cDNA Clone GAK

• Plasmid: pFBDual Cyclin G1/6His.GAK 26-392
• Parent Plasmid: pFastbacDual
• Expressed: Cyclin G1/6His-GAK 26-392
• Genbank: NM_004060 / NM_005255.3
• Tag: 6His
• Species: Human

Sequence of Insert

DNA sequence of insert 1 (Cyclin G1) :
ctcgagGAAACCATGATAGAGGTACTGACAACAACTGACTCTCAGAAACT
GCTACACCAGCTGAATGCCCTGTTGGAACAGGAGTCTAGATGTCAGCCAA
AGGTCTGTGGTTTGAGACTAATTGAGTCTGCACACGATAATGGCCTCAGA
ATGACTGCAAGACTAAGGGACTTTGAAGTAAAAGATCTTCTTAGTCTAAC
TCAGTTCTTTGGCTTTGACACAGAGACATTTTCTCTAGCTGTGAATTTAC
TGGACAGATTCCTGTCTAAAATGAAGGTACAGCCCAAGCACCTTGGGTGT
GTTGGACTGAGCTGCTTTTATTTGGCTGTAAAATCAATAGAAGAGGAAAG
GAATGTCCCATTGGCAACTGACTTGATCCGAATAAGTCAATATAGGTTTA
CGGTTTCAGACTTGATGAGAATGGAAAAGATTGTATTGGAGAAGGTGTGT
TGGAAAGTCAAAGCTACTACTGCCTTTCAATTTCTGCAACTGTATTATTC
ACTCCTTCAAGAGAACTTGCCACTTGAAAGGAGAAATAGCATTAATTTTG
AAAGACTAGAAGCTCAACTGAAGGCATGTCATTGCAGGATCATATTTTCT
AAAGCAAAGCCTTCTGTGTTGGCATTGTCTATCATTGCATTAGAGATCCA
AGCACAGAAGTGTGTAGAGTTAACAGAAGGAATAGAATGTCTTCAGAAAC
ATTCCAAGATAAATGGCAGAGATCTGACCTTCTGGCAAGAGCTTGTATCC
AAATGTTTAACTGAATATTCATCAAATAAGTGTTCCAAACCAAATGTTCA
GAAGTTGAAATGGATTGTTTCTGGGCGTACTGCACGGCAATTGAAGCATA
GCTACTACAGAATAACTCACCTTCCAACAATTCCTGAAATGGTCCCTTAA
ggtacc

DNA sequence of insert 2 (6His.GAK 26-392):gaattcGAAACCATGCACCATCACCATCACCATAGTGACTTCGTGGGGCA
GACGGTGGAACTGGGCGAGCTGCGGCTGCGGGTGCGGCGGGTCCTGGCCG
AAGGAGGGTTTGCATTTGTGTATGAAGCTCAAGATGTGGGGAGTGGCAGA
GAGTATGCATTAAAGAGGCTATTATCCAATGAAGAGGAAAAGAACAGAGC
CATCATTCAAGAAGTTTGCTTCATGAAAAAGCTTTCCGGCCACCCGAACA
TTGTCCAGTTTTGTTCTGCAGCGTCTATAGGAAAAGAGGAGTCAGACACG
GGGCAGGCTGAGTTCCTCTTGCTCACAGAGCTCTGTAAAGGGCAGCTGGT
GGAATTTTTGAAGAAAATGGAATCTCGAGGCCCCCTTTCGTGCGACACGG
TTCTGAAGATCTTCTACCAGACGTGCCGCGCCGTGCAGCACATGCACCGG
CAGAAGCCGCCCATCATCCACAGGGACCTCAAGGTTGAGAACTTGTTGCT
TAGTAACCAAGGGACCATTAAGCTGTGTGACTTTGGCAGTGCCACGACCA
TCTCGCACTACCCTGACTACAGCTGGAGCGCCCAGAGGCGAGCCCTGGTG
GAGGAAGAGATCACGAGGAATACAACACCAATGTATAGAACACCAGAAAT
CATAGACTTGTATTCCAACTTCCCGATCGGCGAGAAGCAGGATATCTGGG
CCCTGGGCTGCATCTTGTACCTGCTGTGCTTCCGGCAGCACCCTTTTGAG
GATGGAGCGAAACTTCGAATAGTCAATGGGAAGTACTCGATCCCCCCGCA
CGACACGCAGTACACGGTCTTCCACAGCCTCATCCGCGCCATGCTGCAGG
TGAACCCGGAGGAGCGGCTGTCCATCGCCGAGGTGGTGCACCAGCTGCAG
GAGATCGCGGCCGCCCGCAACGTGAACCCCAAGTCTCCCATCACAGAGCT
CCTGGAGCAGAATGGAGGCTACGGGAGCGCCACACTGTCCCGAGGGCCAC
CCCCTCCCGTGGGCCCCGCTGGCAGTGGCTACAGTGGAGGCCTGGCGCTG
GCGGAGTACGACCAGCCGTATGGCGGCTTCCTGGACATTCTGCGGGGTGG
GACAGAGCGGCTCTTCACCAACCTCAAGGACACCTGAactagt

Amino Acid Sequence

Amino acid sequence of expressed protein 1 (Cyclin G1-p10 promoter) :
MIEVLTTTDSQKLLHQLNALLEQESRCQPKVCGLRLIESAHDNGLRMTAR
LRDFEVKDLLSLTQFFGFDTETFSLAVNLLDRFLSKMKVQPKHLGCVGLS
CFYLAVKSIEEERNVPLATDLIRISQYRFTVSDLMRMEKIVLEKVCWKVK
ATTAFQFLQLYYSLLQENLPLERRNSINFERLEAQLKACHCRIIFSKAKP
SVLALSIIALEIQAQKCVELTEGIECLQKHSKINGRDLTFWQELVSKCLT
EYSSNKCSKPNVQKLKWIVSGRTARQLKHSYYRITHLPTIPEMVP*

Amino acid sequence of expressed protein 2 (6His.GAK 26-392-Polyhedrin promoter) : MHHHHHHSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDVGSGREYAL
KRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAE
FLLLTELCKGQLVEFLKKMESRGPLSCDTVLKIFYQTCRAVQHMHRQKPP
IIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEI
TRNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAK
LRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQEIAA
ARNVNPKSPITELLEQNGGYGSATLSRGPPPPVGPAGSGYSGGLALAEYD
QPYGGFLDILRGGTERLFTNLKDT*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5223
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus dual expression plasmid to express untagged Cyclin G1 and 6His-tagged GAK 26-392.
• Low Temp: No