cDNA Clone MSK1

• Synonyms: RPS6KA5
• Plasmid: pGEX6P-1-MSK1 M398-T753.6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-MSK1 M398-T753-6His
• Genbank: AF074393
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATGGGAGTTGAACGTCCTGGAGTGACAAATGTTGCCAGGAGTGC
AATGATGAAGGACTCTCCATTCTATCAACACTATGACCTAGATTTGAAGG
ACAAACCCCTGGGAGAAGGTAGTTTTTCAATTTGTCGAAAGTGTGTGCAT
AAAAAAAGTAACCAAGCTTTTGCAGTCAAAATAATCAGCAAAAGGATGGA
AGCCAATACTCAAAAGGAAATAACAGCTCTGAAACTCTGTGAAGGACACC
CCAATATTGTGAAGTTGCATGAAGTTTTTCATGATCAGCTTCACACGTTT
CTAGTGATGGAACTTCTGAATGGAGGAGAACTGTTTGAGCGCATTAAGAA
AAAGAAGCACTTCAGTGAGACGGAAGCCAGCTACATCATGAGGAAGCTTG
TTTCAGCTGTAAGCCACATGCATGATGTTGGAGTGGTGCACAGGGATCTG
AAACCTGAGAATTTATTGTTCACCGATGAAAATGACAATTTGGAAATTAA
AATAATTGATTTTGGATTTGCACGGCTAAAGCCACCGGATAATCAGCCCC
TGAAGACTCCATGCTTCACCCTTCATTATGCCGCCCCAGAGCTCTTGAAT
CAGAACGGCTACGATGAGTCCTGTGACCTGTGGAGCTTGGGCGTCATTTT
GTACACAATGTTGTCAGGACAGGTTCCCTTCCAATCTCATGACCGAAGTT
TGACGTGTACCAGCGCGGTGGAAATCATGAAGAAAATTAAAAAGGGAGAT
TTCTCCTTTGAAGGAGAAGCCTGGAAGAATGTATCCCAAGAGGCTAAAGA
TTTGATCCAAGGACTTCTCACAGTAGATCCAAACAAAAGGCTTAAAATGT
CTGGCTTGAGGTACAATGAATGGCTACAAGATGGAAGTCAGCTGTCCTCC
AATCCTCTGATGACTCCGGATATTCTAGGATCTTCCGGAGCTGCCGTGCA
TACCTGTGTGAAAGCAACCTTCCACGCCTTTAACAAATACAAGAGAGAGG
GGTTTTGCCTTCAGAATGTTGATAAGGCCCCTTTGGCTAAGAGAAGAAAA
ATGAAAAAGACTAGCACCAGTACCCACCATCACCATCACCATTAGgcggc
cgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMGVERPGVTNVARSAM
MKDSPFYQHYDLDLKDKPLGEGSFSICRKCVHKKSNQAFAVKIISKRMEA
NTQKEITALKLCEGHPNIVKLHEVFHDQLHTFLVMELLNGGELFERIKKK
KHFSETEASYIMRKLVSAVSHMHDVGVVHRDLKPENLLFTDENDNLEIKI
IDFGFARLKPPDNQPLKTPCFTLHYAAPELLNQNGYDESCDLWSLGVILY
TMLSGQVPFQSHDRSLTCTSAVEIMKKIKKGDFSFEGEAWKNVSQEAKDL
IQGLLTVDPNKRLKMSGLRYNEWLQDGSQLSSNPLMTPDILGSSGAAVHT
CVKATFHAFNKYKREGFCLQNVDKAPLAKRRKMKKTSTSTHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5376
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 68297 Daltons