cDNA Clone TNF alpha (mouse)

• Plasmid: pCMV5 TNFalpha-GFP deletion in TNFalpha 3′UTR
• Parent Plasmid: pCMV5
• Tag: GFP

Sequence of Insert

atcgatAAGCTTCCTCAGCGAGGACAGCAAGGGACTAGCCAGGAGGGgGAACAGAAACTCCAGAACATCCTGGAAATAGCTCCCAGAAAAGCAAGCAGCCAACCAGGCAGGTTCTGTCCCTTTCACTCACTGGCCCAAGGCGCCACATCTCCCTCCAGAAAAGACACCATGGTGAGCAAGGGCaAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAG
TTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGAC
CACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGA
AGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAG
CGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGT
GAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCG
ACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTAC
AACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAA
GGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCG
CCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTG
CCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAA
CGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGA
TCACTCTCGGCATGGACGAGCTGTACAAGATGAGCACAGAAAGCATGATC
CGCGACGTGGAACTaGCAGAAGAGGCACTCCCCCAAAAGgTGGGGGGCTT
CCAGAACTCCAGGCGGTGCCTATGTCTCAGCCTCTTCTCATTCCTGCTTG
TGGCAGGGGCCACCACGCTCTTCTGTCTACTGAACTTCGGGGTGATCGGT
CCCCAAAGGGATGAGAAGTTCCCAAATGGCCTCCCTCTCATCAGTTCTAT
GGCCCAGACCCTCACACTCAGATCATCTTCTCAAAATTCGAGTGACAAGC
CTGTAGCCCACGTCGTAGCAAACCACCAAGTGGAGGAGCAGCTGGAGTGG
CTGAGCCAGCGCGCCAACGCCCTCCTGGCCAACGGCATGGATCTCAAAGA
CAACCAACTAGTGGTGCCAGCCGATGGGTTGTACCTTGTCTACTCCCAGG
TTCTCTTCAAGGGACAAGGCTGCCCCGACTACGTGCTCCTCACCCACACC
GTCAGCCGATTTGCTATCTCATACCAGGAGAAAGTCAACCTCCTCTCTGC
CGTCAAGAGCCCCTGCCCCAAGGACACCCCTGAGGGGGCTGAGCTCAAAC
CCTGGTATGAGCCCATATACCTGGGAGGAGTCTTCCAGCTGGAGAAGGGG
GACCAACTCAGCGCTGAGGTCAgTCTGCCCAAGTACTTAGACTTTGCGGA
GTCCGGGCAGGTCTACTTTGGAGTCATTGCTCTGTGAAGGGAATGGGTGT
TCATCCATTCTCTACCCAGCCCCCACTCTGACCCCTTTACTCTGACCCCg
gccgCCCAATTGTCTTAATAACGCTGATTTGGTGACCAGGCTGTCGCTAC
ATCACTGAACCTCTGCTCCCCACGGGAGCCGTGACTGTAATTGCCCTACA
GTCAATTGAGAGAAAaagggcgaattctgcagatatccatcacactggca
gccgctcgag

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5892
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *DU245 (mutagenesis to create Eag1 sites to make deletion in 3’UTR, then corrective mutagenesis to correct framshift mutation inherited from DU245)