cDNA Clone 53BP1

• Synonyms: TP53BP1
• Plasmid: pCMV5 53BP1.HA
• Parent Plasmid: pCMV5
• Expressed: 53BP1-HA
• Genbank: NM_005657
• Tag: HA
• Species: Human

Sequence of Insert

ggtaccGCCACCATGGACCCTACTGGAAGTCAGTTGGATTCAGATTTCTCTCAGCAAGATACTCCTTGCCTGATAATTGAAGATTCTCAGCCTGAAAGCCAGGTTCTAGAGGATGATTCTGGTTCTCACTTCAGTATGCTATCTCGACACCTTCCTAATCTCCAGACGCACAAAGAAAATCCTGTGTTGGATGTTGTGTCCAATCCTGAACAAACAGCTGGAGAAGAACGAGGAGACGGTAATAGTGGGTTCAATGAACATTTGAAAGAAAACAAGGTTGCAGACCCTGTGGATTCTTCTAACTTGGACACATGTGGTTCCATCAGTCAGGTCATTGAGCAGTTACCTCAGCCAAACAGGACAAGCAGTGTTCTGGGAATGTCAGTGGAATCTGCTCCTGCTGTGGAGGAAGAGAAGGGAGAAGAGTTGGAACAGAAGGAGAAAGAGAAGGAAGAAGATACTTCAGGCAATACTACACATTCCCTTGGTGCTGAAGATACTGCCTCATCACAGTTGGGTTTTGGGGTTCTGGAACTCTCCCAGAGCCAGGATGTTGAGGAAAATACTGTGCCATATGAAGTGGACAAAGAGCAGCTACAATCAGTAACCACC
AACTCTGGTTATACCAGGCTGTCTGATGTGGATGCTAATACTGCAATTAA
GCATGAAGAACAGTCCAACGAAGATATCCCCATAGCAGAACAGTCCAGCA
AGGACATCCCTGTGACAGCACAGCCCAGTAAGGATGTACATGTTGTAAAA
GAGCAAAATCCACCACCTGCAAGGTCAGAGGACATGCCTTTTAGCCCCAA
AGCATCTGTTGCTGCTATGGAAGCAAAAGAACAGTTGTCTGCACAAGAAC
TTATGGAAAGTGGACTGCAGATTCAGAAGTCACCAGAGCCTGAGGTTTTG
TCAACTCAGGAAGACTTGTTTGACCAGAGCAATAAAACAGTATCTTCTGA
TGGTTGCTCTACTCCTTCAAGGGAGGAAGGTGGGTGTTCTTTGGCTTCCA
CTCCTGCCACCACTCTGCATCTCCTGCAGCTCTCTGGTCAGAGGTCCCTT
GTTCAGGACAGTCTTTCCACGAATTCTTCAGATCTTGTTGCTCCTTCTCC
TGATGCTTTCCGATCTACTCCTTTTATCGTTCCTAGCAGTCCCACAGAGC
AAGAAGGGAGACAAGATAAGCCAATGGACACGTCAGTGTTATCTGAAGAA
GGAGGAGAGCCTTTTCAGAAGAAACTTCAAAGTGGTGAACCAGTGGAGTT
AGAAAACCCCCCTCTCCTGCCTGAGTCCACTGTATCACCACAAGCCTCAA
CACCAATATCTCAGAGCACACCAGTCTTCCCTCCTGGGTCACTTCCTATC
CCATCCCAGCCTCAGTTTTCTCATGACATTTTTATTCCTTCCCCAAGTCT
GGAAGAACAATCAAATGATGGGAAGAAAGATGGAGATATGCATAGTTCAT
CTTTGACAGTTGAGTGTTCTAAAACTTCAGAGATTGAACCAAAGAATTCC
CCTGAGGATCTTGGGCTATCTTTGACAGGGGATTCTTGCAAGTTGATGCT
TTCTACAAGTGAATATAGTCAGTCCCCAAAGATGGAGAGCTTGAGTTCTC
ACAGAATTGATGAAGATGGAGAAAACACACAGATTGAGGATACGGAACCC
ATGTCTCCAGTTCTCAATTCTAAATTTGTTCCTGCTGAAAATGATAGTAT
CCTGATGAATCCAGCACAGGATGGTGAAGTACAACTGAGTCAGAATGATG
ACAAAACAAAGGGAGATGATACAGACACCAGGGATGACATTAGTATTTTA
GCCACTGGTTGCAAGGGCAGAGAAGAAACGGTAGCAGAAGATGTTTGTAT
TGATCTCACTTGTGATTCGGGGAGTCAGGCAGTTCCGTCACCAGCTACTC
