cDNA Clone SLX4 (yeast)

• Plasmid: pEBG6P-1 SLX4 448-748end
• Parent Plasmid: pEBG6P
• Genbank: Z73307
• Species: Yeast

Sequence of Insert

ggatccCCATTAGCCACCGACACGATAGGCAGCACACAGTTTTTCACTCCAAACACATCGCCTTTGGATGGGATAATCGATTTAACACAGGAATCATTCAAAGCTGTTCGATCGTTAATATCACCTTTGAAAGTAGAAAACAACAAAACAGGGGTAACATCACAAGCATCAAATCAGGTTCAGGTACCAGCTACAAGAACCCCTACAATTATTCCTCAAAAGAACCTTACTACAACTTTAAAAACTGAAGAAGAAAAGAATAACATAGGAAGTTCCATCAGAGTTAAATTATTGCAAGAAAGCGTA
GTTAAGCTAAACCCCAAACTGGTGAAACATAACTTTTATAGAGTTGAGGC
AAATGATTCTGAAGAAGAGGAGACAGAATTTGATGACCAATTCTGCATTG
CTGATATCCAATTGGTAGATTCCTCCAAAATTTCGACTAAAGATTCTACA
CAAAATCCTACTACTAGCAACGACATTATTGATACCTCTGCAGCTAGTTC
CATAGCTTCTCCCGAGAAATTTTGTGAGATCATGATGTCCCAATCAATGA
AAGAGCTTCGACAAAGCTTGAAAACAGTGGGGTTGAAACCTATGAGGACG
AAGGTTGAAATCATTCAATCCTTACAAACTGCATCTCAAATTCTGTCCAC
AGCTAATCCAGATAATAAAGGTGAGCATGGTGGGGTGGCAAATTTCAGTA
AGATAGAAATATTTGATCATTTAACCGAACTAATTGAAGCCTTCCCTGAT
TTTTTGGAGCGAATATATACCTTTGAACCCATTCCGCTAAATGAGCTAAT
TGAAAAGCTGTTCAGCGCCGAGCCATTTGTTTCGCAAATCGACGAAATGA
CCATCAGAGAATGGGCCGATGTTCAAGGAATATGTCTGAGAAACGATAAA
AAGTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSPLATDTIGSTQFF
TPNTSPLDGIIDLTQESFKAVRSLISPLKVENNKTGVTSQASNQVQVPAT
RTPTIIPQKNLTTTLKTEEEKNNIGSSIRVKLLQESVVKLNPKLVKHNFY
RVEANDSEEEETEFDDQFCIADIQLVDSSKISTKDSTQNPTTSNDIIDTS
AASSIASPEKFCEIMMSQSMKELRQSLKTVGLKPMRTKVEIIQSLQTASQ
ILSTANPDNKGEHGGVANFSKIEIFDHLTELIEAFPDFLERIYTFEPIPL
NELIEKLFSAEPFVSQIDEMTIREWADVQGICLRNDKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5116
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 60996 Daltons; ; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.