cDNA Clone DUBA-1

• Plasmid: pMalP DUBA-1
• Parent Plasmid: pMAL6P
• Expressed: MBP-DUBA-1
• Genbank: NM_017602*
• Tag: MBP
• Species: Human

Sequence of Insert

ATGACTATACTCCCCAAAAAGAAGCCGCCGCCTCCCGACGCCGACCCCGG
CGGCACGGGCGTGGGCGGCGGCGATCGCGACCGTGACTCCGGCGTCGTGG
GGGCCCGTCCGCGAGCTTCGCCACCGCCTCAAGGCCCGCTACCAGGACCG
CCGGGCGCTCTTCATCGCTGGGCGCTGGCCGTGCCGCCTGGTGCAGTGGC
GGGTCCCCGGCCACAACAGGCTTCTCCACCTCCTTGCGGGGGCCCAGGTG
GTCCCGGCGGCGGTCCCGGCGACGCGCTGGGCGCAGCGGCGGCGGGTGTG
GGTGCCGCGGGCGTGGTGGTGGGTGTGGGTGGTGCCGTAGGCGTGGGCGG
CTGCTGCTCCGGGCCTGGGCACAGCAAGCGGCGACGTCAAGCTCCCGGGG
TTGGCGCGGTTGGCGGGGGCAGTCCCGAGCGTGAGGAGGTCGGCGCAGGC
TACAACAGTGAGGACGAGTATGAGGCGGCTGCAGCACGCATCGAGGCTAT
GGACCCTGCCACTGTCGAGCAGCAGGAGCATTGGTTTGAAAAGGCCCTAC
GAGACAAGAAGGGCTTCATCATCAAGCAGATGAAGGAGGATGGCGCCTGT
CTCTTCCGGGCTGTAGCTGACCAGGTGTATGGAGACCAGGACATGCATGA
GGTTGTGCGAAAGCATTGCATGGACTATCTGATGAAGAATGCCGACTACT
TCTCCAACTATGTCACAGAGGACTTTACCACCTACATTAACAGGAAGCGG
AAAAACAATTGCCATGGCAACCACATTGAGATGCAGGCCATGGCAGAGAT
GTACAACCGTCCTGTGGAGGTGTACCAGTACAGCACAGAACCCATCAACA
CATTCCATGGGATACATCAAAACGAGGACGAACCCATTCGTGTTAGCTAC
CATCGGAATATCCACTATAATTCAGTGGTGAATCCTAACAAGGCCACCAT
TGGTGTGGGGCTGGGCCTGCCATCATTCAAACCAGGGTTTGCAGAGCAGT
CTCTGATGAAGAATGCCATAAAAACATCGGAGGAGTCATGGATTGAACAG
CAGATGCTAGAAGACAAGAAACGGGCCACAGACTGGGAGGCCACAAATGA
AGCCATCGAGGAGCAGGTGGCTCGGGAATCCTACCTGCAGTGGTTGCGGG
ATCAGGAGAAACAGGCTCGCCAGGTCCGAGGCCCCAGCCAGCCCCGGAAA
GCCAGCGCCACATGCAGTTCGGCCACAGCAGCAGCCTCCAGTGGCCTGGA
GGAGTGGACTAGCCGGTCCCCGCGGCAGCGGAGTTCAGCCTCGTCACCTG
AGCACCCTGAGCTGCATGCTGAATTGGGCATGAAGCCCCCTTCCCCAGGC
ACTGTTTTAGCTCTTGCCAAACCTCCTTCGCCCTGTGCGCCAGGTACAAG
CAGTCAGTTCTCGGCAGGGGCCGACCGGGCAACTTCCCCCCTTGTGTCCC
TCTACCCTGCTTTGGAGTGCCGGGCCCTCATTCAGCAGATGTCCCCCTCT
GCCTTTGGTCTGAATGACTGGGATGATGATGAGATCCTAGCTTCGGTGCT
GGCAGTGTCCCAACAGGAATACCTAGACAGTATGAAGAAAAACAAAGTGC
ACAGAGACCCGCCCCCAGACAAGAGTTGA

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSMTILPKKKPPPPDADPGGTGVGGGDRDRDSGVVGARPRASPPPQGPLPGPPGALHRWALAVPPGAVAGPRPQQASPPPCGGPGGPGGGPGDALGAAAAGVGAAGVVVGVGGAVGVGGCCSGPGHSKRRRQAPGVGAVGGGSPEREEVGAGYNSEDEYEAAAARIEAMDPATVEQQEHWFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQVRGPSQPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGTSSQFSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKNKVHRDPPPDKS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20010
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid to express DUBA-1 with N-terminal MBP tag (contains preScission protease site); ; Estimated MW of expressed protein : 104749 Daltons