cDNA Clone Mms22 (yeast)

• Plasmid: pGEX6P-2-Mms22-6His
• Parent Plasmid: pGEX6P1
• Genbank: YSCL8543
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCATGGATGTTGATGAACCGAATCCTATTGTGATATCAGATTCTGAGGCCACTGATGAGGAAATTAGTATCATATACGAACCCGAATTCAATGAAAATTATTTATGGGCAGAGGAAAACGTCCAAGAAGCAAGCAGATCACAGAAAATTGTCACTGAGAGGTTGAGCCTGGATTCCACTGCTGGAGAATCTTGTACTCCTTCCGTAGTAACTGATACACAAGTTACTACGGGGCTTCGGTGGTCTTTGAGGAAGAGGAAGGCTATTCAAAAGATGCCATACAGTTTAGAACGCATAAAGCAT
AGGCAGTTGCTAGAGGGCTATGATATCTCTAGTTTTGACAGCATTTCGAA
TCAGTTGACCTTACCGAAGAACGCTTCTACAGTGATTCACTCAAATGATA
TTTTACTTACAAAGAGAACTGGTAAACCCTTAGATGAGCAGAAAGATGTT
ACAATCGACTCCATTAAACCCGAAAACAGCTCGGTTCAATCTCAAAGATA
TGATAGTGATGAAGAAATACCAAAGAAAAGGCATCGTACTTTTAAAGACC
TCGACCAGGATATAGTATTTCAATCAGGTGATTCTACGGAAGATGAACAA
GACTTGGCTAGTACAAATTTACAGAATACTCAAAATGACGAAGTAATATT
TAGAGGAAGAGTATTGAATGTAAGAACTGGTTACCGTGGGGTCCTGCCAA
GAGTTGCTTGGGAAAAGTCGTTGCAAAAGCAGCAGTCTTCAAAAGTCACG
AAAAGAAAAACTCAATTATTGAACCATAAGGGTGTGGCTAAGAGGAAAAT
GAACAGAAGCGCCCATATTGAAGATGAAGAACAGAACTTATTGAACGATC
TTATTGCACCTGATGACGAACTGGATATCGAGGAAAACGCCCCTCCAGAT
ATATACTTAGGAAATCTTCCAGAGGATCGTGAGGCTAACGAAAAGGAATT
AAAGGAATTGCAAGAATATTATGAGAGCAAGTATAGTGAAGACGCACAAT
CGGCTGGAACTTCTGGTTTTAATTTAAATGAGGAATACCGCAATGAACCA
GTGTATGAGCTGGAATATGACGGACCGGGGAGCTGCATATCACATGTGTC
TTATAAAGACCAGCCTATTATTTATTTGAATTCACGGCATTCAGATAGCG
GATTTAGTGAGCAATACAATATATCTGCCGAAGATAATCAAAGTGTAATT
TCCCTAGATGCTGCTGAAGAGCATAACGATGGTATAATTGACAAGATGTT
GGTTAAACCTAAAAGAATTAAAGCAACCAATGATGCCAACTTTTTGAATA
CAAAAAGTAAGAGAGTGAGAAGATATAAGTACAAATATCGAAATTCTTGT
CTGGCCCCAAGTACAAAAGCTATCAAAGTCGGAAAACGGTCAGCGCATAA
ATCTCATTTGGCAGCTAATAATCCAGTTTCATTTGTTTCTAAGAAGAATC
ATGTAATTGATGATTATTTTTTTGAAGAATTAGAATCTCAATCCCTAGAA
CAGGATGACAGCTCTTCTCTCAAGCCACAGAAAAAAAGAAGGAAGAAGAA
AGCACCTATATACTCAAGTTTCTCCGCTGATCTTGAATCGAGACGAAAAC
CAGTTTTCAATACTGTTGTAGAGGTTCCCACGAACCGATACGCTTTCACA
AAGCCAAATGTACGTAATAGGGACAGCATTAATCATGACATGGAATTTGA
GGAAGAAGACTCCAATCAAGAGCTTGGACCAATAATGGTCGTACTTGACT
CAATTTTACTAAAAAAACCTTTTGAGCCACCTAATTTCTTCAAGATCCAG
TTGTCAGACAAGAGTTTCTTGCTATCAAAATTGAATCCTGCAGATATAGC
TACATCATTACAGAAAATATTCAGAGTAATCATTGATAAAGGTATCACTG
