cDNA Clone FGD6

• Plasmid: pGFP-TAP-BN FGD6
• Parent Plasmid: pGFP TAP BN
• Expressed: GFP-TAP FGD6
• Genbank: NM_018351
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

gcggccgcgatgacttctgcagccgagataaagaagccaccagtggcccccaagcccaagtttgttgtggcaaataacaagccagccccacctcctattgcacctaaacccgacattgtgatttctagtgttccacagtcgacaaagaaaatgaaaccagcaatagccccaaaaccaaaagtcctgaagacctcacctgttcgagagattgggcagtcgccatcaaggaaaatcatgttgaacctggaagggcataaacaggaattagctgaaagcactgacaactttaattgtaaatatgaaggcaatcagagcaatgattatatttcaccaatgtgttcctgcagttctgagtgtatccataagctgggccatagagagaatttgtgtgtaaagcagcttgtcttagagcccctggaaatgaatgaaaatttagaaaacagtaaaattgatgagact
ttgactataaaaactaggagtaaatgtgatttgtatggtgaaaaagccaa
gaaccagggtggagttgttttaaaggcaagcgttttagaagaggagctca
aagatgccttaatacaccaaatgccaccttttatttctgcacagaagcac
aggcccacagacagcccagaaatgaatggtggctgtaattcaaatggaca
attcagaattgaatttgcggatttgtcaccttccccatccagctttgaaa
aagttcctgatcatcacagttgccacttacagcttcctagtgatgaatgt
gaacattttgaaacttgccaggatgacagtgaaaaaagcaataattgctt
tcagtcatctgaactagaggctctggaaaatgggaaaaggagtactttaa
tatcttcagatggagttagtaagaaatcagaagtcaaagaccttggtccc
ttagaaattcatttagtaccatataccccaaaatttccaactcccaagcc
cagaaagacacgaactgctcgtctgttacgccaaaagtgtgtagatactc
ctagtgaaagcactgaagaaccggggaattcagacagtagctcttcctgt
cttactgaaaatagtttgaaaatcaataaaatcagtgttctgcatcagaa
tgttttgtgtaagcaggaacaggtggataaaatgaagctaggaaataaaa
gtgaattgaatatggaatccaacagtgatgcacaggacttagtcaattca
cagaaagccatgtgtaatgaaacaacttcctttgaaaaaatggcaccttc
ttttgataaagactctaatttgagttctgacagcacaactgtagatggtt
ctagtatgtcgcttgctgtggacgaagggaccggttttataagatgtact
gtatctatgagcctgcctaagcagctcaaattaacttgcaatgaacattt
gcaatctgggagaaacctgggagtttctgcccctcaaatgcaaaaggaat
ctgttataaaagaggaaaattctctacgaattgtccccaaaaaacctcaa
agacatagcttgcctgctacaggagtgcttaaaaaggctgcctccgagga
gcttttggaaaaaagttcttatccttcaagtgaagaaaaaagttcagaga
agagtctagaaagaaatcaccttcagcatttgtgtgcccaaaaccgtggt
gtgtcatcctcctttgatatgcctaaacgggcttcagaaaagccagtgtg
gaagttacctcatcctattttacccttttcagggaacccagaattcttaa
agtctgtcaccgtatcgtcaaacagtgagccttcaacagccctaaccaag
cccagagcaaaatcgttatctgctatggatgtggaaaagtgcactaagcc
ttgcaaagactctacaaagaaaaactcttttaaaaagttgctcagcatga
aactgtccatctgtttcatgaagagtgactttcaaaaattttggtccaag
agtagccaacttggagacaccaccacaggccacctctccagtggggagca
gaaggggattgaaagtgattggcaaggcttgttggtaggagaggagaaga
gaagtaaacccatcaaggcatattccacagaaaactatagcctggaatct
caaaagaagaggaagaagtctcggggccagaccagtgcagctaatggtct
gagagctgagtctttggatgaccaaatgctctcccgggagtcatcatctc
aggcaccttacaagtctgttacaagcctctgtgcaccggagtatgaaaat
atacgccattatgaggaaataccagagtacgagaacttgccatttattat
ggctatacggaaaactcaagagttggaatggcagaattccagcagcatgg
aggacgctgatgcaaatgtgtatgaggtagaagagccgtatgaagctcca
gatggccagctgcagcttggacccagacatcagcattccagttcaggagc
atcccaggaggaacagaatgatcttggtcttggtgaccttccctctgatg
aggaggaaatcatcaacagttctgatgaagatgatgtcagctctgagtca
agtaaaggagagcctgacccactggaagataaacaggatgaagataatgg
aatgaaaagtaaagttcatcatattgccaaggagatcatgagctcagaga
aagtgtttgtggatgtgttaaaacttttgcatattgatttccgggatgca
gtagctcatgcttccaggcaacttgggaaaccagtgattgaggaccggat
tctaaatcagatcctatactacttgcctcagctgtatgagctcaaccggg
atctcttgaaggaactggaggaaagaatgttgcactggactgaacaccaa
agaattgctgatatctttgtaaagaagggaccatatctaaaaatgtattc
cacatacatcaaagaatttgataagaatatagccttgctggatgaacagt
