cDNA Clone Rad6

• Plasmid: pGEX6P-Rad6
• Parent Plasmid: pGEX6P1
• Expressed: GST-Rad6
• Genbank: NM_003337
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCGACCCCGGCCCGGAGGAGGCTCATGCGGGATTTCAAGCG
GTTACAAGAGGACCCACCTGTGGGTGTCAGTGGCGCACCATCTGAAAACA
ACATCATGCAGTGGAATGCAGTTATATTTGGACCAGAAGGGACACCTTTT
GAAGATGGTACTTTTAAACTAGTAATAGAATTTTCTGAAGAATATCCAAA
TAAACCACCAACTGTTAGGTTTTTATCCAAAATGTTTCATCCAAATGTGT
ATGCTGATGGTAGCATATGTTTAGATATCCTTCAGAATCGATGGAGTCCA
ACATATGATGTATCTTCTATCTTAACATCAATTCAGTCTCTGCTGGATGA
ACCGAATCCTAACAGTCCAGCCAATAGCCAGGCAGCACAGCTTTATCAGG
AAAACAAACGAGAATATGAGAAAAGAGTTTCGGCCATTGTTGAACAAAGC
TGGAATGATTCATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20012
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for Rad6 with N-terminal GST tag (contains preScission protease site); ; Estimated MW of expressed protein : 44137 Daltons