GATCTGAGGCACTTTCTAGTGTGTTAGATCAGGAGGAAGCTATGGAAATT
AAAGAACACCATCCAGAGGAGGGGTCTTCAGGGTCTGAGGTGGAAGAAAT
CCCTGAGACACCTTGTGAAAGTCAAGGAGAGGAACTCAAAGAAGAAAATA
TGGAGAGTGTTCCGTTGCACCTTTCTCTGACTGAAACTCAGTCCCAAGGG
TTGTGTCTTCAAAAGGAAATGCCAAAAAAAGAATGCTCAGAAGCTATGGA
AGTTGAAACCAGTGTGATTAGTATTGATTCCCCTCAAAAGTTGGCAATAC
TTGACCAAGAATTGGAACATAAGGAACAGGAAGCTTGGGAAGAAGCTACT
TCAGAGGACTCCAGTGTTGTCATTGTAGATGTGAAAGAGCCATCTCCCAG
AGTTGATGTTTCTTGTGAACCTTTGGAGGGAGTGGAGAAGTGCTCAGATT
CCCAGTCATGGGAGGATATTGCTCCAGAAATAGAACCATGTGCTGAGAAT
AGATTAGACACCAAGGAAGAAAAGAGTGTAGAATATGAAGGAGATCTGAA
ATCAGGGACTGCAGAAACAGAACCTGTAGAGCAAGATTCTTCACAGCCTT
CCTTACCTTTAGTGAGAGCAGATGATCCTTTAAGACTTGACCAGGAGTTG
CAGCAGCCCCAAACTCAGGAGAAAACAAGTAATTCATTAACAGAAGACTC
AAAAATGGCTAATGCAAAGCAGCTAAGCTCAGATGCAGAGGCCCAGAAGC
TGGGGAAGCCCTCTGCCCATGCCTCACAAAGCTTCTGTGAAAGTTCTAGT
GAAACCCCATTTCATTTCACTTTGCCTAAAGAAGGTGATATCATCCCACC
ATTGACTGGTGCAACCCCACCTCTTATTGGGCACCTAAAATTGGAGCCCA
AGAGACACAGTACTCCTATTGGTATTAGCAACTATCCAGAAAGCACCATA
GCAACCAGTGATGTCATGTCTGAAAGCATGGTGGAGACCCATGATCCCAT
ACTTGGGAGTGGAAAAGGGGATTCTGGGGCTGCCCCAGACGTGGATGATA
AATTATGTCTAAGAATGAAACTGGTTAGTCCTGAGACTGAGGCGAGTGAA
GAGTCTTTGCAGTTCAACCTGGAAAAGCCTGCAACTGGTGAAAGAAAAAA
TGGATCTACTGCTGTTGCTGAGTCTGTTGCCAGTCCCCAGAAGACCATGT
CTGTGTTGAGCTGTATCTGTGAAGCCAGGCAAGAGAATGAGGCTCGAAGT
GAGGATCCCCCCACCACACCCATCAGGGGGAACTTGCTCCACTTTCCAAG
TTCTCAAGGAGAAGAGGAGAAAGAAAAATTGGAGGGTGACCATACAATCA
GGCAGAGTCAACAGCCTATGAAGCCCATTAGTCCTGTCAAGGACCCTGTT
TCTCCTGCTTCCCAGAAGATGGTCATACAAGGGCCATCCAGTCCTCAAGG
AGAGGCAATGGTGACAGATGTGCTAGAAGACCAGAAAGAAGGACGGAGTA
CTAATAAGGAAAATCCTAGTAAGGCCTTGATTGAAAGGCCCAGCCAAAAT
AACATAGGAATCCAAACCATGGAGTGTTCCTTGAGGGTCCCAGAAACTGT
TTCAGCAGCAACCCAGACTATAAAGAATGTGTGTGAGCAGGGGACCAGTA
CAGTGGACCAGAACTTTGGAAAGCAAGATGCCACAGTTCAGACTGAGAGG
GGGAGTGGTGAGAAACCAGTCAGTGCTCCTGGGGATGATACAGAGTCGCT
CCATAGCCAGGGAGAAGAAGAGTTTGATATGCCTCAGCCTCCACATGGCC
ATGTCTTACATCGTCACATGAGAACAATCCGGGAAGTACGCACACTTGTC
ACTCGTGTCATTACAGATGTGTATTATGTGGATGGAACAGAAGTAGAAAG
AAAAGTAACTGAGGAGACTGAAGAGCCAATTGTAGAGTGTCAGGAGTGTG
AAACTGAAGTTTCCCCTTCACAGACTGGGGGCTCCTCAGGTGACCTGGGG
GATATCAGCTCCTTCTCCTCCAAGGCATCCAGCTTACACCGCACATCAAG
