cDNA Clone FUS

• Plasmid: pGEX6P-1-FUS 1-374
• Parent Plasmid: pGEX6P1
• Expressed: GST-FUS 1-374
• Genbank: NM_004960
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCCTCAAACGATTATACCCAACAAGCAACCCAAAGCTATGGGGCCTACCCCACCCAGCCCGGGCAGGGCTATTCCCAGCAGAGCAGTCAGCCCTACGGACAGCAGAGTTACAGTGGTTATAGCCAGTCCACGGACACTTCAGGCTATGGCCAGAGCAGCTATTCTTCTTATGGCCAGAGCCAGAACACAGGCTATGGAACTCAGTCAACTCCCCAGGGATATGGCTCGACTGGCGGCTATGGCAGTAGCCAGAGCTCCCAATCGTCTTACGGGCAGCAGTCCTCCTATCCTGGCTAT
GGCCAGCAGCCAGCTCCCAGCAGCACCTCGGGAAGTTACGGTAGCAGTTC
TCAGAGCAGCAGCTATGGGCAGCCCCAGAGTGGGAGCTACAGCCAGCAGC
CTAGCTATGGTGGACAGCAGCAAAGCTATGGACAGCAGCAAAGCTATAAT
CCCCCTCAGGGCTATGGACAGCAGAACCAGTACAACAGCAGCAGTGGTGG
TGGAGGTGGAGGTGGAGGTGGAGGTAACTATGGCCAAGATCAATCCTCCA
TGAGTAGTGGTGGTGGCAGTGGTGGCGGTTATGGCAATCAAGACCAGAGT
GGTGGAGGTGGCAGCGGTGGCTATGGACAGCAGGACCGTGGAGGCCGCGG
CAGGGGTGGCAGTGGTGGCGGCGGCGGCGGCGGCGGTGGTGGTTACAACC
GCAGCAGTGGTGGCTATGAACCCAGAGGTCGTGGAGGTGGCCGTGGAGGC
AGAGGTGGCATGGGCGGAAGTGACCGTGGTGGCTTCAATAAATTTGGTGG
CCCTCGGGACCAAGGATCACGTCATGACTCCGAACAGGATAATTCAGACA
ACAACACCATCTTTGTGCAAGGCCTGGGTGAGAATGTTACAATTGAGTCT
GTGGCTGATTACTTCAAGCAGATTGGTATTATTAAGACAAACAAGAAAAC
GGGACAGCCCATGATTAATTTGTACACAGACAGGGAAACTGGCAAGCTGA
AGGGAGAGGCAACGGTCTCTTTTGATGACCCACCTTCAGCTAAAGCAGCT
ATTGACTGGTTTGATGGTAAAGAATTCTCCGGAAATCCTATCAAGGTCTC
ATTTGCTACTCGCCGGGCAGACTAGAATCGGGGTGGTGGCAATGGTCGTG
GAGGCCGAGGGCGAGGAGGACCCATGGGCCGTGGAGGCTATGGAGGTGGT
GGCAGTGGTGGTGGTGGCCGAGGAGGATTTCCCAGTGGAGGTGGTGGCGG
TGGAGGACAGCAGCGAGCTGGTGACTGGAAGTGTCCTAATCCCACCTGTG
AGAATATGAACTTCTCTTGGAGGAATGAATGCAACCAGTGTAAGGCCCCT
AAACCAGATGGCCCAGGAGGGGGACCAGGTGGCTCTCACATGGGGGGTAA
CTACGGGGATGATCGTCGTGGTGGCAGAGGAGGCTATGATCGAGGCGGCT
ACCGGGGCCGCGGCGGGGACCGTGGAGGCTTCCGAGGGGGCCGGGGTGGT
GGGGACAGAGGTGGCTTTGGCCCTGGCAAGATGGATTCCAGGGGTGAGCA
CAGACAGGATCGCAGGGAGAGGCCGTATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMASNDYTQQATQSYGAYPT
QPGQGYSQQSSQPYGQQSYSGYSQSTDTSGYGQSSYSSYGQSQNTGYGTQ
STPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPAPSSTSGSYGSSSQS
SSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSSSGGGG
GGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGGRGRG
GSGGGGGGGGGGYNRSSGGYEPRGRGGGRGGRGGMGGSDRGGFNKFGGPR
DQGSRHDSEQDNSDNNTIFVQGLGENVTIESVADYFKQIGIIKTNKKTGQ
PMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFSGNPIKVSFA
TRRAD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5822
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. sequence difference compared with FUS NM_004960 CDS:; c291t (silent Y97); ; Estimated MW of expressed protein : 64957 Daltons