ATACTGAACTAGTTCACTTTAACGAAAGCCTTATTGCTTTTTTGGTGCAC
CTTGACATGCCTGAACTATTCGACTTAATAGGTGAATTTCATCGTGAATT
TAGATCGAAGGTCAATAGCTTGAGGAAAAAAGCTAAACCAATCCATTTTT
TTCAAATTGCGGCATGCCAATTAATGTTTCTAGAAATATCAAGATATAAC
AAAATTTCAGCCGCTGCAAAATTCGACATGGATGTCAAACTCTTAGATCA
TATTGTGTCTTTCTTCAAATTACTTTCTGTCTGTTATGATTCTGTTATGA
AAAATCCAATGCAATATCTGTACACAAGTTATTATATTCTCTCTGCTGTC
GTTGATGTCATACATAAAAAGGAAGCTTTGTGGGATTTATTTCAAAAGCA
CCCCTTTTCGCCTCATATTTCACTGTTGCTGGTCAACATATTTCCTACGA
AAGTCTGCCGCTGGCAGGTTTTGAGATTAGATTCTGAATTCCAACCCTTG
AGTTCGGCATTTCGGTTCATCAATTATTGCATTGAGACTTGTAATTGGAA
TGTTACAAATTCTTTGATTCTTTCTCTCGATCGCATATTCAAACGGAGAA
GATTTTCGGACTTTGAAGAAGAGTCTGATTTGTCACAAAACAATAAGATA
ATTTATCCTCCAACGAATCAGCTTACGAGTCGATTAATGTTTAACAGATA
TTTGCATCTACTGACGTTGTGTGAATTGTCTAGTTCTGATACACAACGTG
TGATACCGATGGGTGATATTTCTATGAATGATTCTCTATCTGTTTTGAAG
AACCGCCTCAACCTACTAATTGTTCTAGCTACACGTTTTGATTTAAACTT
AGAGAAGCGCTTCCAAGAGCTAACACGACCGTTATATAGTAAGGAATATT
TAAACTTGCATACACAGAACACAGTCAGGACGATAACAACATTGATCATG
CAGGCCAGCCTTTCATTCCTGGAAATCAGTCGAATCAAAAATCATCCATT
CAGTGGAAAATTTATTGCGTCATTATTTGATAAACTAGTACTTCAACAAC
CTTCCATATCGGGAGTAACAGAAAATTTTTTGAAAGAATTCACAAATTTA
GTCTCCAAAATGAAAAGAAAAAGCGTGAGTATGCTCAAATTTCTGTATCC
TTCACTGGTGGCCATGTCTCAAGAAAATATATTCGAGTCGTCTTTTTTTT
TATTATTACAGGTATATTTGAAGAGCTTAGATGTGCTGGGTCCAACTTGG
GTGCAAAATTATCTTTTTCAGTTTATAAAGAGCAAGGCTCAAGAAAATGA
AAGATGGATAGAATGCTATTGTCAGATCGGAAAATTCCTCGTAGATTCTG
GTATATTTACCTGGTGGACTTTCTTTACCTATAACGGCTTAGATGCTGCA
TTACACTTTCAGCTTGCATTTCACTCCCTTATTATCGATTTCTGTGATAC
AGACTCTTTTGAATTACTGAAAAAACCGCTTTATTCCATTGCTTCTGATT
TGCTGCTCATTTCCAAAGATGACGCTTTCTATCACTTTCTTTCCAATCTG
TTGAAGAGGGCACATATCATAGTAGCAGATTTGAAACCTGTTTCCGATGA
GAATGAACTCCTGCGATTGGCTTATATTTTTTCTAAAGCATTGAAAAAAA
ATGCATATCAAGATCTATTAGCTGTTTTCTTATCCCTGGCGAAAAAACAT
TACGATGAAGGGGACATCAGTAGAAATTTCTTGGCAAAATATCTCGAATT
TCTTAACAAAAATTGCTTGACTGAGCTAAGAAATAATCAACTCTTCATCA
GTCTAAGAAGGGAACTTGGTATTTCAAGTGATGAAGACGAAAAATGTGCG
TTCTGGGACTCATTCAATGAAGCAGGTGATATACTTTCCAAAGCTGCATT
TGTTGAAACCGGTATTGTTCAAGCCTGTTGCACTGGGAATGAAATTGATG
GATATCTTGACAATTTAAGCACTTTATTCACCTCCACCATGTTAGAGAGC
CCTTTTGCATTTTTTTCTGACCTGGTGATAGCACACATATTTGAGAACAG
