cDNA Clone TNF alpha (mouse)

• Plasmid: pCMV5 GFP-TNF alpha deletion mutant (needs correcting)
• Parent Plasmid: pCMV5 C-GFP
• Genbank: X02611
• Tag: GFP
• Species: Mouse

Sequence of Insert

AAGCTTCCTCAGCGAGGACAGCAAGGGACTAGCCAGGAGGGGGAACAGAA
ACTCCAGAACATCCTGGAAATAGCTCCCAGAAAAGCAAGCAGCCAACCAG
GCAGGTTCTGTCCCTTTCACTCACTGGCCCAAGGCGCCACATCTCCCTCC
AGAAAAGACACCATGGTGAGCAAGGGCAAGGAGCTGTTCACCGGGGTGGT
GCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCG
TGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAG
TTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGAC
CACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGA
AGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAG
CGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGT
GAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCG
ACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTAC
AACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAA
GGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCG
CCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTG
CCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAA
CGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGA
TCACTCTCGGCATGGACGAGCTGTACAAGATGAGCACAGAAAGCATGAT
CCGCGACGTGGAACTAGCAGAAGAGGCACTCCCCCAAAAGGTGGGGGGCT
TCCAGAACTCCAGGCGGTGCCTATGTCTCAGCCTCTTCTCATTCCTGCTT
GTGGCAGGGGCCACCACGCTCTTCTGTCTACTGAACTTCGGGGTGATCGG
TCCCCAAAGGGATGAGAAGTTCCCAAATGGCCTCCCTCTCATCAGTTCTA
TGGCCCAGACCCTCACACTCAGATCATCTTCTCAAAATTCGAGTGACAAG
CCTGTAGCCCACGTCGTAGCAAACCACCAAGTGGAGGAGCAGCTGGAGTG
GCTGAGCCAGCGCGCCAACGCCCTCCTGGCCAACGGCATGGATCTCAAAG
ACAACCAACTAGTGGTGCCAGCCGATGGGTTGTACCTTGTCTACTCCCAG
GTTCTCTTCAAGGGACAAGGCTGCCCCGACTACGTGCTCCTCACCCACAC
CGTCAGCCGATTTGCTATCTCATACCAGGAGAAAGTCAACCTCCTCTCTG
CCGTCAAGAGCCCCTGCCCCAAGGACACCCCTGAGGGGGCTGAGCTCAAA
CCCTGGTATGAGCCCATATACCTGGGAGGAGTCTTCCAGCTGGAGAAGGG
GGACCAACTCAGCGCTGAGGTCAGTCTGCCCAAGTACTTAGACTTTGCGG
AGTCCGGGCAGGTCTACTTTGGAGTCATTGCTCTGTGAAGGGAATGGGTG
TTCATCCATTCTCTACCCAGCCCCCACTCTGACCCCTTTACTCTGACCCC
GGCCGCCCAATTGTCTTAATAACGCTGATTTGGTGACCAGGCTGTCGCTA
CATCACTGAACCTCTGCTCCCCACGGGAGCCGTGACTGTAATTGCCCTAC
AGTCAATTGAGAGAAAAAGGGCGAATTCTGCAGATATCCATCACACTGGC
AGCCG

Amino Acid Sequence

MVSKGKELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKMSTESMIRDVE
LAEEALPQKVGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRD
EKFPNGLPLISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQR
ANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRF
AISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLS
AEVSLPKYLDFAESGQVYFGVIAL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5868
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Mutagenesis to introduce two Eag1 sites flanking sequence to be deleted (and mutagenesis to remove Eag1 site at 3’ end of insert in DU245), then removal of sequence to be deleted and re-ligation.; ; Mammalian construct to express 5'UTR Tnfalpha-GFP-Tnfalpha-3'UTR Tnfalpha with deletion - to be used as a reporter