cDNA Clone MLK3

• Plasmid: pGEX6P-1-MLK3
• Parent Plasmid: pGEX6P1
• Expressed: GST-MLK3
• Genbank: NM_002419
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGAGCCCTTGAAGAGCCTCTTCCTCAAGAGCCCTCTAGGGTCATGGAATGGCAGTGGCAGCGGGGGTGGTGGGGGCGGTGGAGGAGGCCGGCCTGAGGGGTCTCCAAAGGCAGCGGGTTATGCCAACCCGGTGTGGACAGCCCTGTTCGACTACGAGCCCAGTGGGCAGGATGAGCTGGCCCTGAGGAAGGGTGACCGTGTGGAGGTGCTGTCCCGGGATGCAGCCATCTCAGGAGACGAGGGCTGGTGGGCGGGCCAGGTGGGTGGCCAGGTGGGCATCTTCCCGTCCAACTATGTG
TCTCGGGGTGGCGGCCCGCCCCCCTGCGAGGTGGCCAGCTTCCAGGAGCT
GCGGCTGGAGGAGGTGATCGGCATTGGAGGCTTTGGCAAGGTGTACAGGG
GCAGCTGGCGAGGTGAGCTGGTGGCTGTGAAGGCAGCTCGCCAGGACCCC
GATGAGGACATCAGTGTGACAGCCGAGAGCGTTCGCCAGGAGGCCCGGCT
CTTCGCCATGCTGGCACACCCCAACATCATTGCCCTCAAGGCTGTGTGCC
TGGAGGAGCCCAACCTGTGCCTGGTGATGGAGTATGCAGCCGGTGGGCCC
CTCAGCCGAGCTCTGGCCGGGCGGCGCGTGCCTCCCCATGTGCTGGTCAA
CTGGGCTGTGCAGATTGCCCGTGGGATGCACTACCTGCACTGCGAGGCCC
TGGTGCCCGTCATCCACCGTGATCTCAAGTCCAACAACATTTTGCTGCTG
CAGCCCATTGAGAGTGACGACATGGAGCACAAGACCCTGAAGATCACCGA
CTTTGGCCTGGCCCGAGAGTGGCACAAAACCACACAAATGAGTGCCGCGG
GCACCTACGCCTGGATGGCTCCTGAGGTTATCAAGGCCTCCACCTTCTCT
AAGGGCAGTGACGTCTGGAGTTTTGGGGTGCTGCTGTGGGAACTGCTGAC
CGGGGAGGTGCCATACCGTGGCATTGACTGCCTTGCTGTGGCCTATGGCG
TAGCTGTTAACAAGCTCACACTGCCCATCCCATCCACCTGCCCCGAGCCC
TTCGCACAGCTTATGGCCGACTGCTGGGCGCAGGACCCCCACCGCAGGCC
CGACTTCGCCTCCATCCTGCAGCAGTTGGAGGCGCTGGAGGCACAGGTCC
TACGGGAAATGCCGCGGGACTCCTTCCATTCCATGCAGGAAGGCTGGAAG
CGCGAGATCCAGGGTCTCTTCGACGAGCTGCGAGCCAAGGAAAAGGAACT
ACTGAGCCGCGAGGAGGAGCTGACGCGAGCGGCGCGCGAGCAGCGGTCAC
AGGCGGAGCAGCTGCGGCGGCGCGAGCACCTGCTGGCCCAGTGGGAGCTA
GAGGTGTTCGAGCGCGAGCTGACGCTGCTGCTGCAGCAGGTGGACCGCGA
GCGACCGCACGTGCGCCGCCGCCGCGGGACATTCAAGCGCAGCAAGCTCC
GGGCGCGCGACGGCGGCGAGCGTATCAGCATGCCACTCGACTTCAAGCAC
CGCATCACCGTGCAGGCCTCACCCGGCCTTGACCGGAGGAGAAACGTCTT
CGAGGTCGGGCCTGGGGATTCGCCCACCTTTCCCCGGTTCCGAGCCATCC
AGTTGGAGCCTGCAGAGCCAGGCCAGGCATGGGGCCGCCAGTCCCCCCGA
CGTCTGGAGGACTCAAGCAATGGAGAGCGGCGAGCATGCTGGGCTTGGGG
TCCCAGTTCCCCCAAGCCTGGGGAAGCCCAGAATGGGAGGAGAAGGTCCC
GCATGGACGAAGCCACATGGTACCTGGATTCAGATGACTCATCCCCCTTA
