cDNA Clone MSK1

• Synonyms: RPS6KA5
• Plasmid: pGEX6P-1-MSK1 I382-A802(end).6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-MSK1 I382-A802(end)-6His
• Genbank: AF074393
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATCCTATTCAAGCGTAATGCAGCTGTCATAGACCCTCTTCAGTT
TCACATGGGAGTTGAACGTCCTGGAGTGACAAATGTTGCCAGGAGTGCAA
TGATGAAGGACTCTCCATTCTATCAACACTATGACCTAGATTTGAAGGAC
AAACCCCTGGGAGAAGGTAGTTTTTCAATTTGTCGAAAGTGTGTGCATAA
AAAAAGTAACCAAGCTTTTGCAGTCAAAATAATCAGCAAAAGGATGGAAG
CCAATACTCAAAAGGAAATAACAGCTCTGAAACTCTGTGAAGGACACCCC
AATATTGTGAAGTTGCATGAAGTTTTTCATGATCAGCTTCACACGTTTCT
AGTGATGGAACTTCTGAATGGAGGAGAACTGTTTGAGCGCATTAAGAAAA
AGAAGCACTTCAGTGAGACGGAAGCCAGCTACATCATGAGGAAGCTTGTT
TCAGCTGTAAGCCACATGCATGATGTTGGAGTGGTGCACAGGGATCTGAA
ACCTGAGAATTTATTGTTCACCGATGAAAATGACAATTTGGAAATTAAAA
TAATTGATTTTGGATTTGCACGGCTAAAGCCACCGGATAATCAGCCCCTG
AAGACTCCATGCTTCACCCTTCATTATGCCGCCCCAGAGCTCTTGAATCA
GAACGGCTACGATGAGTCCTGTGACCTGTGGAGCTTGGGCGTCATTTTGT
ACACAATGTTGTCAGGACAGGTTCCCTTCCAATCTCATGACCGAAGTTTG
ACGTGTACCAGCGCGGTGGAAATCATGAAGAAAATTAAAAAGGGAGATTT
CTCCTTTGAAGGAGAAGCCTGGAAGAATGTATCCCAAGAGGCTAAAGATT
TGATCCAAGGACTTCTCACAGTAGATCCAAACAAAAGGCTTAAAATGTCT
GGCTTGAGGTACAATGAATGGCTACAAGATGGAAGTCAGCTGTCCTCCAA
TCCTCTGATGACTCCGGATATTCTAGGATCTTCCGGAGCTGCCGTGCATA
CCTGTGTGAAAGCAACCTTCCACGCCTTTAACAAATACAAGAGAGAGGGG
TTTTGCCTTCAGAATGTTGATAAGGCCCCTTTGGCTAAGAGAAGAAAAAT
GAAAAAGACTAGCACCAGTACCGAGACGCGCAGCAGTTCCAGTGAGAGTT
CCCATTCTTCTTCCTCTCATTCTCACGGTAAAACTACACCCACCAAGACA
CTGCAGCCCAGCAATCCTGCCGACAGCAATAACCCGGAGACCCTCTTCCA
GTTCTCGGACTCAGTAGCTCATCACCATCACCATCACTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFILFKRNAAVIDPLQFH
MGVERPGVTNVARSAMMKDSPFYQHYDLDLKDKPLGEGSFSICRKCVHKK
SNQAFAVKIISKRMEANTQKEITALKLCEGHPNIVKLHEVFHDQLHTFLV
MELLNGGELFERIKKKKHFSETEASYIMRKLVSAVSHMHDVGVVHRDLKP
ENLLFTDENDNLEIKIIDFGFARLKPPDNQPLKTPCFTLHYAAPELLNQN
GYDESCDLWSLGVILYTMLSGQVPFQSHDRSLTCTSAVEIMKKIKKGDFS
FEGEAWKNVSQEAKDLIQGLLTVDPNKRLKMSGLRYNEWLQDGSQLSSNP
LMTPDILGSSGAAVHTCVKATFHAFNKYKREGFCLQNVDKAPLAKRRKMK
KTSTSTETRSSSSESSHSSSSHSHGKTTPTKTLQPSNPADSNNPETLFQF
SDSVAHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5379
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 75318 Daltons