cDNA Clone Cortactin A

• Plasmid: pEBG6P-1 Cortactin A
• Parent Plasmid: pEBG6P
• Expressed: GST-Cortactin A
• Genbank: NM_005231
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGTGGAAAGCTTCAGCAGGCCACGCTGTGTCCATCGCCCAGGATGACGCGGGGGCCGATGACTGGGAGACCGACCCTGATTTTGTGAATGATGTGAGTGAGAAGGAGCAAAGATGGGGTGCCAAGACGGTGCAGGGCTCCGGGCACCAGGAGCATATCAACATACACAAGCTGAGGGAGAATGTCTTTCAAGAGCATCAGACCCTTAAGGAGAAGGAACTTGAAACAGGACCAAAAGCTTCCCATGGCTATGGAGGGAAATTTGGTGTGGAACAAGACCGAATGGATAAGTCAGCTGTC
GGCCACGAATATCAGTCGAAACTTTCCAAGCACTGCTCGCAGGTGGACTC
GGTCCGTGGCTTCGGAGGCAAGTTTGGTGTCCAGATGGACAGAGTTGATC
AGTCTGCTGTAGGCTTTGAATACCAGGGGAAGACTGAGAAGCATGCCTCC
CAGAAAGACTACTCCAGTGGTTTTGGCGGCAAGTATGGCGTGCAGGCCGA
CCGAGTAGACAAGAGCGCGGTGGGCTTCGACTACCAGGGCAAGACGGAGA
AGCACGAGTCACAGAGAGATTACTCCAAAGGTTTCGGCGGCAAATACGGT
ATCGACAAGGACAAAGTGGATAAGAGCGCCGTTGGCTTTGAGTATCAAGG
CAAAACGGAGAAGCACGAGTCCCAGAAAGACTATGTGAAAGGGTTTGGAG
GAAAATTTGGTGTGCAGACAGACAGACAAGACAAATGTGCCCTTGGCTGG
GATCACCAGGAGAAATTGCAGCTGCATGAATCCCAAAAAGATTATAAGAC
TGGTTTTGGAGGCAAATTCGGTGTTCAGTCGGAGAGGCAGGACTCCGCTG
CTGTGGGGTTTGATTACAAGGAGAAGCTGGCCAAGCACGAGTCCCAGCAA
GACTACTCCAAAGGATTCGGCGGGAAGTATGGGGTGCAGAAGGATCGGAT
GGATAAGAATGCGTCAACCTTTGAGGATGTCACCCAGGTGTCCTCTGCCT
ACCAGAAGACAGTACCTGTCGAAGCTGTGACCAGCAAAACAAGTAACATC
AGAGCTAACTTTGAAAACCTCGCTAAGGAGAAAGAGCAGGAGGACAGGCG
GAAGGCGGAGGCGGAGAGAGCCCAGCGGATGGCCAAGGAGCGGCAGGAGC
AGGAAGAGGCCAGGAGGAAGCTGGAGGAGCAAGCCAGAGCCAAAACGCAA
ACGCCCCCTGTGTCGCCCGCACCTCAGCCAACCGAGGAGAGGCTGCCCTC
GAGCCCCGTCTATGAGGATGCGGCTTCCTTCAAGGCAGAGCTGAGCTACA
GAGGCCCTGTGAGTGGGACGGAGCCGGAGCCCGTGTACAGCATGGAGGCC
GCTGACTACCGAGAGGCCAGCAGCCAGCAGGGCCTGGCCTATGCCACAGA
GGCTGTCTATGAAAGCGCAGAGGCCCCGGGCCACTATCCCGCAGAGGACA
GCACCTACGATGAGTACGAGAACGATCTGGGGATCACAGCCGTCGCCCTG
TACGACTACCAGGCTGCGGGCGATGATGAGATCTCATTTGACCCTGATGA
CATCATCACCAACATCGAGATGATTGACGACGGCTGGTGGCGCGGGGTGT
GCAAGGGCCGGTACGGGCTCTTCCCAGCCAACTATGTGGAGCTGCGGCAG
TAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMWKASAGHAVSIA
QDDAGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVF
QEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAVGHEYQSKLSKHCS
QVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYG
VQADRVDKSAVGFDYQGKTEKHESQRDYSKGFGGKYGIDKDKVDKSAVGF
EYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQK
DYKTGFGGKFGVQSERQDSAAVGFDYKEKLAKHESQQDYSKGFGGKYGVQ
KDRMDKNASTFEDVTQVSSAYQKTVPVEAVTSKTSNIRANFENLAKEKEQ
EDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKTQTPPVSPAPQPTEE
RLPSSPVYEDAASFKAELSYRGPVSGTEPEPVYSMEAADYREASSQQGLA
YATEAVYESAEAPGHYPAEDSTYDEYENDLGITAVALYDYQAAGDDEISF
DPDDIITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5256
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express Cortactin A with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 88964 Daltons