cDNA Clone SLX4 (yeast)

• Plasmid: pYES2-yeast SLX4.FLAG.6His
• Parent Plasmid: pYES2
• Genbank: Z73307
• Tag: FLAG
• Species: Yeast

Sequence of Insert

ggatccCTAACCATGGAACTTCAGAGGGCACAACGGAATTTGAAGTTTTTACAAAACGAGGATTATGTGAACGTTACCGATCAAACTAACCTGAATGGAGAGAGCCAGAATGCTTACTCTTTAGGAATGGAAACTCAAGTACCGGAAATGCAGTTTTCCTTATCATCTGATGATGATTCAATAGGTACGCAAGTAAAAAGCGTAACTGCACAAAAATCGCCTATGACGCAAGAGACTACGAAAAACGATACAGAAAGAAATAAAGATGTTGATAAAAGTTGTAACCCAGTCAGCACTTCCCATCCCGATTTAGGCGGATCAAATATCGAGGAGAACATATTCATTAACACTCAAATTCAAAGCCGACTTGATGACGCAGAGGAAGAAACAAATCTTAAGTTAAAACTTGAAAAATTCAAGTATTCGTTCAAGAGTAGTAACGCAGATGATACCCACAGCAATGCAAATGTAACAGCTAAAAGAAGACCAGCAATAAGGAAGGCTAATTCGAAACTCAAAACAAAACCTAAAACTAAACGAGACCCTAAAATTATCAAAAATATAACTGATTTCAATATTAATAACTACGAACGTTCAAGGACTGCCAGCTTA
TTAAAGCAATTATCAGGTAAGCACAAGAAGGTTTTGGACATAATAAAGAC
GCAAAATGAAGGAAACAGTGATAAACCTCCCAGAGCAAGGAATAACAAGG
GTGAAAAAGCTACTTTTGATACATATAGTGAACAGGAGTGGAAAGATATT
ATGAAACTTCTTCTACAAAAGTTTCCGCAAAGTGAGGAAACGGATTTGAA
TGAAGTCCAGAAATTTTTATATGGAAGCGAAAAAAGCTCGAATTCTCTAG
ACAATCAAGAATCCTCACAACAAAGACTTTGGACAGCATCACAATTACCA
CCCGAGCTTCCCGATGAGGCTATTCAACCAGAGCAGGAAGAAAGGATACG
CGATACCCAAAGTGCAGTAAATTTCTTATCGTTGTCTCAAGTAATGGATG
ACAAAAGTGAAATTATGAAAGACGAAGAAAGCATAATTATTTCACGGGGA
GATTCTACAAGCTCTCAAGAATATGGAAACGGGTTAGAGCCACAACAACC
GGTAGGAAATGTTGTTGGTGAAGATATTGAACTGGCTGTGGGAACACGTA
TAAATGCATTTTCACTGACAGATTATAAAGCTTGCAAACCTATGTCTGTT
GAGGTAAGTAGAAGATGCGAAAACAGTACGGATAATGATTATGACAATAT
TTCTATTGTCTCAGATACTACAGATGAGACATCCACATTGTTTCCACTAG
ACCAGTATCGGTATGTTTTCATTGAAAATGATGAAAGACCACCATTAGCC
ACCGACACGATAGGCAGCACACAGTTTTTCACTCCAAACACATCGCCTTT
GGATGGGATAATCGATTTAACACAGGAATCATTCAAAGCTGTTCGATCGT
TAATATCACCTTTGAAAGTAGAAAACAACAAAACAGGGGTAACATCACAA
GCATCAAATCAGGTTCAGGTACCAGCTACAAGAACCCCTACAATTATTCC
TCAAAAGAACCTTACTACAACTTTAAAAACTGAAGAAGAAAAGAATAACA
TAGGAAGTTCCATCAGAGTTAAATTATTGCAAGAAAGCGTAGTTAAGCTA
AACCCCAAACTGGTGAAACATAACTTTTATAGAGTTGAGGCAAATGATTC
TGAAGAAGAGGAGACAGAATTTGATGACCAATTCTGCATTGCTGATATCC
AATTGGTAGATTCCTCCAAAATTTCGACTAAAGATTCTACACAAAATCCT
ACTACTAGCAACGACATTATTGATACCTCTGCAGCTAGTTCCATAGCTTC
TCCCGAGAAATTTTGTGAGATCATGATGTCCCAATCAATGAAAGAGCTTC
GACAAAGCTTGAAAACAGTGGGGTTGAAACCTATGAGGACGAAGGTTGAA
ATCATTCAATCCTTACAAACTGCATCTCAAATTCTGTCCACAGCTAATCC
AGATAATAAAGGTGAGCATGGTGGGGTGGCAAATTTCAGTAAGATAGAAA
TATTTGATCATTTAACCGAACTAATTGAAGCCTTCCCTGATTTTTTGGAG
CGAATATATACCTTTGAACCCATTCCGCTAAATGAGCTAATTGAAAAGCT
GTTCAGCGCCGAGCCATTTGTTTCGCAAATCGACGAAATGACCATCAGAG
AATGGGCCGATGTTCAAGGAATATGTCTGAGAAACGATAAAAAGGACTACAAGGACGACGATGACAAGCACCATCACCATCACCATTAGgcggccgc

Amino Acid Sequence

MELQRAQRNLKFLQNEDYVNVTDQTNLNGESQNAYSLGMETQVPEMQFSL
SSDDDSIGTQVKSVTAQKSPMTQETTKNDTERNKDVDKSCNPVSTSHPDL
GGSNIEENIFINTQIQSRLDDAEEETNLKLKLEKFKYSFKSSNADDTHSN
ANVTAKRRPAIRKANSKLKTKPKTKRDPKIIKNITDFNINNYERSRTASL
LKQLSGKHKKVLDIIKTQNEGNSDKPPRARNNKGEKATFDTYSEQEWKDI
MKLLLQKFPQSEETDLNEVQKFLYGSEKSSNSLDNQESSQQRLWTASQLP
PELPDEAIQPEQEERIRDTQSAVNFLSLSQVMDDKSEIMKDEESIIISRG
DSTSSQEYGNGLEPQQPVGNVVGEDIELAVGTRINAFSLTDYKACKPMSV
EVSRRCENSTDNDYDNISIVSDTTDETSTLFPLDQYRYVFIENDERPPLA
TDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLISPLKVENNKTGVTSQ
ASNQVQVPATRTPTIIPQKNLTTTLKTEEEKNNIGSSIRVKLLQESVVKL
NPKLVKHNFYRVEANDSEEEETEFDDQFCIADIQLVDSSKISTKDSTQNP
TTSNDIIDTSAASSIASPEKFCEIMMSQSMKELRQSLKTVGLKPMRTKVE
IIQSLQTASQILSTANPDNKGEHGGVANFSKIEIFDHLTELIEAFPDFLE
RIYTFEPIPLNELIEKLFSAEPFVSQIDEMTIREWADVQGICLRNDKKDY
KDDDDKHHHHHH*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU5454
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression construct for expression of SLX4 with C-terminal FLAG and 6His tags.; ; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.