gcaagaaaaatccaggttttgctgctgttgttagagaatttgagatgagc
cctcgctgtgctaatctggccctcaagcactacctgctcaagccggttca
gaggatcccccagtacaggctgttgctgacagattatttgaagaatctca
tagaagatgctggagattacagagacactcaagatgcccttgctgttgtt
atagaggtagccaaccacgccaatgacaccatgaagcaaggagacaactt
tcagaaacttatgcaaattcagtacagcttaaatggacaccatgaaattg
tgcagcctggtcgggtttttctcaaagaaggaattctgatgaagctgtct
cggaaagtgatgcaacctcgaatgtttttcctgtttaatgatgccctgct
gtatacaacaccagtgcagtctgggatgtataaactgaacaacatgctct
cactggctggaatgaaggtcagaaaacctacccaagaagcctatcagaat
gaattaaagattgaaagtgtagaacgttccttcattctctcagccagttc
tgccacagaaagggatgaatggctagaagcgatttccagggcaatagaag
agtatgccaagaaaagaatcaccttctgtcctagtaggagtcttgatgag
gcagactcagaaaataaagaagaagttagtcctcttggatcgaaggctcc
catctggattcctgataccagagccacaatgtgtatgatctgcacaagcg
aattcactctcacctggagacgacaccactgccgggcctgtggaaagatt
gtatgccaagcttgttcgtctaataagtatggcttagattacctgaaaaa
tcaaccagcaagagtatgtgaacattgtttccaagaactgcagaaattag
atcaccagcactcccctaggattggatctcctggaaatcacaaatctcct
tcaagtgccttatcatcagtcttacatagcattccatcagggaggaaaca
gaaaaaaatcccagctgctctcaaagaagtatcagcaaacacagaggatt
cttctatgagtggctacttgtacagatcaaagggcaataaaaaaccctgg
aaacacttttggtttgtcataaaaaataaagtactatatacatatgctgc
aagtgaggacgtggccgctttggagagtcagcctttattaggattcactg
ttattcaagttaaagatgagaattccgagtctaaagtatttcagttactg
cacaaaaacatgttattttatgtattcaaagcagaggatgctcactcggc
tcagaagtggatagaagcatttcaggaaggcacaatattgtaggcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSRSG
TAAAMTSAAEIKKPPVAPKPKFVVANNKPAPPPIAPKPDIVISSVPQSTK
KMKPAIAPKPKVLKTSPVREIGQSPSRKIMLNLEGHKQELAESTDNFNCK
YEGNQSNDYISPMCSCSSECIHKLGHRENLCVKQLVLEPLEMNENLENSK
IDETLTIKTRSKCDLYGEKAKNQGGVVLKASVLEEELKDALIHQMPPFIS
AQKHRPTDSPEMNGGCNSNGQFRIEFADLSPSPSSFEKVPDHHSCHLQLP
SDECEHFETCQDDSEKSNNCFQSSELEALENGKRSTLISSDGVSKKSEVK
DLGPLEIHLVPYTPKFPTPKPRKTRTARLLRQKCVDTPSESTEEPGNSDS
SSSCLTENSLKINKISVLHQNVLCKQEQVDKMKLGNKSELNMESNSDAQD
LVNSQKAMCNETTSFEKMAPSFDKDSNLSSDSTTVDGSSMSLAVDEGTGF
IRCTVSMSLPKQLKLTCNEHLQSGRNLGVSAPQMQKESVIKEENSLRIVP
KKPQRHSLPATGVLKKAASEELLEKSSYPSSEEKSSEKSLERNHLQHLCA
QNRGVSSSFDMPKRASEKPVWKLPHPILPFSGNPEFLKSVTVSSNSEPST
ALTKPRAKSLSAMDVEKCTKPCKDSTKKNSFKKLLSMKLSICFMKSDFQK
FWSKSSQLGDTTTGHLSSGEQKGIESDWQGLLVGEEKRSKPIKAYSTENY
SLESQKKRKKSRGQTSAANGLRAESLDDQMLSRESSSQAPYKSVTSLCAP
EYENIRHYEEIPEYENLPFIMAIRKTQELEWQNSSSMEDADANVYEVEEP
YEAPDGQLQLGPRHQHSSSGASQEEQNDLGLGDLPSDEEEIINSSDEDDV
SSESSKGEPDPLEDKQDEDNGMKSKVHHIAKEIMSSEKVFVDVLKLLHID
FRDAVAHASRQLGKPVIEDRILNQILYYLPQLYELNRDLLKELEERMLHW
TEHQRIADIFVKKGPYLKMYSTYIKEFDKNIALLDEQCKKNPGFAAVVRE
FEMSPRCANLALKHYLLKPVQRIPQYRLLLTDYLKNLIEDAGDYRDTQDA
LAVVIEVANHANDTMKQGDNFQKLMQIQYSLNGHHEIVQPGRVFLKEGIL
MKLSRKVMQPRMFFLFNDALLYTTPVQSGMYKLNNMLSLAGMKVRKPTQE
AYQNELKIESVERSFILSASSATERDEWLEAISRAIEEYAKKRITFCPSR
SLDEADSENKEEVSPLGSKAPIWIPDTRATMCMICTSEFTLTWRRHHCRA
CGKIVCQACSSNKYGLDYLKNQPARVCEHCFQELQKLDHQHSPRIGSPGN
HKSPSSALSSVLHSIPSGRKQKKIPAALKEVSANTEDSSMSGYLYRSKGN
KKPWKHFWFVIKNKVLYTYAASEDVAALESQPLLGFTVIQVKDENSESKV
FQLLHKNMLFYVFKAEDAHSAQKWIEAFQEGTIL*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU5603
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. sequence differences (all silent) compared with NM_018351:; t69c(N23), t396c(V132), g513a(G171), c1962t(L654), a2388g(P796), t2847c(H949), g2850a(Q950), t4245c(H1415)