cDNA Clone 53BP1

• Synonyms: TP53BP1
• Plasmid: pCMV5-HA 53BP1 2-1691-PDGFB 528-1106(end)
• Parent Plasmid: pCMV5
• Expressed: HA-53BP1 2-1691-PDGFB 528-1106(end)
• Genbank: NM_005657 for 53BP1, NM_002609 for PDGFRB
• Tag: HA
• Species: Human

Sequence of Insert

ggtaccGCCGCCACCATGTACCCATACGACGTACCAGACTATGCCGACCCTACTGGAAGTCAGTTGGATTCAGATTTCTCTCAGCAAGATACTCCTTGCCTGATAATTGAAGATTCTCAGCCTGAAAGCCAGGTTCTAGAGGATGATTCTGGTTCTCACTTCAGTATGCTATCTCGACACCTTCCTAATCTCCAGACGCACAAAGAAAATCCTGTGTTGGATGTTGTGTCCAATCCTGAACAAACAGCTGGAGAAGAACGAGGAGACGGTAATAGTGGGTTCAATGAACATTTGAAAGAAAACAAGGTTGCAGACCCTGTGGATTCTTCTAACTTGGACACATGTGGTTCCATCAGTCAGGTCATTGAGCAGTTACCTCAGCCAAACAGGACAAGCAGTGTTCTGGGAATGTCAGTGGAATCTGCTCCTGCTGTGGAGGAAGAGAAGGGAGAAGAGTTGGAACAGAAGGAGAAAGAGAAGGAAGAAGATACTTCAGGCAATACTACACATTCCCTTGGTGCTGAAGATACTGCCTCATCACAGTTGGGTTTTGGGGTTCTGGAACTCTCCCAGAGCCAGGATGTTGAGGAAAATACTGTGCCATATGAAGTGGACAAAGAGCAGCTACAATCAGTAACCACCAACTCTGGTTATACCAGGCTGTCTGATGTGGATGCTAATACTGCAATTAAGCATGAAGAACAGTCCAACGAAGATATCCCCATAGCAGAACAGTCCAGCAAGGACATCCCTGTGACAGCACAGCCCAGTAAGGATGTACATGTTGTAAAAGAGCAAAATCCACCACCTGCAAGGTCAGAGGACATGCCTTTTAGCCCCAAAGCATCTGTTGCTGCTATGGAAGCAAAAGAACAGTTGTCTGCACAAGAACTTATGGAAAGTGGACTGCAGATTCAGAAGTCACCAGAGCCTGAGGTTTTGTCAACTCAGGAAGACTTGTTTGACCAGAGCAATAAAACAGTATCTTCTGATGGTTGCTCTACTCCTTCAAGGGAGGAAGGTGGGTGTTCTTTGGCTTCCACTCCTGCCACCACTCTGCATCTCCTGCAGCTCTCTGGTCAGAGGTCCCTTGTTCAGGACAGTCTTTCCACGAATTCTTCAGATCTTGTTGCTCCTTCTCCTGATGCTTTCCGATCTACTCCTTTTATCGTTCCTAGCAGTCCCACAGAGCAAGAAGGGAGACAAGATAAGCCAATGGACACGTCAGTGTTATCTGAAGAAGGAGGAGAGCCTTTTCAGAAGAAACTTCAAAGTGGTGAACCAGTGGAGTTAGAAAACCCCCCTCTCCTGCCTGAGTCCACTGTATCACCACAAGCCTCAACACCAATATCTCAGAGCACACCAGTCTTCCCTCCTGGGTCACTTCCTATCCCATCCCAGCCTCAGTTTTCTCATGACATTTTTATTCCTTCCCCAAGTCTGGAAGAACAATCAAATGATGGGAAGAAAGATGGAGATATGCATAGTTCATCTTTGACAGTTGAGTGTTCTAAAACTTCAGAGATTGAACCAAAGAATTCCCCTGAGGATCTTGGGCTATCTTTGACAGGGGATTCTTGCAAGTTGATGCTTTCTACAAGTGAATATAGTCAGTCCCCAAAGATGGAGAGTTTGAGTTCTCACAGAATTGATGAAGATGGAGAAAACACACAGATTGAGGATACGGAGCCCATGTCTCCAGTTCTCAATTCTAAATTTGTTCCTGCTGAAAATGATAGTATCCTGATGAATCCAGCACAGGATGGTGAAGTACAACTGAGTCAGAATGATGACAAAACAAAGGGAGATGATACAGACACCAGGGATGACATTAGTATTTTAGCCACTGGTTGCAAGGGCAGAGAAGAAACGGTAGCAGAAGATGTTTGTATTGATCTCACTTGTGATTCGGGGAGTCAGGCAGTTCCGTCACCAGCTACTCGATCTGAGGCACTTTCTAGTGTGTTAGATCAGGAGGAAGCTATGGAAATTAAAGAACACCATCCAGAGGAGGGGTCTTCAGGGTCTGAGGTGGAAGAAATCCCTGAGACACCTTGTGAAAGTCAAGGAGAGGAACTCAAAGAAGAAAATATGGAGAGTGTTCCGTTGCACCTTTCTCTGACTGAAACTCAGTCCCAAGGG
TTGTGTCTTCAAAAGGAAATGCCAAAAAAAGAATGCTCAGAAGCTATGGA
AGTTGAAACCAGTGTGATTAGTATTGATTCCCCTCAAAAGTTGGCAATAC
TTGACCAAGAATTGGAACATAAGGAACAGGAAGCTTGGGAAGAAGCTACT
TCAGAGGACTCCAGTGTTGTCATTGTAGATGTGAAAGAGCCATCTCCCAG
AGTTGATGTTTCTTGTGAACCTTTGGAGGGAGTGGAGAAGTGCTCAGATT
CCCAGTCATGGGAGGATATTGCTCCAGAAATAGAACCATGTGCTGAGAAT
AGATTAGACACCAAGGAAGAAAAGAGTGTAGAATATGAAGGAGATCTGAA
ATCAGGGACTGCAGAAACAGAACCTGTAGAGCAAGATTCTTCACAGCCTT
CCTTACCTTTAGTGAGAGCAGATGATCCTTTAAGACTTGACCAGGAGTTG
CAGCAGCCCCAAACTCAGGAGAAAACAAGTAATTCATTAACAGAAGACTC
AAAAATGGCTAATGCAAAGCAGCTAAGCTCAGATGCAGAGGCCCAGAAGC
TGGGGAAGCCCTCTGCCCATGCCTCACAAAGCTTCTGTGAAAGTTCTAGT
GAAACCCCATTTCATTTCACTTTGCCTAAAGAAGGTGATATCATCCCACC
ATTGACTGGTGCAACCCCACCTCTTATTGGGCACCTAAAATTGGAGCCCA
AGAGACACAGTACTCCTATTGGTATTAGCAACTATCCAGAAAGCACCATA
GCAACCAGTGATGTCATGTCTGAAAGCATGGTGGAGACCCATGATCCCAT
ACTTGGGAGTGGAAAAGGGGATTCTGGGGCTGCCCCAGACGTGGATGATA
AATTATGTCTAAGAATGAAACTGGTTAGTCCTGAGACTGAGGCGAGTGAA
GAGTCTTTGCAGTTCAACCTGGAAAAGCCTGCAACTGGTGAAAGAAAAAA
TGGATCTACTGCTGTTGCTGAGTCTGTTGCCAGTCCCCAGAAGACCATGT
CTGTGTTGAGCTGTATCTGTGAAGCCAGGCAAGAGAATGAGGCTCGAAGT
GAGGATCCCCCCACCACACCCATCAGGGGGAACTTGCTCCACTTTCCAAG
TTCTCAAGGAGAAGAGGAGAAAGAAAAATTGGAGGGTGACCATACAATCA
GGCAGAGTCAACAGCCTATGAAGCCCATTAGTCCTGTCAAGGACCCTGTT
TCTCCTGCTTCCCAGAAGATGGTCATACAAGGGCCATCCAGTCCTCAAGG
AGAGGCAATGGTGACAGATGTGCTAGAAGACCAGAAAGAAGGACGGAGTA
CTAATAAGGAAAATCCTAGTAAGGCCTTGATTGAAAGGCCCAGCCAAAAT
AACATAGGAATCCAAACCATGGAGTGTTCCTTGAGGGTCCCAGAAACTGT
TTCAGCAGCAACCCAGACTATAAAGAATGTGTGTGAGCAGGGGACCAGTA
CAGTGGACCAGAACTTTGGAAAGCAAGATGCCACAGTTCAGACTGAGAGG
