cDNA Clone Cortactin B

• Plasmid: pEBG6P-1 Cortactin B
• Parent Plasmid: pEBG6P
• Expressed: GST-Cortactin B
• Genbank: NM_138565
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGTGGAAAGCTTCAGCAGGCCACGCTGTGTCCATCGCCCAGGATGACGCGGGGGCCGATGACTGGGAGACCGACCCTGATTTTGTGAATGATGTGAGTGAGAAGGAGCAAAGATGGGGTGCCAAGACGGTGCAGGGCTCCGGGCACCAGGAGCATATCAACATACACAAGCTGAGGGAGAATGTCTTTCAAGAGCATCAGACCCTTAAGGAGAAGGAACTTGAAACAGGACCAAAAGCTTCCCATGGCTATGGAGGGAAATTTGGTGTGGAACAAGACCGAATGGATAAGTCAGCTGTC
GGCCACGAATATCAGTCGAAACTTTCCAAGCACTGCTCGCAGGTGGACTC
GGTCCGTGGCTTCGGAGGCAAGTTTGGTGTCCAGATGGACAGAGTTGATC
AGTCTGCTGTAGGCTTTGAATACCAGGGGAAGACTGAGAAGCATGCCTCC
CAGAAAGACTACTCCAGTGGTTTTGGCGGCAAGTATGGCGTGCAGGCCGA
CCGAGTAGACAAGAGCGCGGTGGGCTTCGACTACCAGGGCAAGACGGAGA
AGCACGAGTCACAGAGAGATTACTCCAAAGGTTTCGGCGGCAAATACGGT
ATCGACAAGGACAAAGTGGATAAGAGCGCCGTTGGCTTTGAGTATCAAGG
CAAAACGGAGAAGCACGAGTCCCAGAAAGACTATGTGAAAGGGTTTGGAG
GAAAATTTGGTGTGCAGACAGACAGACAAGACAAATGTGCCCTTGGCTGG
GATCACCAGGAGAAATTGCAGCTGCATGAATCCCAAAAAGACTACTCCAA
AGGATTCGGCGGGAAGTATGGGGTGCAGAAGGATCGGATGGATAAGAATG
CGTCAACCTTTGAGGATGTCACCCAGGTGTCCTCTGCCTACCAGAAGACA
GTACCTGTCGAAGCTGTGACCAGCAAAACAAGTAACATCAGAGCTAACTT
TGAAAACCTCGCTAAGGAGAAAGAGCAGGAGGACAGGCGGAAGGCGGAGG
CGGAGAGAGCCCAGCGGATGGCCAAGGAGCGGCAGGAGCAGGAAGAGGCC
AGGAGGAAGCTGGAGGAGCAAGCCAGAGCCAAAACGCAAACGCCCCCTGT
GTCGCCCGCACCTCAGCCAACCGAGGAGAGGCTGCCCTCGAGCCCCGTCT
ATGAGGATGCGGCTTCCTTCAAGGCAGAGCTGAGCTACAGAGGCCCTGTG
AGTGGGACGGAGCCGGAGCCCGTGTACAGCATGGAGGCCGCTGACTACCG
AGAGGCCAGCAGCCAGCAGGGCCTGGCCTATGCCACAGAGGCTGTCTATG
AAAGCGCAGAGGCCCCGGGCCACTATCCCGCAGAGGACAGCACCTATGAT
GAGTACGAGAACGATCTGGGGATCACAGCCGTCGCCCTGTACGACTACCA
GGCTGCGGGCGATGATGAGATCTCATTTGACCCTGATGACATCATCACCA
ACATCGAGATGATTGACGACGGCTGGTGGCGCGGGGTGTGCAAGGGCCGG
TACGGGCTCTTCCCAGCCAACTATGTGGAGCTGCGGCAGTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMWKASAGHAVSIA
QDDAGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVF
QEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAVGHEYQSKLSKHCS
QVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYG
VQADRVDKSAVGFDYQGKTEKHESQRDYSKGFGGKYGIDKDKVDKSAVGF
EYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQK
DYSKGFGGKYGVQKDRMDKNASTFEDVTQVSSAYQKTVPVEAVTSKTSNI
RANFENLAKEKEQEDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKTQ
TPPVSPAPQPTEERLPSSPVYEDAASFKAELSYRGPVSGTEPEPVYSMEA
ADYREASSQQGLAYATEAVYESAEAPGHYPAEDSTYDEYENDLGITAVAL
YDYQAAGDDEISFDPDDIITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5190
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express with N-terminal GST (contains PreScission Protease site); N.B. Sequence difference compared with NM_138565 CDS: ; c1347t (silent in Y449); This change present in two ESTs from two different libraries.; ; Estimated MW of expressed protein : 84847 Daltons