ACCTTTTTTCGACGTGAATATCAAGAATTTTCTTCTATCTCACTTCATAG
ACCTTTTCAACAAGGTTTTAAAAATGAAATTCGAGCAGGTATCTCCTGAT
GAATTTGCTGAACTTTGCAAAGTATACAGGGCGTTATGTATTGAGTGCGC
AACAGACGACACCTTTAATAGTAATAGTGATTTGATCGCCGCAAAAGATG
CGTTTTTGGTATCTGTGTTGAGAATCGCAGATGGGTTTTGGGAGCATGAC
AAACTTTTGCAGCTAAGGATGCTTGATAGTAATATGAACATACCTAATCA
GATTCCTCATACTACTTTGCAAAGTTCGCTATCTGCGATTGTAATAAAGA
TAATAGAAAGTAATATAGGAAAAATTGAGGCGTCTGAACCTTTCAAAACC
TTCAAGAATACGCACCATCACCATCACCATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDVDEPNPIVISDSEATDE
EISIIYEPEFNENYLWAEENVQEASRSQKIVTERLSLDSTAGESCTPSVV
TDTQVTTGLRWSLRKRKAIQKMPYSLERIKHRQLLEGYDISSFDSISNQL
TLPKNASTVIHSNDILLTKRTGKPLDEQKDVTIDSIKPENSSVQSQRYDS
DEEIPKKRHRTFKDLDQDIVFQSGDSTEDEQDLASTNLQNTQNDEVIFRG
RVLNVRTGYRGVLPRVAWEKSLQKQQSSKVTKRKTQLLNHKGVAKRKMNR
SAHIEDEEQNLLNDLIAPDDELDIEENAPPDIYLGNLPEDREANEKELKE
LQEYYESKYSEDAQSAGTSGFNLNEEYRNEPVYELEYDGPGSCISHVSYK
DQPIIYLNSRHSDSGFSEQYNISAEDNQSVISLDAAEEHNDGIIDKMLVK
PKRIKATNDANFLNTKSKRVRRYKYKYRNSCLAPSTKAIKVGKRSAHKSH
LAANNPVSFVSKKNHVIDDYFFEELESQSLEQDDSSSLKPQKKRRKKKAP
IYSSFSADLESRRKPVFNTVVEVPTNRYAFTKPNVRNRDSINHDMEFEEE
DSNQELGPIMVVLDSILLKKPFEPPNFFKIQLSDKSFLLSKLNPADIATS
LQKIFRVIIDKGITDTELVHFNESLIAFLVHLDMPELFDLIGEFHREFRS
KVNSLRKKAKPIHFFQIAACQLMFLEISRYNKISAAAKFDMDVKLLDHIV
SFFKLLSVCYDSVMKNPMQYLYTSYYILSAVVDVIHKKEALWDLFQKHPF
SPHISLLLVNIFPTKVCRWQVLRLDSEFQPLSSAFRFINYCIETCNWNVT
NSLILSLDRIFKRRRFSDFEEESDLSQNNKIIYPPTNQLTSRLMFNRYLH
LLTLCELSSSDTQRVIPMGDISMNDSLSVLKNRLNLLIVLATRFDLNLEK
RFQELTRPLYSKEYLNLHTQNTVRTITTLIMQASLSFLEISRIKNHPFSG
KFIASLFDKLVLQQPSISGVTENFLKEFTNLVSKMKRKSVSMLKFLYPSL
VAMSQENIFESSFFLLLQVYLKSLDVLGPTWVQNYLFQFIKSKAQENERW
IECYCQIGKFLVDSGIFTWWTFFTYNGLDAALHFQLAFHSLIIDFCDTDS
FELLKKPLYSIASDLLLISKDDAFYHFLSNLLKRAHIIVADLKPVSDENE
LLRLAYIFSKALKKNAYQDLLAVFLSLAKKHYDEGDISRNFLAKYLEFLN
KNCLTELRNNQLFISLRRELGISSDEDEKCAFWDSFNEAGDILSKAAFVE
TGIVQACCTGNEIDGYLDNLSTLFTSTMLESPFAFFSDLVIAHIFENRPF
FDVNIKNFLLSHFIDLFNKVLKMKFEQVSPDEFAELCKVYRALCIECATD
DTFNSNSDLIAAKDAFLVSVLRIADGFWEHDKLLQLRMLDSNMNIPNQIP
HTTLQSSLSAIVIKIIESNIGKIEASEPFKTFKNTHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11362
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 195325 Daltons