GGGAGTGGTGAGAAACCAGTCAGTGCTCCTGGGGATGATACAGAGTCGCT
CCATAGCCAGGGAGAAGAAGAGTTTGATATGCCTCAGCCTCCACATGGCC
ATGTCTTACATCGTCACATGAGAACAATCCGGGAAGTACGCACACTTGTC
ACTCGTGTCATTACAGATGTGTATTATGTGGATGGAACAGAAGTAGAAAG
AAAAGTAACTGAGGAGACTGAAGAGCCAATTGTAGAGTGTCAGGAGTGTG
AAACTGAAGTTTCCCCTTCACAGACTGGGGGCTCCTCAGGTGACCTGGGG
GATATCAGCTCCTTCTCCTCCAAGGCATCCAGCTTACACCGCACATCAAG
TGGGACAAGTCTCTCAGCTATGCACAGCAGTGGAAGCTCAGGGAAAGGAG
CCGGACCACTCAGAGGGAAAACCAGCGGGACAGAACCCGCAGATTTTGCC
TTACCCAGCTCCCGAGGAGGCCCAGGAAAACTGAGTCCTAGAAAAGGGGT
CAGTCAGACAGGGACGCCAGTGTGTGAGGAGGATGGTGATGCAGGCCTTG
GCATCAGACAGGGAGGGAAGGCTCCAGTCACGCCTCGTGGGCGTGGGCGA
AGGGGCCGCCCACCTTCTCGGACCACTGGAACCAGAGAAACAGCTGTGCC
TGGCCCCTTGGGCATAGAGGACATTTCACCTAACTTGTCACCAGATGATA
AATCCTTCAGCCGTGTCGTGCCCCGAGTGCCAGACTCCACCAGACGAACA
GATGTGGGTGCTGGTGCTTTGCGTCGTAGTGACTCTCCAGAAATTCCTTT
CCAGGCTGCTGCTGGCCCTTCTGATGGCTTAGATGCCTCCTCTCCAGGAA
ATAGCTTTGTAGGGCTCCGTGTTGTAGCCAAGTGGTCATCCAATGGCTAC
TTTTACTCTGGGAAAATCACACGAGATGTCGGAGCTGGGAAGTATAAATT
GCTCTTTGATGATGGGTACGAATGTGATGTGTTGGGCAAAGACATTCTGT
TATGTGACCCCATCCCGCTGGACACTGAAGTGACGGCCCTCTCGGAGGAT
GAGTATTTCAGTGCAGGAGTGGTGAAAGGACATAGGAAGGAGTCTGGGGA
ACTGTACTACAGCATTGAAAAAGAAGGCCAAAGAAAGTGGTATAAGCGAA
TGGCTGTCATCCTGTCCTTGGAGCAAGGAAACAGACTGAGAGAGCAGTAT
GGGCTTGGCCCCTATGAAGCAGTAACACCTCTTACAAAGGCAGCAGATAT
CAGCTTAGACAATTTGGTGGAAGGGAAGCGGAAACGGCGCAGTAACGTCA
GCTCCCCAGCCACCCCTACTGCCTCCAGTAGCAGCAGCACAACCCCTACC
CGAAAGATCACAGAAAGTCCTCGTGCCTCCATGGGAGTTCTCTCAGGCAA
AAGAAAACTTATCACTTCTGAAGAGGAACGGTCCCCTGCCAAGCGAGGTC
GCAAGTCTGCCACAGTAAAACCTGCCTTGCCCTTTAAGGTGGTGGTTATC
TCAGCCATCCTGGCCCTGGTGGTGCTCACCATCATCTCCCTTATCATCCT
CATCATGCTTTGGCAGAAGAAGCCACGTTACGAGATCCGATGGAAGGTGA
TTGAGTCTGTGAGCTCTGACGGCCATGAGTACATCTACGTGGACCCCATG
CAGCTGCCCTATGACTCCACGTGGGAGCTGCCGCGGGACCAGCTTGTGCT
GGGACGCACCCTCGGCTCTGGGGCCTTTGGGCAGGTGGTGGAGGCCACGG
CTCATGGCCTGAGCCATTCTCAGGCCACGATGAAAGTGGCCGTCAAGATG
CTTAAATCCACAGCCCGCAGCAGTGAGAAGCAAGCCCTTATGTCGGAGCT
GAAGATCATGAGTCACCTTGGGCCCCACCTGAACGTGGTCAACCTGTTGG
GGGCCTGCACCAAAGGAGGACCCATCTATATCATCACTGAGTACTGCCGC
