cDNA Clone PKD3

• Plasmid: pFastBac.HTc PKD3
• Parent Plasmid: pFastbac HTc
• Expressed: 6His-PKD3
• Genbank: NM_005813
• Tag: 6His
• Species: Human

Sequence of Insert

gtcgacATGTCTGCAAATAATTCCCCTCCATCAGCCCAGAAGTCTGTATTACCCACAGCTATTCCTGCTGTGCTTCCAGCTGCTTCTCCGTGTTCAAGTCCTAAGACGGGACTCTCTGCCCGACTCTCTAATGGAAGCTTCAGTGCACCATCACTCACCAACTCCAGAGGCTCAGTGCATACAGTTTCATTTCTACTGCAAATTGGCCTCACACGGGAGAGTGTTACCATTGAAGCCCAGGAACTGTCTTTATCTGCTGTCAAGGATCTTGTGTGCTCCATAGTTTATCAAAAGTTTCCAGAGTGT
GGATTCTTTGGCATGTATGACAAAATTCTTCTCTTTCGCCATGACATGAA
CTCAGAAAACATTTTGCAGCTGATTACCTCAGCAGATGAAATACATGAAG
GAGACTTAGTGGAAGTGGTTCTTTCAGCTTTAGCCACAGTAGAAGACTTC
CAGATTCGTCCACATACTCTCTATGTACATTCTTACAAAGCTCCTACTTT
CTGTGATTACTGTGGTGAGATGCTCTGGGGATTGGTACGTCAAGGACTGA
AATGTGAAGGCTGTGGATTAAATTACCATAAACGATGTGCCTTCAAGATT
CCAAATAACTGTAGTGGAGTAAGAAAGAGACGTCTGTCAAATGTATCTTT
ACCAGGACCCGGCCTCTCAGTTCCAAGACCCCTACAGCCTGAATATGTAG
CCCTTCCCAGTGAAGAGTCACATGTCCACCAGGAACCAAGTAAGAGAATT
CCTTCTTGGAGTGGTCGCCCAATCTGGATGGAAAAGATGGTAATGTGCAG
AGTGAAAGTTCCACACACATTTGCTGTTCACTCTTACACCCGTCCCACGA
TATGTCAGTACTGCAAGCGGTTACTGAAAGGCCTCTTTCGCCAAGGAATG
CAGTGTAAAGATTGCAAATTCAACTGCCATAAACGCTGTGCATCAAAAGT
ACCAAGAGACTGCCTTGGAGAGGTTACTTTCAATGGAGAACCTTCCAGTC
TGGGAACAGATACAGATATACCAATGGATATTGACAATAATGACATAAAT
AGTGATAGTAGTCGGGGTTTGGATGACACAGAAGAGCCATCACCCCCAGA
AGATAAGATGTTCTTCTTGGATCCATCTGATCTCGATGTGGAAAGAGATG
AAGAAGCCGTTAAAACAATCAGTCCATCAACAAGCAATAATATTCCGCTA
ATGAGGGTTGTACAATCCATCAAGCACACAAAGAGGAAGAGCAGCACAAT
GGTGAAGGAAGGGTGGATGGTCCATTACACCAGCAGGGATAACCTGAGAA
AGAGGCATTATTGGAGACTTGACAGCAAATGTCTAACATTATTTCAGAAT
GAATCTGGATCAAAGTATTATAAGGAAATTCCACTTTCAGAAATTCTCCG
CATATCTTCACCACGAGATTTCACAAACATTTCACAAGGCAGCAATCCAC
ACTGTTTTGAAATCATTACTGATACTATGGTATACTTCGTTGGTGAGAAC
AATGGGGACAGCTCTCATAATCCTGTTCTTGCTGCCACTGGAGTTGGACT
TGATGTAGCACAGAGCTGGGAAAAAGCAATTCGCCAAGCCCTCATGCCTG
TTACTCCTCAAGCAAGTGTTTGCACTTCTCCAGGGCAAGGGAAAGATCAC
AAAGATTTGTCTACAAGTATTTCTGTATCTAATTGTCAGATTCAGGAGAA
TGTGGATATCAGTACTGTTTACCAGATCTTTGCAGATGAGGTGCTTGGTT
CAGGCCAGTTTGGCATCGTTTATGGAGGAAAACATAGAAAGACTGGGAGG
GATGTGGCTATTAAAGTAATTGATAAGATGAGATTCCCCACAAAACAAGA
AAGTCAACTCCGTAATGAAGTGGCTATTTTACAGAATTTGCACCATCCTG
GGATTGTAAACCTGGAATGCATGTTTGAAACCCCAGAACGAGTCTTTGTA
GTAATGGAAAAGCTGCATGGAGATATGTTGGAAATGATTCTATCCAGTGA
GAAAAGTCGGCTTCCAGAACGAATTACTAAATTCATGGTCACACAGATAC
TTGTTGCTTTGAGGAATCTGCATTTTAAGAATATTGTGCACTGTGATTTA
AAGCCAGAAAATGTGCTGCTTGCATCAGCAGAGCCATTTCCTCAGGTGAA
GCTGTGTGACTTTGGATTTGCACGCATCATTGGTGAAAAGTCATTCAGGA
GATCTGTGGTAGGAACTCCAGCATACTTAGCCCCTGAAGTTCTCCGGAGC
AAAGGCTACAACCGTTCCCTAGATATGTGGTCAGTGGGAGTTATCATCTA
TGTGAGCCTCAGTGGCACATTTCCTTTTAATGAGGATGAAGATATAAATG
ACCAAATCCAAAATGCTGCATTTATGTACCCACCAAATCCATGGAGAGAA
ATTTCTGGTGAAGCAATTGATCTGATAAACAATCTGCTTCAAGTGAAGAT
GAGAAAACGTTACAGTGTTGACAAATCTCTTAGTCATCCCTGGCTACAGG
ACTATCAGACTTGGCTTGACCTTAGAGAATTTGAAACTCGCATTGGAGAA
CGTTACATTACACATGAAAGTGATGATGCTCGCTGGGAAATACATGCATA
CACACATAACCTTGTATACCCAAAGCACTTCATTATGGCTCCTAATCCAG
ATGATATGGAAGAAGATCCTTAAactagt

