cDNA Clone SLX4 (yeast)

• Plasmid: pYES2-FLAG-6His-SLX4 (yeast)
• Parent Plasmid: pYES2
• Genbank: Z73307
• Tag: FLAG
• Species: Yeast

Sequence of Insert

ggatccCTAACCATGGACTACAAGGACGACGATGACAAGCACCATCACCATCACCATGAACTTCAGAGGGCACAACGGAATTTGAAGTTTTTACAAAACGAGGATTATGTGAACGTTACCGATCAAACTAACCTGAATGGAGAGAGCCAGAATGCTTACTCTTTAGGAATGGAAACTCAAGTACCGGAAATGCAGTTTTCCTTA
TCATCTGATGATGATTCAATAGGTACGCAAGTAAAAAGCGTAACTGCACA
AAAATCGCCTATGACGCAAGAGACTACGAAAAACGATACAGAAAGAAATA
AAGATGTTGATAAAAGTTGTAACCCAGTCAGCACTTCCCATCCCGATTTA
GGCGGATCAAATATCGAGGAGAACATATTCATTAACACTCAAATTCAAAG
CCGACTTGATGACGCAGAGGAAGAAACAAATCTTAAGTTAAAACTTGAAA
AATTCAAGTATTCGTTCAAGAGTAGTAACGCAGATGATACCCACAGCAAT
GCAAATGTAACAGCTAAAAGAAGACCAGCAATAAGGAAGGCTAATTCGAA
ACTCAAAACAAAACCTAAAACTAAACGAGACCCTAAAATTATCAAAAATA
TAACTGATTTCAATATTAATAACTACGAACGTTCAAGGACTGCCAGCTTA
TTAAAGCAATTATCAGGTAAGCACAAGAAGGTTTTGGACATAATAAAGAC
GCAAAATGAAGGAAACAGTGATAAACCTCCCAGAGCAAGGAATAACAAGG
GTGAAAAAGCTACTTTTGATACATATAGTGAACAGGAGTGGAAAGATATT
ATGAAACTTCTTCTACAAAAGTTTCCGCAAAGTGAGGAAACGGATTTGAA
TGAAGTCCAGAAATTTTTATATGGAAGCGAAAAAAGCTCGAATTCTCTAG
ACAATCAAGAATCCTCACAACAAAGACTTTGGACAGCATCACAATTACCA
CCCGAGCTTCCCGATGAGGCTATTCAACCAGAGCAGGAAGAAAGGATACG
CGATACCCAAAGTGCAGTAAATTTCTTATCGTTGTCTCAAGTAATGGATG
ACAAAAGTGAAATTATGAAAGACGAAGAAAGCATAATTATTTCACGGGGA
GATTCTACAAGCTCTCAAGAATATGGAAACGGGTTAGAGCCACAACAACC
GGTAGGAAATGTTGTTGGTGAAGATATTGAACTGGCTGTGGGAACACGTA
TAAATGCATTTTCACTGACAGATTATAAAGCTTGCAAACCTATGTCTGTT
GAGGTAAGTAGAAGATGCGAAAACAGTACGGATAATGATTATGACAATAT
TTCTATTGTCTCAGATACTACAGATGAGACATCCACATTGTTTCCACTAG
ACCAGTATCGGTATGTTTTCATTGAAAATGATGAAAGACCACCATTAGCC
ACCGACACGATAGGCAGCACACAGTTTTTCACTCCAAACACATCGCCTTT
GGATGGGATAATCGATTTAACACAGGAATCATTCAAAGCTGTTCGATCGT
TAATATCACCTTTGAAAGTAGAAAACAACAAAACAGGGGTAACATCACAA
GCATCAAATCAGGTTCAGGTACCAGCTACAAGAACCCCTACAATTATTCC
TCAAAAGAACCTTACTACAACTTTAAAAACTGAAGAAGAAAAGAATAACA
TAGGAAGTTCCATCAGAGTTAAATTATTGCAAGAAAGCGTAGTTAAGCTA
AACCCCAAACTGGTGAAACATAACTTTTATAGAGTTGAGGCAAATGATTC
TGAAGAAGAGGAGACAGAATTTGATGACCAATTCTGCATTGCTGATATCC
AATTGGTAGATTCCTCCAAAATTTCGACTAAAGATTCTACACAAAATCCT
ACTACTAGCAACGACATTATTGATACCTCTGCAGCTAGTTCCATAGCTTC
TCCCGAGAAATTTTGTGAGATCATGATGTCCCAATCAATGAAAGAGCTTC
GACAAAGCTTGAAAACAGTGGGGTTGAAACCTATGAGGACGAAGGTTGAA
ATCATTCAATCCTTACAAACTGCATCTCAAATTCTGTCCACAGCTAATCC
AGATAATAAAGGTGAGCATGGTGGGGTGGCAAATTTCAGTAAGATAGAAA
TATTTGATCATTTAACCGAACTAATTGAAGCCTTCCCTGATTTTTTGGAG
CGAATATATACCTTTGAACCCATTCCGCTAAATGAGCTAATTGAAAAGCT
GTTCAGCGCCGAGCCATTTGTTTCGCAAATCGACGAAATGACCATCAGAG
AATGGGCCGATGTTCAAGGAATATGTCTGAGAAACGATAAAAAGTAGgcggccgc

Amino Acid Sequence

MDYKDDDDKHHHHHHELQRAQRNLKFLQNEDYVNVTDQTNLNGESQNAYS
LGMETQVPEMQFSLSSDDDSIGTQVKSVTAQKSPMTQETTKNDTERNKDV
DKSCNPVSTSHPDLGGSNIEENIFINTQIQSRLDDAEEETNLKLKLEKFK
YSFKSSNADDTHSNANVTAKRRPAIRKANSKLKTKPKTKRDPKIIKNITD
FNINNYERSRTASLLKQLSGKHKKVLDIIKTQNEGNSDKPPRARNNKGEK
ATFDTYSEQEWKDIMKLLLQKFPQSEETDLNEVQKFLYGSEKSSNSLDNQ
ESSQQRLWTASQLPPELPDEAIQPEQEERIRDTQSAVNFLSLSQVMDDKS
EIMKDEESIIISRGDSTSSQEYGNGLEPQQPVGNVVGEDIELAVGTRINA
FSLTDYKACKPMSVEVSRRCENSTDNDYDNISIVSDTTDETSTLFPLDQY
RYVFIENDERPPLATDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLIS
PLKVENNKTGVTSQASNQVQVPATRTPTIIPQKNLTTTLKTEEEKNNIGS
SIRVKLLQESVVKLNPKLVKHNFYRVEANDSEEEETEFDDQFCIADIQLV
DSSKISTKDSTQNPTTSNDIIDTSAASSIASPEKFCEIMMSQSMKELRQS
LKTVGLKPMRTKVEIIQSLQTASQILSTANPDNKGEHGGVANFSKIEIFD
HLTELIEAFPDFLERIYTFEPIPLNELIEKLFSAEPFVSQIDEMTIREWA
DVQGICLRNDKK*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU5448
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression construct for expression of SLX4 with N-terminal FLAG and 6His tags; ; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.