cDNA Clone MNK1

• Synonyms: MKNK1
• Plasmid: pFastBacHTb MNK1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-MNK1
• Genbank: AB000409
• Tag: 6His

Sequence of Insert

GGATCCATGGTATCTTCTCAAAAGTTGGAAAAACCTATAGAGATGGGCAGTAGCGAACCCCTTCCCATCGCAGATGGTGACAGGAGGAGGAAGAAGAAGCGGAGGGGCCGGGCCACTGACTCCTTGCCAGGAAAGTTTGAAGATATGTACAAGCTGACCTCTGAATTGCTTGGAGAGGGAGCCTATGCCAAAGTTCAAGGTGCCGTGAGCCTACAGAATGGCAAAGAGTATGCCGTCAAAATCATCGAGAAACAAGCAGGGCACAGTCGGAGTAGGGTGTTTCGAGAGGTGGAGACGCTGTATCAG
TGTCAGGGAAACAAGAACATTTTGGAGCTGATTGAGTTCTTTGAAGATGA
CACAAGGTTTTACTTGGTCTTTGAGAAATTGCAAGGAGGTTCCATCTTAG
CCCACATCCAGAAGCAAAAGCACTTCAATGAGCGAGAAGCCAGCCGAGTG
GTGCGGGACGTTGCTGCTGCCCTTGACTTCCTGCATACCAAAGGCATTGC
TCATCGTGATCTGAAACCAGAAAATATATTGTGTGAATCTCCAGAAAAGG
TGTCTCCAGTGAAAATCTGTGACTTTGACTTGGGCAGTGGGATGAAACTG
AACAACTCCTGTACCCCCATAACCACACCAGAGCTGACCACCCCATGTGG
CTCTGCAGAATACATGGCCCCTGAGGTAGTGGAGGTCTTCACGGACCAGG
CCACATTCTACGACAAGCGCTGTGACCTGTGGAGCCTGGGCGTGGTCCTC
TACATCATGCTGAGTGGCTACCCACCCTTCGTGGGTCACTGCGGGGCCGA
CTGTGGCTGGGACCGGGGCGAGGTCTGCAGGGTGTGCCAGAACAAGCTGT
TTGAAAGCATCCAGGAAGGCAAGTATGAGTTTCCTGACAAGGACTGGGCA
CACATCTCCAGTGAAGCCAAAGACCTCATCTCCAAGCTCCTGGTGCGAGA
TGCAAAGCAGAGACTTAGCGCCGCCCAAGTTCTGCAGCACCCATGGGTGC
AGGGGCAAGCTCCAGAAAAGGGACTCCCCACGCCGCAAGTCCTCCAGAGG
AACAGCAGCACAATGGACCTGACGCTCTTCGCAGCTGAGGCCATCGCCCT
TAACCGCCAGCTATCTCAGCACGAAGAGAACGAACTAGCAGAGGAGCCAG
AGGCACTAGCTGATGGCCTCTGCTCCATGAAGCTTTCCCCTCCCTGCAAG
TCACGCCTGGCCCGGAGACGGGCCCTGGCCCAGGCAGGCCGTGGTGAAGA
CAGGAGCCCGCCCACAGCACTCTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMVSSQKLEKPIEMGSSEPLPIA
DGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNG
KEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYL
VFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKGIAHRDLK
PENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYM
APEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDR
GEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRL
SAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLS
QHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPT
AL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5872
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 50771 Daltons