAAGCCACAGAAAAAAAGAAGGAAGAAGAA
AGCACCTATATACTCAAGTTTCTCCGCTGATCTTGAATCGAGACGAAAAC
CAGTTTTCAATACTGTTGTAGAGGTTCCCACGAACCGATACGCTTTCACA
AAGCCAAATGTACGTAATAGGGACAGCATTAATCATGACATGGAATTTGA
GGAAGAAGACTCCAATCAAGAGCTTGGACCAATAATGGTCGTACTTGACT
CAATTTTACTAAAAAAACCTTTTGAGCCACCTAATTTCTTCAAGATCCAG
TTGTCAGACAAGAGTTTCTTGCTATCAAAATTGAATCCTGCAGATATAGC
TACATCATTACAGAAAATATTCAGAGTAATCATTGATAAAGGTATCACTG
ATACTGAACTAGTTCACTTTAACGAAAGCCTTATTGCTTTTTTGGTGCAC
CTTGACATGCCTGAACTATTCGACTTAATAGGTGAATTTCATCGTGAATT
TAGATCGAAGGTCAATAGCTTGAGGAAAAAAGCTAAACCAATCCATTTTT
TTCAAATTGCGGCATGCCAATTAATGTTTCTAGAAATATCAAGATATAAC
AAAATTTCAGCCGCTGCAAAATTCGACATGGATGTCAAACTCTTAGATCA
TATTGTGTCTTTCTTCAAATTACTTTCTGTCTGTTATGATTCTGTTATGA
AAAATCCAATGCAATATCTGTACACAAGTTATTATATTCTCTCTGCTGTC
GTTGATGTCATACATAAAAAGGAAGCTTTGTGGGATTTATTTCAAAAGCA
CCCCTTTTCGCCTCATATTTCACTGTTGCTGGTCAACATATTTCCTACGA
AAGTCTGCCGCTGGCAGGTTTTGAGATTAGATTCTGAATTCCAACCCTTG
AGTTCGGCATTTCGGTTCATCAATTATTGCATTGAGACTTGTAATTGGAA
TGTTACAAATTCTTTGATTCTTTCTCTCGATCGCATATTCAAACGGAGAA
GATTTTCGGACTTTGAAGAAGAGTCTGATTTGTCACAAAACAATAAGATA
ATTTATCCTCCAACGAATCAGCTTACGAGTCGATTAATGTTTAACAGATA
TTTGCATCTACTGACGTTGTGTGAATTGTCTAGTTCTGATACACAACGTG
TGATACCGATGGGTGATATTTCTATGAATGATTCTCTATCTGTTTTGAAG
AACCGCCTCAACCTACTAATTGTTCTAGCTACACGTTTTGATTTAAACTT
AGAGAAGCGCTTCCAAGAGCTAACACGACCGTTATATAGTAAGGAATATT
TAAACTTGCATACACAGAACACAGTCAGGACGATAACAACATTGATCATG
CAGGCCAGCCTTTCATTCCTGGAAATCAGTCGAATCAAAAATCATCCATT
CAGTGGAAAATTTATTGCGTCATTATTTGATAAACTAGTACTTCAACAAC
CTTCCATATCGGGAGTAACAGAAAATTTTTTGAAAGAATTCACAAATTTA
GTCTCCAAAATGAAAAGAAAAAGCGTGAGTATGCTCAAATTTCTGTATCC
TTCACTGGTGGCCATGTCTCAAGAAAATATATTCGAGTCGTCTTTTTTTT
TATTATTACAGGTATATTTGAAGAGCTTAGATGTGCTGGGTCCAACTTGG
GTGCAAAATTATCTTTTTCAGTTTATAAAGAGCAAGGCTCAAGAAAATGA
AAGATGGATAGAATGCTATTGTCAGATCGGAAAATTCCTCGTAGATTCTG
GTATATTTACCTGGTGGACTTTCTTTACCTATAACGGCTTAGATGCTGCA
TTACACTTTCAGCTTGCATTTCACTCCCTTATTATCGATTTCTGTGATAC
AGACTCTTTTGAATTACTGAAAAAACCGCTTTATTCCATTGCTTCTGATT
TGCTGCTCATTTCCAAAGATGACGCTTTCTATCACTTTCTTTCCAATCTG
TTGAAGAGGGCACATATCATAGTAGCAGATTTGAAACCTGTTTCCGATGA
GAATGAACTCCTGCGATTGGCTTATATTTTTTCTAAAGCATTGAAAAAAA
ATGCATATCAAGATCTATTAGCTGTTTTCTTATCCCTGGCGAAAAAACAT
TACGATGAAGGGGACATCAGTAGAAATTTCTTGGCAAAATATCTCGAATT
TCTTAACAAAAATTGCTTGACTGAGCTAAGAAATAATCAACTCTTCATCA
GTCTAAGAAGGGAACTTGGTATTTCAAGTGATGAAGACGAAAAATGTGCG
