cDNA Clone SNRK

• Plasmid: pFastBac.HTa SNRK
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-SNRK
• Genbank: NM_017719
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcGCAGGATTTAAGCGAGGGTATGATGGAAAGATTGCTGGATTATATGATCTGGATAAAACCTTGGGTCGAGGCCATTTTGCCGTGGTTAAACTTGCCAGGCATGTCTTTACGGGTGAAAAGGTGGCAGTAAAAGTTATTGACAAGACAAAACTGGACACTCTAGCTACTGGTCATCTTTTCCAGGAAGTGAGATGCATGAAACTAGTGCAGCATCCTAACATCGTCCGCCTTTATGAAGTTATTGACACCCAGACCAAACTATATCTTATTCTAGAACTTGGGGATGAAGGAGATATGTTTGATTATATAATGAAACATGAGGAGGGTCTTAATGAAGACTTGCCCAAGAAGTATTTTGCTCAGATAGTTCATGCTATATCTTATTGCCATAAACTCCATGTGGTTCACAGAGACTTAAAACCAGAGAATGTAGTCTTCTTTGAAAAACAAGGTCTTGTAAAGTTGACAGACTTTGGGTTCAGCAACAAATTTCAACCAGGGAAGAAGCTCACTACAAGCTGTGGATCTCTTGCATATTCCGCTCCAGAAATTCTGCTTGGTGATGAGTATGATGCACCTGCAGTAGATATTTGGAGTCTGGGAGTGATCCTTTTCATGTTGGTGTGTGGGCAGCCGCCCTTTCAAGAAGCCAATGACAGTGAAACACTGACAATGATCATGGATTGCAAATATACAGTACCATCCCATGTGTCTAAAGAGTGTAAAGACCTAATCACACGGATGCTACAGAGAGATCCCAAGAGAAGGGCTTCTTTAGAAGAGATTGAAAATCATCCTTGGCTTCAGGGAGTGGACCCTTCACCAGCTACAAAGTATAACATTCCCCTTGTGTCATACAAAAATCTCTCGGAAGAGGAGCACAACAGCATCATTCAGCGCATGGTGCTTGGGGACATAGCGGATCGAGACGCCATTGTAGAAGCCCTGGAAACCAACAGGTATAACCATATCACAGCCACATACTTCCTGCTGGCTGAAAGGATCCTGAGAGAAAAGCAAGAGAAAGAAATACAGACCAGATCTGCAAGCCCGAGCAATATCAAGGCCCAGTTTAGGCAGTCATGGCCAACCAAAATTGATGTACCCCAGGACCTTGAGGATGACCTCACGGCCACTCCTTTGTCCCACGCGACTGTCCCTCAGTCTCCTGCTCGGGCTGCTGACAGTGTCCTCAATGGCCACAGGAGCAAAGGCCTGTGTGACTCAGCTAAGAAAGATGACCTCCCTGAGTTGGCTGGACCAGCACTCTCTACGGTGCCACCCGCAAGCTTAAAACCCACAGCCAGTGGGCGGAAGTGTCTGTTCAGGGTGGAAGAAGATGAAGAGGAAGATGAGGAGGACAAGAAACCCATGTCCCTCTCAACACAAGTGGTTTTGCGCCGGAAGCCATCTGTAACCAACCGCCTGACATCCAGGAAGAGTGCGCCCGTCCTCAACCAGATCTTTGAGGAAGGGGAATCTGATGATGAGTTTGACATGGATGAGAATCTGCCTCCCAAGTTGAGCAGGTTAAAGATGAATATAGCTTCTCCAGGTACAGTTCACAAACGCTACCACCGGAGGAAAAGTCAGGGCCGGGGCTCCAGCTGCAGTAGTTCGGAGACCAGTGATGATGATTCTGAAAGCCGGCGGCGGCTCGATAAAGATAGCGGGTTCACCTACTCCTGGCACCGACGGGATAGCAGCGAGGGGCCCCCTGGCAGTGAGGGGGATGGCGGGGGCCAGAGCAAGCCGAGCAATGCCAGTGGAGGGGTGGACAAGGCCAGCCCCAGTGAGAACAATGCTGGTGGGGGCAGTCCCTCCAGCGGCTCGGGTGGCAACCCCACCAATACATCGGGTACCACACGCCGCTGTGCCGGCCCCAGCAACTCCATGCAGCTGGCCTCTCGCAGTGCTGGGGAGCTCGTTGAGAGCCTCAAACTCATGAGCCTCTGCCTCGGCTCCCAGCTTCATGGGAGCACCAAGTACATTATTGATCCACAGAATGGCTTGTCATTTTCCAGTGTGAAAGTCCAAGAGAAATCTACGTGGAAAATGTGCATTAGCTCCACAGGGAATGCAGGGCAGGTCCCTGCAGTGGGCGGCATAAAGTTTTTCTCTGACCACATGGCAGATACCACCACTGAATTGGAACGGATAAAGAGCAAGAACCTGAAAAATAACGTGCTGCAGCTACCTCTGTGCGAAAAGACCATCTCTGTGAACATCCAGCGGAACCCTAAGGAGGGGCTGCTGTGCGCATCCAGCCCAGCCAGCTGTTGCCATGTCATCTGAgcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFAGFKRGYDGKIAGLYDLDKTLGRGHFAVVKLARHVFTGEKVAVKVIDKTKLDTLATGHLFQEVRCMKLVQHPNIVRLYEVIDTQTKLYLILELGDEGDMFDYIMKHEEGLNEDLPKKYFAQIVHAISYCHKLHVVHRDLKPENVVFFEKQGLVKLTDFGFSNKFQPGKKLTTSCGSLAYSAPEILLGDEYDAPAVDIWSLGVILFMLVCGQPPFQEANDSETLTMIMDCKYTVPSHVSKECKDLITRMLQRDPKRRASLEEIENHPWLQGVDPSPATKYNIPLVSYKNLSEEEHNSIIQRMVLGDIADRDAIVEALETNRYNHITATYFLLAERILREKQEKEIQTRSASPSNIKAQFRQSWPTKIDVPQDLEDDLTATPLSHATVPQSPARAADSVLNGHRSKGLCDSAKKDDLPELAGPALSTVPPASLKPTASGRKCLFRVEEDEEEDEEDKKPMSLSTQVVLRRKPSVTNRLTSRKSAPVLNQIFEEGESDDEFDMDENLPPKLSRLKMNIASPGTVHKRYHRRKSQGRGSSCSSSETSDDDSESRRRLDKDSGFTYSWHRRDSSEGPPGSEGDGGGQSKPSNASGGVDKASPSENNAGGGSPSSGSGGNPTNTSGTTRRCAGPSNSMQLASRSAGELVESLKLMSLCLGSQLHGSTKYIIDPQNGLSFSSVKVQEKSTWKMCISSTGNAGQVPAVGGIKFFSDHMADTTTELERIKSKNLKNNVLQLPLCEKTISVNIQRNPKEGLLCASSPASCCHVI*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1369
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N-terminal 6His tag.; ; Changes to NM_017719 CDS sequence: ; a290g (E97G)*; c346g (P116A)*; t1488c (silent in D496)*; g1758a (silent in P586); * changes also present in SNRK sequence in Sugen database.