cDNA Clone FGD6

• Plasmid: pCMV5-FLAG FGD6
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG-FGD6
• Genbank: NM_018351
• Tag: FLAG
• Species: Human

Sequence of Insert

gcggccgcgatgacttctgcagccgagataaagaagccaccagtggcccccaagcccaagtttgttgtggcaaataacaagccagccccacctcctattgcacctaaacccgacattgtgatttctagtgttccacagtcgacaaagaaaatgaaaccagcaatagccccaaaaccaaaagtcctgaagacctcacctgttcgagagattgggcagtcgccatcaaggaaaatcatgttgaacctggaagggcataaacaggaattagctgaaagcactgacaactttaattgtaaatatgaaggcaatcagagcaatgattatatttcaccaatgtgttcctgcagttctgagtgtatccataagctgggccatagagagaatttgtgtgtaaagcagcttgtcttagagcccctggaaatgaatgaaaatttagaaaacagtaaaattgatgagact
ttgactataaaaactaggagtaaatgtgatttgtatggtgaaaaagccaa
gaaccagggtggagttgttttaaaggcaagcgttttagaagaggagctca
aagatgccttaatacaccaaatgccaccttttatttctgcacagaagcac
aggcccacagacagcccagaaatgaatggtggctgtaattcaaatggaca
attcagaattgaatttgcggatttgtcaccttccccatccagctttgaaa
aagttcctgatcatcacagttgccacttacagcttcctagtgatgaatgt
gaacattttgaaacttgccaggatgacagtgaaaaaagcaataattgctt
tcagtcatctgaactagaggctctggaaaatgggaaaaggagtactttaa
tatcttcagatggagttagtaagaaatcagaagtcaaagaccttggtccc
ttagaaattcatttagtaccatataccccaaaatttccaactcccaagcc
cagaaagacacgaactgctcgtctgttacgccaaaagtgtgtagatactc
ctagtgaaagcactgaagaaccggggaattcagacagtagctcttcctgt
cttactgaaaatagtttgaaaatcaataaaatcagtgttctgcatcagaa
tgttttgtgtaagcaggaacaggtggataaaatgaagctaggaaataaaa
gtgaattgaatatggaatccaacagtgatgcacaggacttagtcaattca
cagaaagccatgtgtaatgaaacaacttcctttgaaaaaatggcaccttc
ttttgataaagactctaatttgagttctgacagcacaactgtagatggtt
ctagtatgtcgcttgctgtggacgaagggaccggttttataagatgtact
gtatctatgagcctgcctaagcagctcaaattaacttgcaatgaacattt
gcaatctgggagaaacctgggagtttctgcccctcaaatgcaaaaggaat
ctgttataaaagaggaaaattctctacgaattgtccccaaaaaacctcaa
agacatagcttgcctgctacaggagtgcttaaaaaggctgcctccgagga
gcttttggaaaaaagttcttatccttcaagtgaagaaaaaagttcagaga
agagtctagaaagaaatcaccttcagcatttgtgtgcccaaaaccgtggt
gtgtcatcctcctttgatatgcctaaacgggcttcagaaaagccagtgtg
gaagttacctcatcctattttacccttttcagggaacccagaattcttaa
agtctgtcaccgtatcgtcaaacagtgagccttcaacagccctaaccaag
cccagagcaaaatcgttatctgctatggatgtggaaaagtgcactaagcc
ttgcaaagactctacaaagaaaaactcttttaaaaagttgctcagcatga
aactgtccatctgtttcatgaagagtgactttcaaaaattttggtccaag
agtagccaacttggagacaccaccacaggccacctctccagtggggagca
gaaggggattgaaagtgattggcaaggcttgttggtaggagaggagaaga
gaagtaaacccatcaaggcatattccacagaaaactatagcctggaatct
caaaagaagaggaagaagtctcggggccagaccagtgcagctaatggtct
gagagctgagtctttggatgaccaaatgctctcccgggagtcatcatctc
aggcaccttacaagtctgttacaagcctctgtgcaccggagtatgaaaat
atacgccattatgaggaaataccagagtacgagaacttgccatttattat
ggctatacggaaaactcaagagttggaatggcagaattccagcagcatgg
aggacgctgatgcaaatgtgtatgaggtagaagagccgtatgaagctcca
gatggccagctgcagcttggacccagacatcagcattccagttcaggagc
atcccaggaggaacagaatgatcttggtcttggtgaccttccctctgatg
aggaggaaatcatcaacagttctgatgaagatgatgtcagctctgagtca
agtaaaggagagcctgacccactggaagataaacaggatgaagataatgg
aatgaaaagtaaagttcatcatattgccaaggagatcatgagctcagaga
aagtgtttgtggatgtgttaaaacttttgcatattgatttccgggatgca
gtagctcatgcttccaggcaacttgggaaaccagtgattgaggaccggat
tctaaatcagatcctatactacttgcctcagctgtatgagctcaaccggg
atctcttgaaggaactggaggaaagaatgttgcactggactgaacaccaa
agaattgctgatatctttgtaaagaagggaccatatctaaaaatgtattc
cacatacatcaaagaatttgataagaatatagccttgctggatgaacagt
gcaagaaaaatccaggttttgctgctgttgttagagaatttgagatgagc
cctcgctgtgctaatctggccctcaagcactacctgctcaagccggttca
gaggatcccccagtacaggctgttgctgacagattatttgaagaatctca
tagaagatgctggagattacagagacactcaagatgcccttgctgttgtt
atagaggtagccaaccacgccaatgacaccatgaagcaaggagacaactt
tcagaaacttatgcaaattcagtacagcttaaatggacaccatgaaattg
tgcagcctggtcgggtttttctcaaagaaggaattctgatgaagctgtct
cggaaagtgatgcaacctcgaatgtttttcctgtttaatgatgccctgct
gtatacaacaccagtgcagtctgggatgtataaactgaacaacatgctct
cactggctggaatgaaggtcagaaaacctacccaagaagcctatcagaat
gaattaaagattgaaagtgtagaacgttccttcattctctcagccagttc
tgccacagaaagggatgaatggctagaagcgatttccagggcaatagaag
agtatgccaagaaaagaatcaccttctgtcctagtaggagtcttgatgag
gcagactcagaaaataaagaagaagttagtcctcttggatcgaaggctcc
catctggattcctgataccagagccacaatgtgtatgatctgcacaagcg
aattcactctcacctggagacgacaccactgccgggcctgtggaaagatt
gtatgccaagcttgttcgtctaataagtatggcttagattacctgaaaaa
tcaaccagcaagagtatgtgaacattgtttccaagaactgcagaaattag
atcaccagcactcccctaggattggatctcctggaaatcacaaatctcct
tcaagtgccttatcatcagtcttacatagcattccatcagggaggaaaca
gaaaaaaatcccagctgctctcaaagaagtatcagcaaacacagaggatt
cttctatgagtggctacttgtacagatcaaagggcaataaaaaaccctgg
aaacacttttggtttgtcataaaaaataaagtactatatacatatgctgc
aagtgaggacgtggccgctttggagagtcagcctttattaggattcactg
ttattcaagttaaagatgagaattccgagtctaaagtatttcagttactg
cacaaaaacatgttattttatgtattcaaagcagaggatgctcactcggc
tcagaagtggatagaagcatttcaggaaggcacaatattgtaggcggccgc

