cDNA Clone 14-3-3 zeta

• Synonyms: YWHAZ
• Plasmid: pCMV5-HA 14-3-3 zeta R56A / R60A mutant
• Parent Plasmid: pCMV5
• Expressed: HA-14-3-3 zeta R56A / R60A mutant
• Genbank: NM_145690 (wild type)
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGATAAAAA
TGAGCTGGTTCAGAAGGCCAAACTGGCCGAGCAGGCTGAGCGATATGATG
ACATGGCAGCCTGCATGAAGTCTGTAACTGAGCAAGGAGCTGAATTATCC
AATGAGGAGAGGAATCTTCTCTCAGTTGCTTATAAAAATGTTGTAGGAGC
CCGTGCGTCATCTTGGGCGGTCGTCTCAAGTATTGAACAAAAGACGGAAG
GTGCTGAGAAAAAACAGCAGATGGCTCGAGAATACAGAGAGAAAATTGAG
ACGGAGCTAAGAGATATCTGCAATGATGTACTGTCTCTTTTGGAAAAGTT
CTTGATCCCCAATGCTTCACAAGCAGAGAGCAAAGTCTTCTATTTGAAAA
TGAAAGGAGATTACTACCGTTACTTGGCTGAGGTTGCCGCTGGTGATGAC
AAGAAAGGGATTGTCGATCAGTCACAACAAGCATACCAAGAAGCTTTTGA
AATCAGCAAAAAGGAAATGCAACCAACACATCCTATCAGACTGGGTCTGG
CCCTTAACTTCTCTGTGTTCTATTATGAGATTCTGAACTCCCCAGAGAAA
GCCTGCTCTCTTGCAAAGACAGCTTTTGATGAAGCCATTGCTGAACTTGA
TACATTAAGTGAAGAGTCATACAAAGACAGCACGCTAATAATGCAATTAC
TGAGAGACAACTTGACATTGTGGACATCGGATACCCAAGGAGACGAAGCT
GAAGCAGGAGAAGGAGGGGAAAATTAAGGATCC

Amino Acid Sequence

MYPYDVPDYADKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEER
NLLSVAYKNVVGARASSWAVVSSIEQKTEGAEKKQQMAREYREKIETELR
DICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGI
VDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSL
AKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGE
GGEN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5798
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression construct to express HA N-terminal fusion.