cDNA Clone MSK1

• Synonyms: RPS6KA5
• Plasmid: pGEX6P-1-MSK1 S376D.6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-MSK1 S376D-6His
• Genbank: AF074393
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATGGAGGAGGAGGGTGGCAGCAGCGGCGGCGCCGCGGGGACCAGCGCGGACGGCGGCGACGGAGGAGAGCAGCTCCTCACTGTCAAGCACGAGCTGCGGACTGCTAATTTGACAGGACATGCTGAGAAGGTGGGAATAGAAAATTTTGAGCTCCTGAAGGTCCTAGGAACTGGAGCTTATGGAAAAGTATTTCTAGTTCGTAAAATAAGTGGCCATGATACTGGAAAGCTGTATGCCATGAAAGTTTTGAAAAAGGCAACAATCGTTCAAAAGGCCAAAACCACAGAGCATACAAGGACA
GAACGACAAGTCCTGGAACACATTAGGCAGTCGCCATTTTTGGTAACATT
ACATTATGCTTTCCAGACAGAAACCAAACTTCATCTCATTTTAGATTATA
TAAATGGTGGTGAACTTTTTACTCATCTTTCTCAAAGAGAGCGTTTCACA
GAGCATGAGGTGCAGATTTATGTTGGAGAGATTGTGCTTGCCCTCGAACA
TCTCCACAAGTTGGGGATTATATATCGTGATATTAAGCTTGAGAATATTC
TACTTGATTCTAATGGCCATGTGGTGCTGACAGATTTTGGTCTGAGTAAG
GAGTTTGTGGCTGATGAAACTGAAAGAGCATATTCCTTTTGTGGAACTAT
TGAATACATGGCACCAGATATTGTCAGAGGGGGAGATTCAGGACATGACA
AGGCAGTTGACTGGTGGAGTTTGGGTGTTCTAATGTATGAATTACTAACT
GGAGCATCTCCTTTCACTGTTGATGGAGAAAAAAATTCCCAAGCTGAGAT
ATCTAGGAGAATATTAAAAAGTGAGCCTCCATATCCCCAAGAAATGAGTG
CTTTAGCGAAAGACCTAATTCAGCGTCTTTTGATGAAAGATCCCAAGAAG
AGATTGGGATGTGGTCCACGTGATGCAGATGAAATCAAAGAACATCTCTT
CTTTCAGAAAATAAATTGGGATGATTTAGCCGCCAAAAAAGTGCCTGCAC
CATTTAAGCCAGTCATTCGAGATGAATTAGATGTGAGTAACTTTGCAGAA
GAGTTCACAGAAATGGATCCCACTTATTCTCCCGCAGCCCTGCCCCAGAG
TTCTGAGAAGCTGTTTCAGGGCTATGACTTTGTTGCTCCTTCCATCCTAT
TCAAGCGTAATGCAGCTGTCATAGACCCTCTTCAGTTTCACATGGGAGTT
GAACGTCCTGGAGTGACAAATGTTGCCAGGAGTGCAATGATGAAGGACTC
TCCATTCTATCAACACTATGACCTAGATTTGAAGGACAAACCCCTGGGAG
AAGGTAGTTTTTCAATTTGTCGAAAGTGTGTGCATAAAAAAAGTAACCAA
GCTTTTGCAGTCAAAATAATCAGCAAAAGGATGGAAGCCAATACTCAAAA
GGAAATAACAGCTCTGAAACTCTGTGAAGGACACCCCAATATTGTGAAGT
TGCATGAAGTTTTTCATGATCAGCTTCACACGTTTCTAGTGATGGAACTT
CTGAATGGAGGAGAACTGTTTGAGCGCATTAAGAAAAAGAAGCACTTCAG
TGAGACGGAAGCCAGCTACATCATGAGGAAGCTTGTTTCAGCTGTAAGCC
ACATGCATGATGTTGGAGTGGTGCACAGGGATCTGAAACCTGAGAATTTA
TTGTTCACCGATGAAAATGACAATTTGGAAATTAAAATAATTGATTTTGG
ATTTGCACGGCTAAAGCCACCGGATAATCAGCCCCTGAAGACTCCATGCT
TCACCCTTCATTATGCCGCCCCAGAGCTCTTGAATCAGAACGGCTACGAT
GAGTCCTGTGACCTGTGGAGCTTGGGCGTCATTTTGTACACAATGTTGTC
AGGACAGGTTCCCTTCCAATCTCATGACCGAAGTTTGACGTGTACCAGCG
CGGTGGAAATCATGAAGAAAATTAAAAAGGGAGATTTCTCCTTTGAAGGA
GAAGCCTGGAAGAATGTATCCCAAGAGGCTAAAGATTTGATCCAAGGACT
TCTCACAGTAGATCCAAACAAAAGGCTTAAAATGTCTGGCTTGAGGTACA
ATGAATGGCTACAAGATGGAAGTCAGCTGTCCTCCAATCCTCTGATGACT
CCGGATATTCTAGGATCTTCCGGAGCTGCCGTGCATACCTGTGTGAAAGC
AACCTTCCACGCCTTTAACAAATACAAGAGAGAGGGGTTTTGCCTTCAGA
ATGTTGATAAGGCCCCTTTGGCTAAGAGAAGAAAAATGAAAAAGACTAGC
ACCAGTACCGAGACGCGCAGCAGTTCCAGTGAGAGTTCCCATTCTTCTTC
CTCTCATTCTCACGGTAAAACTACACCCACCAAGACACTGCAGCCCAGCA
ATCCTGCCGACAGCAATAACCCGGAGACCCTCTTCCAGTTCTCGGACTCA
GTAGCTCATCACCATCACCATCACTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMEEEGGSSGGAAGTSA
DGGDGGEQLLTVKHELRTANLTGHAEKVGIENFELLKVLGTGAYGKVFLV
RKISGHDTGKLYAMKVLKKATIVQKAKTTEHTRTERQVLEHIRQSPFLVT
LHYAFQTETKLHLILDYINGGELFTHLSQRERFTEHEVQIYVGEIVLALE
HLHKLGIIYRDIKLENILLDSNGHVVLTDFGLSKEFVADETERAYSFCGT
IEYMAPDIVRGGDSGHDKAVDWWSLGVLMYELLTGASPFTVDGEKNSQAE
ISRRILKSEPPYPQEMSALAKDLIQRLLMKDPKKRLGCGPRDADEIKEHL
FFQKINWDDLAAKKVPAPFKPVIRDELDVSNFAEEFTEMDPTYSPAALPQ
SSEKLFQGYDFVAPSILFKRNAAVIDPLQFHMGVERPGVTNVARSAMMKD
SPFYQHYDLDLKDKPLGEGSFSICRKCVHKKSNQAFAVKIISKRMEANTQ
KEITALKLCEGHPNIVKLHEVFHDQLHTFLVMELLNGGELFERIKKKKHF
SETEASYIMRKLVSAVSHMHDVGVVHRDLKPENLLFTDENDNLEIKIIDF
GFARLKPPDNQPLKTPCFTLHYAAPELLNQNGYDESCDLWSLGVILYTML
SGQVPFQSHDRSLTCTSAVEIMKKIKKGDFSFEGEAWKNVSQEAKDLIQG
LLTVDPNKRLKMSGLRYNEWLQDGSQLSSNPLMTPDILGSSGAAVHTCVK
ATFHAFNKYKREGFCLQNVDKAPLAKRRKMKKTSTSTETRSSSSESSHSS
SSHSHGKTTPTKTLQPSNPADSNNPETLFQFSDSVAHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5378
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.