cDNA Clone Mms1 (yeast)

• Plasmid: pRS413 Pro-15HA-Mms1
• Parent Plasmid: pRS413
• Tag: HA
• Species: Yeast

Amino Acid Sequence

MGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAQCGRIFYPYDVPDYAGY
PYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPD
YAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYP
YDVPDYAGSYPYDVPDYAAMVGLRTHGLDRYEHYIRRPSDFGKLELQDWL
NHKSFRVSPNLLIDSSTTREWNEPELFYQNTEDETWVRPCVGPKLEPSMM
MLRYHDSNIGQMPQFCYPISSPINFKPVLKYILQERSELSDGFPQKYNTL
IGSLFDIDKNPETLDDSDIEALDDIEMSSDSGNVKEPKIELQALEEIQQK
HFSLIVSNNGIFQTGSTSITYIQSGISGSIAIKPNNVAILILLTQPSGHL
LSILPLDDGKETYLLQYWNLGQKGQWNIIKHQNEKQFVLIHKELGICKFF
EFHLPFTFQLVNNLTLTDSVIMNGSFFPTNYTDLDPYFIIFITAIRYERI
VYFVIEWNNNEIKKKEVYQLTVFDGEKTNMTIPIGLNACLVETPLKFSLV
SANQIMSGETEFHSFQLKALKGIKSFFPAPLLLLKLQELHPHTFKKFQYC
TIISSSTGNICFCVTERSTIVNGNLKFYELTRFKGLKSISPLPSNPINLD
SRSSSYVLVVISFSRTLELTLSLEDLRCLDKKDVIKPLKNITFKHTIDSS
TEENSQILAFTSSKFYNTHTGSNINDTRNSQVWLTSPNAITQPCIDYKLR
KTHQLIHLKQFQIFRHLRIWKCKNLDIALLQRLGINQSNTESSLIFATDA
VSNNRIFLLDLTMTTTIDNDDPVQGLINIEDLLCDTENETILLNFTKNNL
IQVTRDTIYIDPIGGDKELRKISPGWEFENVTYNDGILIVWNAGLGCVSY
IENIDAVDESGALVSNLSSSKGMSKFFKQLGTVTSVNFQIKESTDDPTKY
DIWILLPDCVIRTPFSDWISDSLDFSDVYILSVQQALINGPYFCSLDYES
YFEVHTLQNNCFKKGSRCTSRVNFQGKDIKFRSFGVNQCLAFSAFEIFVI
NLTPIHDSRELDFYKLKLPHLGNNNSILEVCPDIENNQLFILYSDGLRIL
ELSYLTSNNGNFLLKSTRSKNKKFLYLDKINRMLVLNQDLREWECIRLSD
GKAVGLDSQLLKDDSEEILEIKELPIATEDNPLEKKTVLLISFTSSLKLV
LLTAAKNKISNQIIDSYKLDNSRLLNHLVITPRGEIFFLDYKVMGTDNEM
SFNKLKVTKHCIDQEERNNTTLRLTLETRFTFKSWSTVKTFTVVGDNIIA
TTNMGEKLYLIKDFSSSSDESRRVYPLEMYPDSKVQKIIPLNECCFVVAA
YCGNRNDLDSRLIFYSLPTIKVGLNNETGSLPDEYGNGRVDDIFEVDFPE
GFQFGTMALYDVLHGERHVNRYSEGIRSENDEAEVALRQRRNLLLFWRNH
SSTPKPSLRRAATIVYEDHVSSRYFEDISSILGSTAMRTKRLSPYNAVAL
DKPIQDISYDPAVQTLYVLMADQTIHKFGKDRLPCQDEYEPRWNSGYLVS
RRSIVKSDLICEVGLWNLSDNCKNTV*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11381
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Strategy : Pro-Mms1 cloned into pRS415. ATG mutated to Nco1. 15HA tag cloned in at Nco1 site.; Yeast expression plasmid for 15HA-tagged full length Mms1.; N.B. Mutating ATG start site of Mms1 for insertion of tag results in residue change L2V.