cDNA Clone MP2

• Plasmid: pNTAP MP2
• Parent Plasmid: pNTAP-B (Stratagene)
• Expressed: Homo sapiens mini-protein2 (MP2) Hdm2 (double minute 2). CDS nt 320-621 with N-terminal TAP tag and artificial Stop
• Species: Human

Sequence of Insert

GTAGTAGTCAATCAGCAGGAATCATCGGACTCAGGTACATCTGTGAGTGA
GAACAGGTGTCACCTTGAAGGTGGGAGTGATCAAAAGGACCTTGTACAAG

AGCTTCAGGAAGAGAAACCTTCATCTTCACATTTGGTTTCTAGACCATCT

ACCTCATCTAGAAGGAGAGCAATTAGTGAGACAGAAGAAAATTCAGATGA

ATTATCTGGTGAACGACAAAGAAAACGCCACAAATCTGATAGTATTTCCC

TTTCCTTTGATGAAAGCCTGGCTCTGTGTGTAATAAGGGAGATATGTTGT

TAG

Amino Acid Sequence

MKRRWKKNFIAVSAANRFKKISSSGALGSGSGTMDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQCQREPSGGCKLGCPGGSVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICC*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU5732
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression construct to express Tandem affinity purification (TAP) N-terminal fusion. (CBP and SBP tags )