cDNA Clone Neomycin

• Plasmid: pBluescript-II-KS+ FRT-neo/EpCMV IRES
• Parent Plasmid: pBluescript frt neo

Sequence of Insert

DNA sequence of loxp-neo insert :GCGGCCGCGGCCGGCCGAAGTTCCTATTCCGAAGTTCCTATTCTCTAGAA
AGTATAGGAACTTCTTAGGTCTGAAGAGGAGTTTACGTCCAGCCAAGCTA
GCTTGGCTGCAGGTCGAGCAGTGTGGTTTTGCAAGAGGAAGCAAAAAGCC
TCTCCACCCAGGCCTGGAATGTTTCCACCCAATGTCGAGCAGTGTGGTTT
TGCAAGAGGAAGCAAAAAGCCTCTCCACCCAGGCCTGGAATGTTTCCACC
CAATGTCGAGCAAACCCCGCCCAGCGTCTTGTCATTGGCGAATTCGAACA
CGCAGATGCAGTCGGGGCGGCGCGGTCCCAGGTCCACTTCGCATATTAAG
GTGACGCGTGTGGCCTCGAACACCGAGCGACCCTGCAGCCAATATGGGAT
CGGCCATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTG
GAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGA
TGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCA
AGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGG
CTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGT
TGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGC
AGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATG
GCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATT
CGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAG
CCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCG
CCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGA
TCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAA
ATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGAC
CGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGG
CGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCG
ATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGACCA
CGCGTCGGGTCGACGGCGCGCCTCGAGTT

DNA sequence of ECMV IRES insert :TCTCGAGATAACTAAGTTTAAACTCCGCCCCTCTCCCTCCCCCCCCCCTA
ACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTAT
ATGTGATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAA
ACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCG
CCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTG
GAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCG
GAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTAT
AAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGG
ATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGG
GCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGC
CTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTA
GGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATA
ATATCGCCACAACCGGTGGCGTTTAATTAAGAAGTTCCTATTCCGAAGTT
CCTATTCTCTAGAAAGTATAGGAACTTCGGCGCGCCA

• Vector Type: Other
• Antibiotic: Amp
• Full Snapgene Map: pBluescript-II-KS+ FRT-neo/EpCMV IRES63.47 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU5788
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: PolyA trap vector for Simon Arthur.
• Low Temp: No