TACGGAGACCTGGTGGACTACCTGCACCGCAACAAACACACCTTCCTGCA
GCACCACTCCGACAAGCGCCGCCCGCCCAGCGCGGAGCTCTACAGCAATG
CTCTGCCCGTTGGGCTCCCCCTGCCCAGCCATGTGTCCTTGACCGGGGAG
AGCGACGGTGGCTACATGGACATGAGCAAGGACGAGTCGGTGGACTATGT
GCCCATGCTGGACATGAAAGGAGACGTCAAATATGCAGACATCGAGTCCT
CCAACTACATGGCCCCTTACGATAACTACGTTCCCTCTGCCCCTGAGAGG
ACCTGCCGAGCAACTTTGATCAACGAGTCTCCAGTGCTAAGCTACATGGA
CCTCGTGGGCTTCAGCTACCAGGTGGCCAATGGCATGGAGTTTCTGGCCT
CCAAGAACTGCGTCCACAGAGACCTGGCGGCTAGGAACGTGCTCATCTGT
GAAGGCAAGCTGGTCAAGATCTGTGACTTTGGCCTGGCTCGAGACATCAT
GCGGGACTCGAATTACATCTCCAAAGGCAGCACCTTTTTGCCTTTAAAGT
GGATGGCTCCGGAGAGCATCTTCAACAGCCTCTACACCACCCTGAGCGAC
GTGTGGTCCTTCGGGATCCTGCTCTGGGAGATCTTCACCTTGGGTGGCAC
CCCTTACCCAGAGCTGCCCATGAACGAGCAGTTCTACAATGCCATCAAAC
GGGGTTACCGCATGGCCCAGCCTGCCCATGCCTCCGACGAGATCTATGAG
ATCATGCAGAAGTGCTGGGAAGAGAAGTTTGAGATTCGGCCCCCCTTCTC
CCAGCTGGTGCTGCTTCTCGAGAGACTGTTGGGCGAAGGTTACAAAAAGA
AGTACCAGCAGGTGGATGAGGAGTTTCTGAGGAGTGACCACCCAGCCATC
CTTCGGTCCCAGGCCCGCTTGCCTGGGTTCCATGGCCTCCGATCTCCCCT
GGACACCAGCTCCGTCCTCTATACTGCCGTGCAGCCCAATGAGGGTGACA
ACGACTATATCATCCCCCTGCCTGACCCCAAACCCGAGGTTGCTGACGAG
GGCCCACTGGAGGGTTCCCCCAGCCTAGCCAGCTCCACCCTGAATGAAGT
CAACACCTCCTCAACCATCTCCTGTGACAGCCCCCTGGAGCCCCAGGACG
AACCAGAGCCAGAGCCCCAGCTTGAGCTCCAGGTGGAGCCGGAGCCAGAG
CTGGAACAGTTGCCGGATTCGGGGTGCCCTGCGCCTCGGGCGGAAGCAGA
GGATAGCTTCCTGTAGAACcctgcagg

Amino Acid Sequence

MYPYDVPDYADPTGSQLDSDFSQQDTPCLIIEDSQPESQVLEDDSGSHFS
MLSRHLPNLQTHKENPVLDVVSNPEQTAGEERGDGNSGFNEHLKENKVAD
PVDSSNLDTCGSISQVIEQLPQPNRTSSVLGMSVESAPAVEEEKGEELEQ
KEKEKEEDTSGNTTHSLGAEDTASSQLGFGVLELSQSQDVEENTVPYEVD
KEQLQSVTTNSGYTRLSDVDANTAIKHEEQSNEDIPIAEQSSKDIPVTAQ
PSKDVHVVKEQNPPPARSEDMPFSPKASVAAMEAKEQLSAQELMESGLQI
QKSPEPEVLSTQEDLFDQSNKTVSSDGCSTPSREEGGCSLASTPATTLHL
LQLSGQRSLVQDSLSTNSSDLVAPSPDAFRSTPFIVPSSPTEQEGRQDKP
MDTSVLSEEGGEPFQKKLQSGEPVELENPPLLPESTVSPQASTPISQSTP
VFPPGSLPIPSQPQFSHDIFIPSPSLEEQSNDGKKDGDMHSSSLTVECSK
TSEIEPKNSPEDLGLSLTGDSCKLMLSTSEYSQSPKMESLSSHRIDEDGE
NTQIEDTEPMSPVLNSKFVPAENDSILMNPAQDGEVQLSQNDDKTKGDDT
DTRDDISILATGCKGREETVAEDVCIDLTCDSGSQAVPSPATRSEALSSV
