cDNA Clone CNOT7

• Plasmid: pEBG6P-1 CNOT7 long version
• Parent Plasmid: pEBG6P
• Expressed: GST-CNOT7 (long)
• Genbank: NM_013354
• Tag: GST
• Species: Human

Sequence of Insert

ggatccatgccagcggcaactgtagatcatagccaaagaatttgtgaagtttgggcttgcaacttggatgaagagatgaagaaaattcgtcaagttatccgaaaatataattacgttgctatggacaccgagtttccaggtgtggttgcaagacccattggagaattcaggagcaatgctgactatcaataccaactattgcggtgtaatgtagacttgttaaagataattcagctaggactgacatttatgaatgagcaaggagaataccctccaggaacttcaacttggcagtttaattttaaa
tttaatttgacggaggacatgtatgcccaggactctatagagctactaac
aacatctggtatccagtttaaaaaacatgaggaggaaggaattgaaaccc
agtactttgcagaacttcttatgacttctggagtggtcctctgtgaaggg
gtcaaatggttgtcatttcatagcggttacgactttggctacttaatcaa
aatcctaaccaactctaacttgcctgaagaagaacttgacttctttgaga
tccttcgattgttttttcctgtcatttatgatgtgaagtacctcatgaag
agctgcaaaaatctcaaaggtggattacaggaggtggcagaacagttaga
gctggaacggataggaccacaacatcaggcaggatctgattcattgctca
caggaatggcctttttcaaaatgagagaaatgttctttgaagatcatatt
gatgatgccaaatattgtggtcatttgtatggccttggttctggttcatc
ctatgtacagaatggcacagggaatgcatatgaagaggaagccaacaagc
agtcatgagcggccgc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMPAATVDHSQRIC
EVWACNLDEEMKKIRQVIRKYNYVAMDTEFPGVVARPIGEFRSNADYQYQ
LLRCNVDLLKIIQLGLTFMNEQGEYPPGTSTWQFNFKFNLTEDMYAQDSI
ELLTTSGIQFKKHEEEGIETQYFAELLMTSGVVLCEGVKWLSFHSGYDFG
YLIKILTNSNLPEEELDFFEILRLFFPVIYDVKYLMKSCKNLKGGLQEVA
EQLELERIGPQHQAGSDSLLTGMAFFKMREMFFEDHIDDAKYCGHLYGLG
SGSSYVQNGTGNAYEEEANKQS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11248
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express CNOT7 long variant with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 60161 Daltons