cDNA Clone 14-3-3 zeta

• Synonyms: YWHAZ
• Plasmid: pGEX6P-1-14-3-3 zeta R56A R60A
• Parent Plasmid: pGEX6P1
• Expressed: GST-14-3-3 zeta R56A R60A
• Genbank: NM_145690
• Tag: GST
• Species: Human

Sequence of Insert

agatctGATAAAAATGAGCTGGTTCAGAAGGCCAAACTGGCCGAGCAGGCTGAGCGATATGATGACATGGCAGCCTGCATGAAGTCTGTAACTGAGCAAGGAGCTGAATTATCCAATGAGGAGAGGAATCTTCTCTCAGTTGCTTATAAAAAT
GTTGTAGGAGCCCGTGCGTCATCTTGGGCGGTCGTCTCAAGTATTGAACA
AAAGACGGAAGGTGCTGAGAAAAAACAGCAGATGGCTCGAGAATACAGAG
AGAAAATTGAGACGGAGCTAAGAGATATCTGCAATGATGTACTGTCTCTT
TTGGAAAAGTTCTTGATCCCCAATGCTTCACAAGCAGAGAGCAAAGTCTT
CTATTTGAAAATGAAAGGAGATTACTACCGTTACTTGGCTGAGGTTGCCG
CTGGTGATGACAAGAAAGGGATTGTCGATCAGTCACAACAAGCATACCAA
GAAGCTTTTGAAATCAGCAAAAAGGAAATGCAACCAACACATCCTATCAG
ACTGGGTCTGGCCCTTAACTTCTCTGTGTTCTATTATGAGATTCTGAACT
CCCCAGAGAAAGCCTGCTCTCTTGCAAAGACAGCTTTTGATGAAGCCATT
GCTGAACTTGATACATTAAGTGAAGAGTCATACAAAGACAGCACGCTAAT
AATGCAATTACTGAGAGACAACTTGACATTGTGGACATCGGATACCCAAG
GAGACGAAGCTGAAGCAGGAGAAGGAGGGGAAAATTAAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSDKNELVQKAKLAEQAERYD
DMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARASSWAVVSSIEQKTE
GAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLK
MKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGL
ALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQL
LRDNLTLWTSDTQGDEAEAGEGGEN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5827
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 54265 Daltons