LDQEEAMEIKEHHPEEGSSGSEVEEIPETPCESQGEELKEENMESVPLHL
SLTETQSQGLCLQKEMPKKECSEAMEVETSVISIDSPQKLAILDQELEHK
EQEAWEEATSEDSSVVIVDVKEPSPRVDVSCEPLEGVEKCSDSQSWEDIA
PEIEPCAENRLDTKEEKSVEYEGDLKSGTAETEPVEQDSSQPSLPLVRAD
DPLRLDQELQQPQTQEKTSNSLTEDSKMANAKQLSSDAEAQKLGKPSAHA
SQSFCESSSETPFHFTLPKEGDIIPPLTGATPPLIGHLKLEPKRHSTPIG
ISNYPESTIATSDVMSESMVETHDPILGSGKGDSGAAPDVDDKLCLRMKL
VSPETEASEESLQFNLEKPATGERKNGSTAVAESVASPQKTMSVLSCICE
ARQENEARSEDPPTTPIRGNLLHFPSSQGEEEKEKLEGDHTIRQSQQPMK
PISPVKDPVSPASQKMVIQGPSSPQGEAMVTDVLEDQKEGRSTNKENPSK
ALIERPSQNNIGIQTMECSLRVPETVSAATQTIKNVCEQGTSTVDQNFGK
QDATVQTERGSGEKPVSAPGDDTESLHSQGEEEFDMPQPPHGHVLHRHMR
TIREVRTLVTRVITDVYYVDGTEVERKVTEETEEPIVECQECETEVSPSQ
TGGSSGDLGDISSFSSKASSLHRTSSGTSLSAMHSSGSSGKGAGPLRGKT
SGTEPADFALPSSRGGPGKLSPRKGVSQTGTPVCEEDGDAGLGIRQGGKA
PVTPRGRGRRGRPPSRTTGTRETAVPGPLGIEDISPNLSPDDKSFSRVVP
RVPDSTRRTDVGAGALRRSDSPEIPFQAAAGPSDGLDASSPGNSFVGLRV
VAKWSSNGYFYSGKITRDVGAGKYKLLFDDGYECDVLGKDILLCDPIPLD
TEVTALSEDEYFSAGVVKGHRKESGELYYSIEKEGQRKWYKRMAVILSLE
QGNRLREQYGLGPYEAVTPLTKAADISLDNLVEGKRKRRSNVSSPATPTA
SSSSSTTPTRKITESPRASMGVLSGKRKLITSEEERSPAKRGRKSATVKP
ALPFKVVVISAILALVVLTIISLIILIMLWQKKPRYEIRWKVIESVSSDG
HEYIYVDPMQLPYDSTWELPRDQLVLGRTLGSGAFGQVVEATAHGLSHSQ
ATMKVAVKMLKSTARSSEKQALMSELKIMSHLGPHLNVVNLLGACTKGGP
IYIITEYCRYGDLVDYLHRNKHTFLQHHSDKRRPPSAELYSNALPVGLPL
PSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYD
NYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRD
LAARNVLICEGKLVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIF
NSLYTTLSDVWSFGILLWEIFTLGGTPYPELPMNEQFYNAIKRGYRMAQP
AHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLERLLGEGYKKKYQQVDEE
FLRSDHPAILRSQARLPGFHGLRSPLDTSSVLYTAVQPNEGDNDYIIPLP
DPKPEVADEGPLEGSPSLASSTLNEVNTSSTISCDSPLEPQDEPEPEPQL
ELQVEPEPELEQLPDSGCPAPRAEAEDSFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5506
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences in 53BP1 compared with NM_005657 CDS:; c1590t (silent S530); a1647g (silent E549); ; Estimated MW of expressed protein : 248182 Daltons