cDNA Clone MST2

• Plasmid: pGEX6P-1-MST2
• Parent Plasmid: pGEX6P1
• Expressed: GST-MST2
• Genbank: U60206
• Tag: GST

Sequence of Insert

ggatccGAGCAGCCGCCGGCGCCTAAGAGTAAACTAAAAAAGCTGAGTGAAGACAGTTTGACTAAGCAGCCTGAAGAAGTTTTTGATGTATTAGAGAAGCTTGGAGAAGGGTCTTATGGAAGTGTATTTAAAGCAATACACAAGGAATCCGGT
CAAGTTGTCGCAATTAAACAAGTACCTGTTGAATCAGATCTTCAGGAAAT
AATCAAAGAAATTTCCATAATGCAGCAATGTGACAGCCCATATGTTGTAA
AGTACTATGGCAGTTATTTTAAGAATACAGACCTCTGGATTGTTATGGAG
TACTGTGGCGCTGGCTCTGTCTCAGACATAATTAGATTACGAAACAAGAC
ATTAATAGAAGATGAAATTGCAACCATTCTTAAATCTACATTGAAAGGAC
TAGAATATTTGCACTTTATGAGAAAAATACACAGAGATATAAAAGCTGGA
AATATTCTCCTCAATACAGAAGGACATGCAAAATTGGCAGATTTTGGAGT
GGCTGGTCAGTTAACAGATACAATGGCAAAACGCAATACTGTAATAGGAA
CTCCATTTTGGATGGCTCCTGAGGTGATTCAAGAAATAGGCTATAACTGT
GTGGCCGACATCTGGTCCCTTGGCATTACTTCTATAGAAATGGCTGAAGG
AAAACCTCCTTATGCTGATATACATCCAATGAGGGCTATTTTTATGATTC
CCACAAATCCACCACCAACATTCAGAAAGCCAGAACTTTGGTCCGATGAT
TTCACCGATTTTGTTAAAAAGTGTTTGGTGAAGAATCCTGAGCAGAGAGC
TACTGCAACACAACTTTTACAGCATCCTTTTATCAAGAATGCCAAACCTG
TATCAATATTAAGAGACCTGATCACAGAAGCTATGGAGATCAAAGCTAAA
AGACATGAGGAACAGCAACGAGAATTGGAAGAGGAAGAAGAAAATTCGGA
TGAAGATGAGCTGGATTCCCACACCATGGTGAAGACTAGTGTGGAGAGTG
TGGGCACCATGCGGGCCACAAGCACGATGAGTGAAGGGGCCCAGACCATG
ATTGAACATAATAGCACGATGTTGGAATCCGACTTGGGGACCATGGTGAT
AAACAGTGAGGATGAGGAAGAAGAAGATGGAACTATGAAAAGAAATGCAA
CCTCACCACAAGTACAAAGACCATCTTTCATGGACTACTTTGATAAGCAA
GACTTCAAGAATAAGAGTCACGAAAACTGTAATCAGAACATGCATGAACC
CTTCCCTATGTCCAAAAACGTTTTTCCTGATAACTGGAAAGTTCCTCAAG
ATGGAGACTTTGACTTTTTGAAAAATCTAAGTTTAGAAGAACTACAGATG
CGGTTAAAAGCACTGGACCCCATGATGGAACGGGAGATAGAAGAACTTCG
TCAGAGATACACTGCGAAAAGACAGCCCATTCTGGATGCGATGGATGCAA
AGAAAAGAAGGCAGCAAAACTTTTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1421
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express MST2 with N-terminal GST