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDMSANNSPPSAQKSV
LPTAIPAVLPAASPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLL
QIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRH
DMNSENILQLITSADEIHEGDLVEVVLSALATVEDFQIRPHTLYVHSYKA
PTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSN
VSLPGPGLSVPRPLQPEYVALPSEESHVHQEPSKRIPSWSGRPIWMEKMV
MCRVKVPHTFAVHSYTRPTICQYCKRLLKGLFRQGMQCKDCKFNCHKRCA
SKVPRDCLGEVTFNGEPSSLGTDTDIPMDIDNNDINSDSSRGLDDTEEPS
PPEDKMFFLDPSDLDVERDEEAVKTISPSTSNNIPLMRVVQSIKHTKRKS
STMVKEGWMVHYTSRDNLRKRHYWRLDSKCLTLFQNESGSKYYKEIPLSE
ILRISSPRDFTNISQGSNPHCFEIITDTMVYFVGENNGDSSHNPVLAATG
VGLDVAQSWEKAIRQALMPVTPQASVCTSPGQGKDHKDLSTSISVSNCQI
QENVDISTVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPT
KQESQLRNEVAILQNLHHPGIVNLECMFETPERVFVVMEKLHGDMLEMIL
SSEKSRLPERITKFMVTQILVALRNLHFKNIVHCDLKPENVLLASAEPFP
QVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRSKGYNRSLDMWSVGV
IIYVSLSGTFPFNEDEDINDQIQNAAFMYPPNPWREISGEAIDLINNLLQ
VKMRKRYSVDKSLSHPWLQDYQTWLDLREFETRIGERYITHESDDARWEI
HAYTHNLVYPKHFIMAPNPDDMEEDP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5386
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; N.B. Sequence differences compared with NM_005813 CDS:; c406t (silent L136); g525c (silent L175); c1671t (silent I557); t1920c (silent C641); t2256c (silent G752); All derive from full length PKD3 clone made by Sharon Matthews.; ; Estimated MW of expressed protein : 104735 Daltons