cDNA Clone RIPK2

• Plasmid: pFastBac.HTb RICK/RIPK2 2-199
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-RIPK2 2-199
• Genbank: NM_003821
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccAACGGGGAGGCCATCTGCAGCGCCCTGCCCACCATTCCCTACCACAAACTCGCCGACCTGCGCTACCTGAGCCGCGGCGCCTCTGGCACTGTGTCGTCCGCCCGCCACGCAGACTGGCGCGTCCAGGTGGCCGTGAAGCACCTGCACATCCACACTCCGCTGCTCGACAGTGAAAGAAAGGATGTCTTAAGAGAAGCTGAAATTTTACACAAAGCTAGATTTAGTTACATTCTTCCAATTTTGGGAATTTGCAATGAGCCTGAATTTTTGGGAATAGTTACTGAATACATGCCAAATGGA
TCATTAAATGAACTCCTACATAGGAAAACTGAATATCCTGATGTTGCTTG
GCCATTGAGATTTCGCATCCTGCATGAAATTGCCCTTGGTGTAAATTACC
TGCACAATATGACTCCTCCTTTACTTCATCATGACTTGAAGACTCAGAAT
ATCTTATTGGACAATGAATTTCATGTTAAGATTGCAGATTTTGGTTTATC
AAAGTGGCGCATGATGTCCCTCTCACAGTCACGAAGTAGCAAATCTGCAC
CAGAAGGAGGGACAATTATCTATATGCCACCTGAAAACTATGAATAAgcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSNGEAICSALPTIPYHKLADLRY
LSRGASGTVSSARHADWRVQVAVKHLHIHTPLLDSERKDVLREAEILHKA
RFSYILPILGICNEPEFLGIVTEYMPNGSLNELLHRKTEYPDVAWPLRFR
ILHEIALGVNYLHNMTPPLLHHDLKTQNILLDNEFHVKIADFGLSKWRMM
SLSQSRSSKSAPEGGTIIYMPPENYE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5317
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 25939 Daltons