GGATCTCCTTCCACACCCCCAGCACTCAATGGTAACCCCCCGCGGCCTAG
CCTGGAGCCCGAGGAGCCCAAGAGGCCTGTCCCCGCAGAGCGCGGTAGCA
GCTCTGGGACGCCCAAGCTGATCCAGCGGGCGCTGCTGCGCGGCACCGCC
CTGCTCGCCTCGCTGGGCCTTGGCCGCGACCTGCAGCCGCCGGGAGGCCC
AGGACGCGAGCGCGGGGAGTCCCCGACAACACCCCCCACGCCAACGCCCG
CGCCCTGCCCGACCGAGCCGCCCCCTTCCCCGCTCATCTGCTTCTCGCTC
AAGACGCCCGACTCCCCGCCCACTCCTGCACCCCTGTTGCTGGACCTGGG
TATCCCTGTGGGCCAGCGGTCAGCCAAGAGCCCCCGACGTGAGGAGGAGC
CCCGCGGAGGCACTGTCTCACCCCCACCGGGGACATCACGCTCTGCTCCT
GGCACCCCAGGCACCCCACGTTCACCACCCCTGGGCCTCATCAGCCGACC
TCGGCCCTCGCCCCTTCGCAGCCGCATTGATCCCTGGAGCTTTGTGTCAG
CTGGGCCACGGCCTTCTCCCCTGCCATCACCACAGCCTGCACCCCGCCGA
GCACCCTGGACCTTGTTCCCGGACTCAGACCCCTTCTGGGACTCCCCACC
TGCCAACCCCTTCCAGGGGGGCCCCCAGGACTGCAGGGCACAGACCAAAG
ACATGGGTGCCCAGGCCCCGTGGGTGCCGGAAGCGGGGCCTTGAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEPLKSLFLKSPLGSWNGS
GSGGGGGGGGGRPEGSPKAAGYANPVWTALFDYEPSGQDELALRKGDRVE
VLSRDAAISGDEGWWAGQVGGQVGIFPSNYVSRGGGPPPCEVASFQELRL
EEVIGIGGFGKVYRGSWRGELVAVKAARQDPDEDISVTAESVRQEARLFA
MLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRRVPPHVLVNWA
VQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIESDDMEHKTLKITDFG
LAREWHKTTQMSAAGTYAWMAPEVIKASTFSKGSDVWSFGVLLWELLTGE
VPYRGIDCLAVAYGVAVNKLTLPIPSTCPEPFAQLMADCWAQDPHRRPDF
ASILQQLEALEAQVLREMPRDSFHSMQEGWKREIQGLFDELRAKEKELLS
REEELTRAAREQRSQAEQLRRREHLLAQWELEVFERELTLLLQQVDRERP
HVRRRRGTFKRSKLRARDGGERISMPLDFKHRITVQASPGLDRRRNVFEV
GPGDSPTFPRFRAIQLEPAEPGQAWGRQSPRRLEDSSNGERRACWAWGPS
SPKPGEAQNGRRRSRMDEATWYLDSDDSSPLGSPSTPPALNGNPPRPSLE
PEEPKRPVPAERGSSSGTPKLIQRALLRGTALLASLGLGRDLQPPGGPGR
ERGESPTTPPTPTPAPCPTEPPPSPLICFSLKTPDSPPTPAPLLLDLGIP
VGQRSAKSPRREEEPRGGTVSPPPGTSRSAPGTPGTPRSPPLGLISRPRP
SPLRSRIDPWSFVSAGPRPSPLPSPQPAPRRAPWTLFPDSDPFWDSPPAN
PFQGGPQDCRAQTKDMGAQAPWVPEAGP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5099
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. Changes to NM_002419 ORF sequence:; c222t (silent in D74) - present in EST clones; t312c (silent in G104) - present in EST and other mRNAs; Estimated MW of expressed protein : 119436 Daltons