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMTSAAEIKKPPVAPKPKFVVANNKPAPP
PIAPKPDIVISSVPQSTKKMKPAIAPKPKVLKTSPVREIGQSPSRKIMLN
LEGHKQELAESTDNFNCKYEGNQSNDYISPMCSCSSECIHKLGHRENLCV
KQLVLEPLEMNENLENSKIDETLTIKTRSKCDLYGEKAKNQGGVVLKASV
LEEELKDALIHQMPPFISAQKHRPTDSPEMNGGCNSNGQFRIEFADLSPS
PSSFEKVPDHHSCHLQLPSDECEHFETCQDDSEKSNNCFQSSELEALENG
KRSTLISSDGVSKKSEVKDLGPLEIHLVPYTPKFPTPKPRKTRTARLLRQ
KCVDTPSESTEEPGNSDSSSSCLTENSLKINKISVLHQNVLCKQEQVDKM
KLGNKSELNMESNSDAQDLVNSQKAMCNETTSFEKMAPSFDKDSNLSSDS
TTVDGSSMSLAVDEGTGFIRCTVSMSLPKQLKLTCNEHLQSGRNLGVSAP
QMQKESVIKEENSLRIVPKKPQRHSLPATGVLKKAASEELLEKSSYPSSE
EKSSEKSLERNHLQHLCAQNRGVSSSFDMPKRASEKPVWKLPHPILPFSG
NPEFLKSVTVSSNSEPSTALTKPRAKSLSAMDVEKCTKPCKDSTKKNSFK
KLLSMKLSICFMKSDFQKFWSKSSQLGDTTTGHLSSGEQKGIESDWQGLL
VGEEKRSKPIKAYSTENYSLESQKKRKKSRGQTSAANGLRAESLDDQMLS
RESSSQAPYKSVTSLCAPEYENIRHYEEIPEYENLPFIMAIRKTQELEWQ
NSSSMEDADANVYEVEEPYEAPDGQLQLGPRHQHSSSGASQEEQNDLGLG
DLPSDEEEIINSSDEDDVSSESSKGEPDPLEDKQDEDNGMKSKVHHIAKE
IMSSEKVFVDVLKLLHIDFRDAVAHASRQLGKPVIEDRILNQILYYLPQL
YELNRDLLKELEERMLHWTEHQRIADIFVKKGPYLKMYSTYIKEFDKNIA
LLDEQCKKNPGFAAVVREFEMSPRCANLALKHYLLKPVQRIPQYRLLLTD
YLKNLIEDAGDYRDTQDALAVVIEVANHANDTMKQGDNFQKLMQIQYSLN
GHHEIVQPGRVFLKEGILMKLSRKVMQPRMFFLFNDALLYTTPVQSGMYK
LNNMLSLAGMKVRKPTQEAYQNELKIESVERSFILSASSATERDEWLEAI
SRAIEEYAKKRITFCPSRSLDEADSENKEEVSPLGSKAPIWIPDTRATMC
MICTSEFTLTWRRHHCRACGKIVCQACSSNKYGLDYLKNQPARVCEHCFQ
ELQKLDHQHSPRIGSPGNHKSPSSALSSVLHSIPSGRKQKKIPAALKEVS
ANTEDSSMSGYLYRSKGNKKPWKHFWFVIKNKVLYTYAASEDVAALESQP
LLGFTVIQVKDENSESKVFQLLHKNMLFYVFKAEDAHSAQKWIEAFQEGT
IL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5613
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. sequence differences (all silent) compared with NM_018351:; t69c(N23), t396c(V132), g513a(G171), c1962t(L654), a2388g(P796), t2847c(H949), g2850a(Q950), t4245c(H1415); ; Estimated MW of expressed protein : 163096 Daltons