TGGGACAAGTCTCTCAGCTATGCACAGCAGTGGAAGCTCAGGGAAAGGAG
CCGGACCACTCAGAGGGAAAACCAGCGGGACAGAACCCGCAGATTTTGCC
TTACCCAGCTCCCGAGGAGGCCCAGGAAAACTGAGTCCTAGAAAAGGGGT
CAGTCAGACAGGGACGCCAGTGTGTGAGGAGGATGGTGATGCAGGCCTTG
GCATCAGACAGGGAGGGAAGGCTCCAGTCACGCCTCGTGGGCGTGGGCGA
AGGGGCCGCCCACCTTCTCGGACCACTGGAACCAGAGAAACAGCTGTGCC
TGGCCCCTTGGGCATAGAGGACATTTCACCTAACTTGTCACCAGATGATA
AATCCTTCAGCCGTGTCGTGCCCCGAGTGCCAGACTCCACCAGACGAACA
GATGTGGGTGCTGGTGCTTTGCGTCGTAGTGACTCTCCAGAAATTCCTTT
CCAGGCTGCTGCTGGCCCTTCTGATGGCTTAGATGCCTCCTCTCCAGGAA
ATAGCTTTGTAGGGCTCCGTGTTGTAGCCAAGTGGTCATCCAATGGCTAC
TTTTACTCTGGGAAAATCACACGAGATGTCGGAGCTGGGAAGTATAAATT
GCTCTTTGATGATGGGTACGAATGTGATGTGTTGGGCAAAGACATTCTGT
TATGTGACCCCATCCCGCTGGACACTGAAGTGACGGCCCTCTCGGAGGAT
GAGTATTTCAGTGCAGGAGTGGTGAAAGGACATAGGAAGGAGTCTGGGGA
ACTGTACTACAGCATTGAAAAAGAAGGCCAAAGAAAGTGGTATAAGCGAA
TGGCTGTCATCCTGTCCTTGGAGCAAGGAAACAGACTGAGAGAGCAGTAT
GGGCTTGGCCCCTATGAAGCAGTAACACCTCTTACAAAGGCAGCAGATAT
CAGCTTAGACAATTTGGTGGAAGGGAAGCGGAAACGGCGCAGTAACGTCA
GCTCCCCAGCCACCCCTACTGCCTCCAGTAGCAGCAGCACAACCCCTACC
CGAAAGATCACAGAAAGTCCTCGTGCCTCCATGGGAGTTCTCTCAGGCAA
AAGAAAACTTATCACTTCTGAAGAGGAACGGTCCCCTGCCAAGCGAGGTC
GCAAGTCTGCCACAGTAAAACCTGGTGCAGTAGGGGCAGGAGAGTTTGTG
AGCCCCTGTGAGAGTGGAGACAACACCGGTGAACCCTCTGCCCTGGAAGA
GCAGAGAGGGCCTTTGCCTCTCAACAAGACCTTGTTTCTGGGCTACGCAT
TTCTCCTTACCATGGCCACAACCAGTGACAAGTTGGCCAGCCGCTCCAAA
CTGCCAGATGGTCCTACAGGAAGCAGTGAAGAAGAGGAGGAATTTTTGGA
AATTCCTCCTTTCAACAAGCAGTATACAGAATCCCAGCTTCGAGCAGGAG
CTGGCTATATCCTTGAAGATTTCAATGAAGCCCAGTGTAACACAGCTTAC
CAGTGTCTTCTAATTGCGGATCAGCATTGTCGAACCCGGAAGTACTTCCT
GTGCCTTGCCAGTGGGATTCCTTGTGTGTCTCATGTCTGGGTCCATGATA
GTTGCCATGCCAACCAGCTCCAGAACTACCGTAATTATCTGTTGCCAGCT
GGGTACAGCCTTGAGGAGCAAAGAATTCTGGACTGGCAACCCCGTGAAAA
TCCTTTCCAGAATCTGAAGGTACTCTTGGTATCAGACCAACAGCAGAACT
TCCTGGAGCTCTGGTCTGAGATCCTCATGACTGGTGGTGCAGCCTCTGTG
AAGCAGCACCATTCAAGTGCCCATAACAAAGATATTGCTTTAGGGGTATT
TGATGTGGTGGTGACGGACCCCTCATGCCCAGCCTCGGTGCTGAAGTGTG
CTGAAGCATTGCAGCTGCCTGTGGTGTCACAAGAGTGGGTGATCCAGTGC
CTCATTGTTGGGGAGAGAATTGGATTCAAGCAGCATCCAAAATATAAACA
CGATTATGTTTCTCACTACCCATACGATGTGCCAGATTACGCCTAAgtcgac

Amino Acid Sequence

MDPTGSQLDSDFSQQDTPCLIIEDSQPESQVLEDDSGSHFSMLSRHLPNL
