cDNA Clone SLX4 (yeast)

• Plasmid: pGEX6P-3-SLX4 448-748end
• Parent Plasmid: pGEX6P3
• Genbank: Z73307
• Species: Yeast

Sequence of Insert

ggatccCCATTAGCCACCGACACGATAGGCAGCACACAGTTTTTCACTCCAAACACATCGCCTTTGGATGGGATAATCGATTTAACACAGGAATCATTCAAAGCTGTTCGATCGTTAATATCACCTTTGAAAGTAGAAAACAACAAAACAGGGGTAACATCACAAGCATCAAATCAGGTTCAGGTACCAGCTACAAGAACCCCTACAATTATTCCTCAAAAGAACCTTACTACAACTTTAAAAACTGAAGAAGAAAAGAATAACATAGGAAGTTCCATCAGAGTTAAATTATTGCAAGAAAGCGTA
GTTAAGCTAAACCCCAAACTGGTGAAACATAACTTTTATAGAGTTGAGGC
AAATGATTCTGAAGAAGAGGAGACAGAATTTGATGACCAATTCTGCATTG
CTGATATCCAATTGGTAGATTCCTCCAAAATTTCGACTAAAGATTCTACA
CAAAATCCTACTACTAGCAACGACATTATTGATACCTCTGCAGCTAGTTC
CATAGCTTCTCCCGAGAAATTTTGTGAGATCATGATGTCCCAATCAATGA
AAGAGCTTCGACAAAGCTTGAAAACAGTGGGGTTGAAACCTATGAGGACG
AAGGTTGAAATCATTCAATCCTTACAAACTGCATCTCAAATTCTGTCCAC
AGCTAATCCAGATAATAAAGGTGAGCATGGTGGGGTGGCAAATTTCAGTA
AGATAGAAATATTTGATCATTTAACCGAACTAATTGAAGCCTTCCCTGAT
TTTTTGGAGCGAATATATACCTTTGAACCCATTCCGCTAAATGAGCTAAT
TGAAAAGCTGTTCAGCGCCGAGCCATTTGTTTCGCAAATCGACGAAATGA
CCATCAGAGAATGGGCCGATGTTCAAGGAATATGTCTGAGAAACGATAAA
AAGTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPLATDTIGSTQFFTPNTSP
LDGIIDLTQESFKAVRSLISPLKVENNKTGVTSQASNQVQVPATRTPTII
PQKNLTTTLKTEEEKNNIGSSIRVKLLQESVVKLNPKLVKHNFYRVEAND
SEEEETEFDDQFCIADIQLVDSSKISTKDSTQNPTTSNDIIDTSAASSIA
SPEKFCEIMMSQSMKELRQSLKTVGLKPMRTKVEIIQSLQTASQILSTAN
PDNKGEHGGVANFSKIEIFDHLTELIEAFPDFLERIYTFEPIPLNELIEK
LFSAEPFVSQIDEMTIREWADVQGICLRNDKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5113
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Estimated MW of expressed protein : 60386 Daltons; ; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.