cDNA Clone SLX4 (yeast)

• Plasmid: pGEX6P-3-SLX4 1-300
• Parent Plasmid: pGEX6P3
• Genbank: Z73307
• Species: Yeast

Sequence of Insert

ggatccATGGAACTTCAGAGGGCACAACGGAATTTGAAGTTTTTACAAAACGAGGATTATGTGAACGTTACCGATCAAACTAACCTGAATGGAGAGAGCCAGAATGCTTACTCTTTAGGAATGGAAACTCAAGTACCGGAAATGCAGTTTTCCTTATCATCTGATGATGATTCAATAGGTACGCAAGTAAAAAGCGTAACTGCACAAAAATCGCCTATGACGCAAGAGACTACGAAAAACGATACAGAAAGAAATAAAGATGTTGATAAAAGTTGTAACCCAGTCAGCACTTCCCATCCCGATTTA
GGCGGATCAAATATCGAGGAGAACATATTCATTAACACTCAAATTCAAAG
CCGACTTGATGACGCAGAGGAAGAAACAAATCTTAAGTTAAAACTTGAAA
AATTCAAGTATTCGTTCAAGAGTAGTAACGCAGATGATACCCACAGCAAT
GCAAATGTAACAGCTAAAAGAAGACCAGCAATAAGGAAGGCTAATTCGAA
ACTCAAAACAAAACCTAAAACTAAACGAGACCCTAAAATTATCAAAAATA
TAACTGATTTCAATATTAATAACTACGAACGTTCAAGGACTGCCAGCTTA
TTAAAGCAATTATCAGGTAAGCACAAGAAGGTTTTGGACATAATAAAGAC
GCAAAATGAAGGAAACAGTGATAAACCTCCCAGAGCAAGGAATAACAAGG
GTGAAAAAGCTACTTTTGATACATATAGTGAACAGGAGTGGAAAGATATT
ATGAAACTTCTTCTACAAAAGTTTCCGCAAAGTGAGGAAACGGATTTGAA
TGAAGTCCAGAAATTTTTATATGGAAGCGAAAAAAGCTCGAATTCTCTAG
ACAATCAAGAATCCTCACAACAAAGACTTTGGACAGCATCACAATTACCATAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMELQRAQRNLKFLQNEDYV
NVTDQTNLNGESQNAYSLGMETQVPEMQFSLSSDDDSIGTQVKSVTAQKS
PMTQETTKNDTERNKDVDKSCNPVSTSHPDLGGSNIEENIFINTQIQSRL
DDAEEETNLKLKLEKFKYSFKSSNADDTHSNANVTAKRRPAIRKANSKLK
TKPKTKRDPKIIKNITDFNINNYERSRTASLLKQLSGKHKKVLDIIKTQN
EGNSDKPPRARNNKGEKATFDTYSEQEWKDIMKLLLQKFPQSEETDLNEV
QKFLYGSEKSSNSLDNQESSQQRLWTASQLP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5112
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Estimated MW of expressed protein : 61019 Daltons; ; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.