QTHKENPVLDVVSNPEQTAGEERGDGNSGFNEHLKENKVADPVDSSNLDT
CGSISQVIEQLPQPNRTSSVLGMSVESAPAVEEEKGEELEQKEKEKEEDT
SGNTTHSLGAEDTASSQLGFGVLELSQSQDVEENTVPYEVDKEQLQSVTT
NSGYTRLSDVDANTAIKHEEQSNEDIPIAEQSSKDIPVTAQPSKDVHVVK
EQNPPPARSEDMPFSPKASVAAMEAKEQLSAQELMESGLQIQKSPEPEVL
STQEDLFDQSNKTVSSDGCSTPSREEGGCSLASTPATTLHLLQLSGQRSL
VQDSLSTNSSDLVAPSPDAFRSTPFIVPSSPTEQEGRQDKPMDTSVLSEE
GGEPFQKKLQSGEPVELENPPLLPESTVSPQASTPISQSTPVFPPGSLPI
PSQPQFSHDIFIPSPSLEEQSNDGKKDGDMHSSSLTVECSKTSEIEPKNS
PEDLGLSLTGDSCKLMLSTSEYSQSPKMESLSSHRIDEDGENTQIEDTEP
MSPVLNSKFVPAENDSILMNPAQDGEVQLSQNDDKTKGDDTDTRDDISIL
ATGCKGREETVAEDVCIDLTCDSGSQAVPSPATRSEALSSVLDQEEAMEI
KEHHPEEGSSGSEVEEIPETPCESQGEELKEENMESVPLHLSLTETQSQG
LCLQKEMPKKECSEAMEVETSVISIDSPQKLAILDQELEHKEQEAWEEAT
SEDSSVVIVDVKEPSPRVDVSCEPLEGVEKCSDSQSWEDIAPEIEPCAEN
RLDTKEEKSVEYEGDLKSGTAETEPVEQDSSQPSLPLVRADDPLRLDQEL
QQPQTQEKTSNSLTEDSKMANAKQLSSDAEAQKLGKPSAHASQSFCESSS
ETPFHFTLPKEGDIIPPLTGATPPLIGHLKLEPKRHSTPIGISNYPESTI
ATSDVMSESMVETHDPILGSGKGDSGAAPDVDDKLCLRMKLVSPETEASE
ESLQFNLEKPATGERKNGSTAVAESVASPQKTMSVLSCICEARQENEARS
EDPPTTPIRGNLLHFPSSQGEEEKEKLEGDHTIRQSQQPMKPISPVKDPV
SPASQKMVIQGPSSPQGEAMVTDVLEDQKEGRSTNKENPSKALIERPSQN
NIGIQTMECSLRVPETVSAATQTIKNVCEQGTSTVDQNFGKQDATVQTER
GSGEKPVSAPGDDTESLHSQGEEEFDMPQPPHGHVLHRHMRTIREVRTLV
TRVITDVYYVDGTEVERKVTEETEEPIVECQECETEVSPSQTGGSSGDLG
DISSFSSKASSLHRTSSGTSLSAMHSSGSSGKGAGPLRGKTSGTEPADFA
LPSSRGGPGKLSPRKGVSQTGTPVCEEDGDAGLGIRQGGKAPVTPRGRGR
RGRPPSRTTGTRETAVPGPLGIEDISPNLSPDDKSFSRVVPRVPDSTRRT
DVGAGALRRSDSPEIPFQAAAGPSDGLDASSPGNSFVGLRVVAKWSSNGY
FYSGKITRDVGAGKYKLLFDDGYECDVLGKDILLCDPIPLDTEVTALSED
EYFSAGVVKGHRKESGELYYSIEKEGQRKWYKRMAVILSLEQGNRLREQY
GLGPYEAVTPLTKAADISLDNLVEGKRKRRSNVSSPATPTASSSSSTTPT
RKITESPRASMGVLSGKRKLITSEEERSPAKRGRKSATVKPGAVGAGEFV
SPCESGDNTGEPSALEEQRGPLPLNKTLFLGYAFLLTMATTSDKLASRSK
LPDGPTGSSEEEEEFLEIPPFNKQYTESQLRAGAGYILEDFNEAQCNTAY
QCLLIADQHCRTRKYFLCLASGIPCVSHVWVHDSCHANQLQNYRNYLLPA
GYSLEEQRILDWQPRENPFQNLKVLLVSDQQQNFLELWSEILMTGGAASV
KQHHSSAHNKDIALGVFDVVVTDPSCPASVLKCAEALQLPVVSQEWVIQC
LIVGERIGFKQHPKYKHDYVSHYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5387
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 214526 Daltons