TTCTGGGACTCATTCAATGAAGCAGGTGATATACTTTCCAAAGCTGCATT
TGTTGAAACCGGTATTGTTCAAGCCTGTTGCACTGGGAATGAAATTGATG
GATATCTTGACAATTTAAGCACTTTATTCACCTCCACCATGTTAGAGAGC
CCTTTTGCATTTTTTTCTGACCTGGTGATAGCACACATATTTGAGAACAG
ACCTTTTTTCGACGTGAATATCAAGAATTTTCTTCTATCTCACTTCATAG
ACCTTTTCAACAAGGTTTTAAAAATGAAATTCGAGCAGGTATCTCCTGAT
GAATTTGCTGAACTTTGCAAAGTATACAGGGCGTTATGTATTGAGTGCGC
AACAGACGACACCTTTAATAGTAATAGTGATTTGATCGCCGCAAAAGATG
CGTTTTTGGTATCTGTGTTGAGAATCGCAGATGGGTTTTGGGAGCATGAC
AAACTTTTGCAGCTAAGGATGCTTGATAGTAATATGAACATACCTAATCA
GATTCCTCATACTACTTTGCAAAGTTCGCTATCTGCGATTGTAATAAAGA
TAATAGAAAGTAATATAGGAAAAATTGAGGCGTCTGAACCTTTCAAAACC
TTCAAGAATACGTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMDVDEPNPIVISDSEATDEEIS
IIYEPEFNENYLWAEENVQEASRSQKIVTERLSLDSTAGESCTPSVVTDT
QVTTGLRWSLRKRKAIQKMPYSLERIKHRQLLEGYDISSFDSISNQLTLP
KNASTVIHSNDILLTKRTGKPLDEQKDVTIDSIKPENSSVQSQRYDSDEE
IPKKRHRTFKDLDQDIVFQSGDSTEDEQDLASTNLQNTQNDEVIFRGRVL
NVRTGYRGVLPRVAWEKSLQKQQSSKVTKRKTQLLNHKGVAKRKMNRSAH
IEDEEQNLLNDLIAPDDELDIEENAPPDIYLGNLPEDREANEKELKELQE
YYESKYSEDAQSAGTSGFNLNEEYRNEPVYELEYDGPGSCISHVSYKDQP
IIYLNSRHSDSGFSEQYNISAEDNQSVISLDAAEEHNDGIIDKMLVKPKR
IKATNDANFLNTKSKRVRRYKYKYRNSCLAPSTKAIKVGKRSAHKSHLAA
NNPVSFVSKKNHVIDDYFFEELESQSLEQDDSSSLKPQKKRRKKKAPIYS
SFSADLESRRKPVFNTVVEVPTNRYAFTKPNVRNRDSINHDMEFEEEDSN
QELGPIMVVLDSILLKKPFEPPNFFKIQLSDKSFLLSKLNPADIATSLQK
IFRVIIDKGITDTELVHFNESLIAFLVHLDMPELFDLIGEFHREFRSKVN
SLRKKAKPIHFFQIAACQLMFLEISRYNKISAAAKFDMDVKLLDHIVSFF
KLLSVCYDSVMKNPMQYLYTSYYILSAVVDVIHKKEALWDLFQKHPFSPH
ISLLLVNIFPTKVCRWQVLRLDSEFQPLSSAFRFINYCIETCNWNVTNSL
ILSLDRIFKRRRFSDFEEESDLSQNNKIIYPPTNQLTSRLMFNRYLHLLT
LCELSSSDTQRVIPMGDISMNDSLSVLKNRLNLLIVLATRFDLNLEKRFQ
ELTRPLYSKEYLNLHTQNTVRTITTLIMQASLSFLEISRIKNHPFSGKFI
ASLFDKLVLQQPSISGVTENFLKEFTNLVSKMKRKSVSMLKFLYPSLVAM
SQENIFESSFFLLLQVYLKSLDVLGPTWVQNYLFQFIKSKAQENERWIEC
YCQIGKFLVDSGIFTWWTFFTYNGLDAALHFQLAFHSLIIDFCDTDSFEL
LKKPLYSIASDLLLISKDDAFYHFLSNLLKRAHIIVADLKPVSDENELLR
LAYIFSKALKKNAYQDLLAVFLSLAKKHYDEGDISRNFLAKYLEFLNKNC
LTELRNNQLFISLRRELGISSDEDEKCAFWDSFNEAGDILSKAAFVETGI
VQACCTGNEIDGYLDNLSTLFTSTMLESPFAFFSDLVIAHIFENRPFFDV
NIKNFLLSHFIDLFNKVLKMKFEQVSPDEFAELCKVYRALCIECATDDTF
NSNSDLIAAKDAFLVSVLRIADGFWEHDKLLQLRMLDSNMNIPNQIPHTT
LQSSLSAIVIKIIESNIGKIEASEPFKTFKNT*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU11407
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 171050 Daltons